... with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth. As well as being the Director...

...Career: Doer, Manager, Leader” by Charlotte Beers, divides workers into three cycles: Doers, Managers and ...

...best? What 3 things worked least well? Manager provides feedback (may be the same as self-assessment or...

...research, SciFund is really motivated more by the desire to help close the widening gap between science ...

...Gel Procedure Primer Design and PCR Design Primers. See our protocol on how to design primers . Note: (Link...sequence. FAQ How do I design primers? See our reference page on how to design primers for detailed instructions...Description of Polymerase Chain Reaction with protocol, tips and FAQ Protocols...DNA and the Taq polymerase, PCR requires free nucleotides [dNTPs; adenine (A), cytosine (C), guanine (G...the denaturing. Your 5’ and 3’ primers should be designed to have similar melting temperatures (Tm). Set...protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes. What do I do if my... to work efficiently. Taq Buffer with MgCl 2 : Provides an optimal and stable chemical environment for...

... 1: Buffer exchange using a desalting column The 10 mL Zeba Spin Desalting Columns can accommodate up ...This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using...Western Blot Protocol Introduction This protocol describes how to affinity purify recombinant antibodies ...L2884 Aprotinin, Millipore Sigma A6279 Zeba Spin Desalting Columns, 7 kDa MWCO, 10 mL, Thermo Fisher 89893... buffer exchange using a Zeba Spin 7 kDa MWCO desalting column. If the concentration of the pooled sample...into 2 columns. Twist off the 10 mL Zeba Spin Desalting Column’s bottom closure and loosen cap. Place ...Spectrophotometer to see if the sample has reached the desired concentration. If the sample concentration is still...

...your institution’s tech transfer professionals: Describe to your TTO how unprocessed agreements slow down...

...activity in the brain was measured with implanted electrodes. The Archon1 voltage reporter allows a way to...

...In the case above, you can’t costain with your desired antibodies because the pair belong to the same ...

...opposing forces or (2) the result of competing desires or (3) the presence of obstacles that need to be...

...immunocytochemistry (ICC). Not all of the solutions described here may be as effective for other techniques,...

...someone how and why they left academia. Humans are designed to pay attention and enjoy anecdotal forms of ...

...dynamic process into a static measurement and it provides limited information about what materials or organelles...

...of 173 known synthetic and natural genes.  In designing and optimizing their algorithm, they found that...

...colleagues started with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and tested a ...

... A -C compatibility grouping is an arbitrary designation, and plasmids from the same incompatibility group...

...harder to build relationships when you are more desperate or needy so you can’t wait until you imminently...

... enhances productive collaborations as well as provides visibility for grant-driven research being done...

...this post, you’ve got the knowledge to be able to design a construct for the successful and specific expression...

...H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both...