We narrowed to 1,052 results for: sta

1. Plasmid Preps: Different Purity, Different Quantities, Different Uses

   Type

   Blog Post

   ...several hundreds of nanograms or micrograms of starting material; Gibson assembly or Golden gate assembly...success.  In conclusion, it is important for you to establish in advance how you want to utilize your plasmid...

2. Inntags: Innovative Protein Epitope Tagging

   Type

   Blog Post

   ...protein, inntags IT5 and IT6 are a great place to start. These tags are easy to use in multiple common applications...Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein...

3. Save Time and Money by Making Your Own Competent Cells

   Type

   Blog Post

   ...you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to bacteria, ...culture without antibiotics to give the antibiotic resistance gene time to be expressed.  However, you can ...

4. Microbes: Look, Listen, and Tell

   Type

   Blog Post

   ...microscope called the ‘foldscope’ (Dr. Prakash, Stanford University). Right there on the floor outside ...Microbiology and Fluorescent Protein Resources and get started!  I learned a lot about creative ways to do science...

5. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

   Type

   Blog Post

   ...Design: FRET reporter activity depends on both the distance and the orientation of the donor and acceptor ...of the nucleus depending on its phosphorylation status. The relative ratio of cytoplasmic vs. nuclear ...

6. Addgene's Top Blog Posts from 2020

   Type

   Blog Post

   ...plasmids in one cell, it might be that you can't stably maintain them in the cell. This blog post features...blogger Shravanti Suresh who studies CRISPR at Iowa State University detailed five methods for using CRISPR...

7. Designer PUF Proteins for Any RNA Target

   Type

   Blog Post

   ... a couple of tips: First, she recommends using standard BsaI in place of the high fidelity version (despite...investigating the issue and hopes to have a better understanding of the phenomenon soon.  Rest assured, as the...

8. Why Do I Need an MTA Anyway?

   Type

   Blog Post

   ... We understand - you’re a busy scientist working hard on research, and you need the right experimental...Have a question? Our friendly and knowledgeable staff is here to help. Contact us at [email protected] or...

9. Editor's Choice, September 2016

   Type

   Blog Post

   ...contributed by guest blogger Sean Stacey from Eastern Tennessee State University and provides a brief rundown...

10. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type

    Blog Post

    ...Twitter to find phages to treat an antibiotic resistant bacterial infection, she shared that tweet with...the search of phages for therapy Phage Directory started out as a directory of phage researchers and the...

11. Inside Addgene's 10 Year Anniversary Gala

    Type

    Blog Post

    ...ending the celebration. The party later moved to State Park, where I had a great (maybe too great!) time...last 10 years @Addgene Check out our impressive stats from 10 Years of Plasmid Sharing Or read more about...

12. Celebrating Accomplishments in the Lab

    Type

    Blog Post

    ... at our next group meeting and see if we should start celebrating papers and grants too with pizza or ...Choosing a Research Project Advice for Scientists Starting a Lab ...

13. Tag Your Favorite Yeast Genes with Ease

    Type

    Blog Post

    ...desired targeting homology—in frame, of course—and start tagging! Yeast-optimized fluorophores for imaging...performance in categories such as brightness, stability, and disruption of the tagged protein. Based on...

14. 2023 and 2024: Reflections and Looking Ahead

    Type

    Blog Post

    ...With renewed energy and optimism that comes at the start of every new year, it is a wonderful opportunity...for-profit companies, while making use of our nonprofit status to achieve mission-driven goals of accelerating...

15. Working 9-5: an Addgene Co-op

    Type

    Blog Post

    ...back, I accepted that day and was excited for the start of my co-op in January. This excitement did not ...resources on the Addgene blog A Guide to Getting Started in Undergrad Research Tips for Writing a Good Cover...

16. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type

    Blog Post

    ...Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience...practical guide by the workshop participants  - a testament to openness and sharing in science.  We’re excited...

17. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type

    Blog Post

    ...evaluate the impact of their work (H-index for instance), but these rely upon other researchers being ...plasmids that can be used for specific purposes. For instance, on our Optogenetics Collection page, you can ...

18. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type

    Blog Post

    ...problem is especially stark in Drosophila, where expression of Cre from the standard UAS/GAL4 system is ...

19. CASTing Off for New Shores in Human Genome Editing

    Type

    Blog Post

    ... evolution took over (with a little mutagenic assistance). Quicker CAST activity allowed certain mutated... the National Academy of Sciences of the United States of America, 114(35), E7358–E7366. https://doi.org...

20. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type

    Blog Post

    ...open doors to fresh insights and broaden your understanding, ultimately enhancing your project's potential...museums can be excellent methods to detach from the constant stream of thoughts related to lab research and...