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  1. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ... a couple of tips: First, she recommends using standard BsaI in place of the high fidelity version (despite...investigating the issue and hopes to have a better understanding of the phenomenon soon.  Rest assured, as the...

  2. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ... We understand - you’re a busy scientist working hard on research, and you need the right experimental...Have a question? Our friendly and knowledgeable staff is here to help. Contact us at [email protected] or...

  3. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to bacteria, ...culture without antibiotics to give the antibiotic resistance gene time to be expressed.  However, you can ...

  4. Editor's Choice, September 2016

    Type
    Blog Post
    ...contributed by guest blogger Sean Stacey from Eastern Tennessee State University and provides a brief rundown...

  6. Inside Addgene's 10 Year Anniversary Gala

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    Blog Post
    ...ending the celebration. The party later moved to State Park, where I had a great (maybe too great!) time...last 10 years @Addgene Check out our impressive stats from 10 Years of Plasmid Sharing Or read more about...

  7. Celebrating Accomplishments in the Lab

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    Blog Post
    ... at our next group meeting and see if we should start celebrating papers and grants too with pizza or ...Choosing a Research Project Advice for Scientists Starting a Lab ...

  8. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...desired targeting homology—in frame, of course—and start tagging! Yeast-optimized fluorophores for imaging...performance in categories such as brightness, stability, and disruption of the tagged protein. Based on...

  11. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...With renewed energy and optimism that comes at the start of every new year, it is a wonderful opportunity...for-profit companies, while making use of our nonprofit status to achieve mission-driven goals of accelerating...

  12. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...back, I accepted that day and was excited for the start of my co-op in January. This excitement did not ...resources on the Addgene blog A Guide to Getting Started in Undergrad Research Tips for Writing a Good Cover...

  14. Hot Plasmids - October 2022

    Type
    Blog Post
    ...of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue voltage recording using a fast indicator...

  17. SunTag and Fluorescent Imaging

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    Blog Post
    ...SunTag is expressed at very low levels due to poor stability of the GCN4 scaffold. To increase expression, ...sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since the v4 system does ...

  19. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...hours of neuronal excitation or inhibition. This sustained activation period is beneficial for use in therapeutic...permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics poses...
Showing: 821 - 840 of 1044 results