We narrowed to 1,091 results for: addgene
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TypeBlog Post...doi.org/10.1093/mutage/gei016I Resources on Addgene.org Addgene’s Basic Molecular Biology Protocols Over-...Antibiotic Plating Plasmids 101 eBook Resources on the Addgene blog Plasmids 101: Antibiotic Resistance Plasmids...
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How to Be an Excellent Trainee
TypeBlog Post...biology you will study. (You can also check out Addgene’s blog, eBooks, and other educational resources ...personnel! Happy training! Resources Resources on the Addgene blog Trading Spaces – Visiting Scientist Edition... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral Guide Most popular lentiviral...Resources and References More resources on the Addgene blog Viral Vectors 101: Viral Vector Elements ... This blog was written by Addgenie Brian O'Neill, with thanks to Addgenie Rebecca Hutcheson for her assistance... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...strategies for your experiment. Also learn about Addgene’s knock-in plasmids for reagents to help you knock....3390/ijms21186461 Additional Resources on the Addgene Blog Check out our CRISPR Featured Topic Page ... CRISPR Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post...lentiviral-based CRISPR libraries available at Addgene. The large packaging capacity and stable expression...s41392-024-01780-w Additional resources on the Addgene Blog Overcoming the AAV Size Limitation for CRISPR...Using the FITS Approach Additional resources at addgene.org CRISPR pooled libraries Guide to pooled libraries... -
Design Tips for Prime Editing
TypeBlog Post...programs to help you design pegRNAs. Here on the Addgene blog, we featured a guest blog by the developer...s41586-024-07259-6 Additional Resources on the Addgene blog Prime Editing: Adding Precision and Flexibility... Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources...Resources and references More resources on the Addgene blog Viral Vectors 101: Producing Your rAAV AAV... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post...Polyclonal Antibodies Resources on addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection...luck and happy binding! More resources on the Addgene blog Antibodies 101: ELISAsAntibodies 101: Selecting... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...pair. Find BiFC plasmids for your experiment at Addgene! How to Design a BiFC Experiment Much like FRET.../10.2144/000113943 Additional resources on the Addgene blog Browse our Fluorescent Protein 101 blog posts...covering an introduction to FRET Resources on Addgene.org Find fluorescent protein plasmids and resources... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...s13059-020-01973-w Additional Resources on the Addgene Blog First time CRISPR user? Check out our tips... Here Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page ... Favorite CREs Here Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic... -
DNA Purification Without a Kit
TypeBlog Post...DNA This kit-free plasmid miniprep protocol from Addgene follows a similar workflow as a column-based plasmid...in the comments! Additional resources on the Addgene blog Read our Plasmids 101 blog series Find other...our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...Nicola Patron, who shared the MoClo plant kit via Addgene. For us, transient leaf transformations are a great...PMCID: PMC2699436. Additional resources on the Addgene blog Read all of our plant biology blog posts ...Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources page... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...7th grade biology teacher. I led up to my job at Addgene, which is likely a career path none of them had...guidance and feedback. Additional resources on the Addgene blog Browse our CRISPR blog posts Download our...science communication blog posts Resources on Addgene.org Check out our comprehensive CRISPR guide Find... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch our career videos ...outdoor photographer. Additional resources on the Addgene blog Find tips for presenting a science talk Check... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...Resume Writing Resources on Addgene.org Check Out Career Opportunities at Addgene ...it's impact on human health. Resources on the Addgene Blog Learn How to Experiment with New Careers ... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing your own CRISPR... the Dish blog biobabel blog Resources on the Addgene Blog Catch Up on Your CRISPR Background with CRISPR...Methods Validate Your Genome Edit Resources on Addgene.org Find CRISPR Plasmids for Your Research Check... -
The Challenges of Cell Culture
TypeBlog Post...pharmacology (many of which are represented in Addgene's Special Collections). Arguably, oncology has benefited...Abcam Cell Culture Guidelines Resources on the Addgene Blog Learn All about Mammalian Vectors Read up...Using GFP for Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ... -
CRISPR Antimicrobials
TypeBlog Post...PMC4˚237163. Find plasmids from this paper at Addgene. 2. Bikard, David, et al. “Exploiting CRISPR-Cas...PubMed: PMID 26973885. Additional Resources on the Addgene Blog CRISPR Methods for Bacterial Genome Engineering... out of the Genome Additional Resources on Addgene.org Find CRISPR Plasmids for Your Research Read ... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...can be monomerized with an A206K mutation [2]. Addgene has many of these constructs and they are designated...PMCID: PMC3324619. Additional Resources on the Addgene Blog Read Professor Snapp's Post on mOX FPs Find...in Your Experiments Additional Resources on Addgene.org Browse our Fluorescent Protein Resources Find... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...Guide to Ebola Contribute to the Addgene Blog Resources on Addgene.org Browse Our Viral Vector Plasmids...PubMed Central PMCID: PMC1182658. Resources on the Addgene Blog Read Other Posts on Viral Vectors Check out...