We narrowed to 1,055 results for: sta
- 
  TypeBlog Post...can usually derive: a) the bacterial antibiotic resistance, b) the promoter that drives the insert, and ...followed by its position (number) relative to the start Methionine (Met) followed by the one letter abbreviation...
- 
  The Addgene Antibody Data HubTypeBlog Post... your research, you've probably found yourself staring at a browser full of tabs, each open to a different...anti-Lhx6.1 antibody. For a more detailed understanding of the experimental conditions in which the ...
- 
  Plasmid Preps: Different Purity, Different Quantities, Different UsesTypeBlog Post...several hundreds of nanograms or micrograms of starting material; Gibson assembly or Golden gate assembly...success. In conclusion, it is important for you to establish in advance how you want to utilize your plasmid...
- 
  Inntags: Innovative Protein Epitope TaggingTypeBlog Post...protein, inntags IT5 and IT6 are a great place to start. These tags are easy to use in multiple common applications...Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein...
- 
  Save Time and Money by Making Your Own Competent CellsTypeBlog Post...you're transforming a plasmid with ampicillin resistance. After a plasmid is introduced to bacteria, ...culture without antibiotics to give the antibiotic resistance gene time to be expressed. However, you can ...
- 
  Microbes: Look, Listen, and TellTypeBlog Post...microscope called the ‘foldscope’ (Dr. Prakash, Stanford University). Right there on the floor outside ...Microbiology and Fluorescent Protein Resources and get started! I learned a lot about creative ways to do science...
- 
  Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)TypeBlog Post...Design: FRET reporter activity depends on both the distance and the orientation of the donor and acceptor ...of the nucleus depending on its phosphorylation status. The relative ratio of cytoplasmic vs. nuclear ...
- 
  Addgene's Top Blog Posts from 2020TypeBlog Post...plasmids in one cell, it might be that you can't stably maintain them in the cell. This blog post features...blogger Shravanti Suresh who studies CRISPR at Iowa State University detailed five methods for using CRISPR...
- 
  Designer PUF Proteins for Any RNA TargetTypeBlog Post... a couple of tips: First, she recommends using standard BsaI in place of the high fidelity version (despite...investigating the issue and hopes to have a better understanding of the phenomenon soon. Rest assured, as the...
- 
  Why Do I Need an MTA Anyway?TypeBlog Post... We understand - you’re a busy scientist working hard on research, and you need the right experimental...Have a question? Our friendly and knowledgeable staff is here to help. Contact us at [email protected] or...
- 
  Editor's Choice, September 2016TypeBlog Post...contributed by guest blogger Sean Stacey from Eastern Tennessee State University and provides a brief rundown...
- 
  Phage Directory: From Phage Therapy to a Repository of Phage InformationTypeBlog Post...Twitter to find phages to treat an antibiotic resistant bacterial infection, she shared that tweet with...the search of phages for therapy Phage Directory started out as a directory of phage researchers and the...
- 
  Inside Addgene's 10 Year Anniversary GalaTypeBlog Post...ending the celebration. The party later moved to State Park, where I had a great (maybe too great!) time...last 10 years @Addgene Check out our impressive stats from 10 Years of Plasmid Sharing Or read more about...
- 
  Celebrating Accomplishments in the LabTypeBlog Post... at our next group meeting and see if we should start celebrating papers and grants too with pizza or ...Choosing a Research Project Advice for Scientists Starting a Lab ...
- 
  Tag Your Favorite Yeast Genes with EaseTypeBlog Post...desired targeting homology—in frame, of course—and start tagging! Yeast-optimized fluorophores for imaging...performance in categories such as brightness, stability, and disruption of the tagged protein. Based on...
- 
  Three Key Considerations for Precise Neuronal Targeting Using AAV TechnologiesTypeBlog Post...Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience...practical guide by the workshop participants - a testament to openness and sharing in science. We’re excited...
- 
  Working 9-5: an Addgene Co-opTypeBlog Post...back, I accepted that day and was excited for the start of my co-op in January. This excitement did not ...resources on the Addgene blog A Guide to Getting Started in Undergrad Research Tips for Writing a Good Cover...
- 
  2023 and 2024: Reflections and Looking AheadTypeBlog Post...With renewed energy and optimism that comes at the start of every new year, it is a wonderful opportunity...for-profit companies, while making use of our nonprofit status to achieve mission-driven goals of accelerating...
- 
  Increasing Your Work's Visibility with Addgene: Citation, Search, and CollectionsTypeBlog Post...evaluate the impact of their work (H-index for instance), but these rely upon other researchers being ...plasmids that can be used for specific purposes. For instance, on our Optogenetics Collection page, you can ...
- 
  Cre-ating New Methods for Site-specific Recombination in DrosophilaTypeBlog Post...problem is especially stark in Drosophila, where expression of Cre from the standard UAS/GAL4 system is ...