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We narrowed to 1,054 results for: tra

Showing: 821 - 840 of 1054 results
  1. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

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    Blog Post
    ...such as bacteria or viruses, which results in neutralization or targeted destruction.  Antibodies are composed...monoclonal antibody against a target antigen. This contrasts with polyclonal antibodies, which are mixtures...antibodies include those directed against human transcription factors such as PAX7, PAX6, AP-2 alpha and ISLET...
  2. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

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    Blog Post
    ... al. (2015). Multiplex genome engineering demonstrated in human cells They used the Golden Gate cloning...cultured human cells. While it has not yet been demonstrated, they say the vectors are also likely to work...The system now provides an efficient targeting strategy for multiplex editing of genomes. It will also...
  3. MassBioEd Career Exploration Day at Addgene

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    Blog Post
    ...learn about intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale and...much business there is in this subject.” Outreach travels with Blugene Blugene loves to visit researchers...Scientist Jessica Welch showed some highlights of his travels from 2015, including a great European photo slideshow...
  4. 500,000 Plasmids Shipped and Counting!

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    Blog Post
    ...Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture format. Our lab team...relocated to 75 Sidney Street. Then the plasmids travel to labs around the world. We've shipped plasmids... and collaborations with institutions and tech transfer offices that facilitate MTAs. The great feedback...
  5. Inntags: Innovative Protein Epitope Tagging

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    Blog Post
    ...examined the effects of GFP fusions to inntags or traditional FLAG, HA, and Myc tags. Impressively, IT6-GFP...immunoprecipitation applications, but none of the traditional tags were suitable for both applications. To ...morphology was observed with IT5 and IT6 fusions. In contrast, Flag and HA tags had especially detrimental effects...
  6. Management for Scientists: Seeking Feedback

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    Blog Post
    ...gestures are energetic.  This can take practice and training. This is important because it creates a culture...your office. Stop by instead of emailing.    Demonstrate you are open to feedback with your actions     ...listening skills such as the PAC Method. P = demonstrate patience, pause; A = ask at least one question...
  7. Science as Art

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    Blog Post
    ... as well. Beautiful microscope images which traditionally languished in the pages of thick textbooks are...Queensland, whose outstanding marine photography has attracted much attention over the past few years, including...is easy to see how these incredible images are attracting so much attention. When I spoke with Daniel about...
  8. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

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    Blog Post
    ...scientists. It takes us a decade or more for just the “training” portion of our careers. In that time we may have...organizing mentoring programs for scientists and doing training to help mentors and mentees have fulfilling, productive...wants to be for her new lab. If only every science trainee had such a dedicated person guiding the start of...
  9. Scientific Sharing in the Time of COVID-19: Databases and Resources

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    Blog Post
    ...world. To support this pace of research, new, centralized databases and centers to support the sharing ...community response initiatives. Accelerate the translational impact of research Universities have also been...creating research synergies and accelerating the translational impact of research activities. By streamlining...
  10. 2023 and 2024: Reflections and Looking Ahead

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    Blog Post
    ...released the MORF collection, a comprehensive transcription factor library, in lentiviral vectors. At 3500...conduct robust investigations into the human transcriptome. The MORF library is only one of 22 libraries...’ll also share our work in evolving Addgene’s strategic vision for the future. Over the years, we have...
  11. 6 Steps to Submitting a Resume That Gets Seen

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    Blog Post
    ... are trained to be good at learning new things—this is a strength you can draw on and demonstrate in your...often is the most effective tactic a scientist in training can adopt to ensure opportunities in the future...
  12. Cre-ating New Methods for Site-specific Recombination in Drosophila

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    Blog Post
    ...near-complete excision of the stop sequence. In contrast, Dre recombinase, the close relative of Cre, displayed... were inherited normally and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further...induced by Cre recombinase in mammalian cells. Loonstra A, Voojis M, Beverloo HB, Al Allak B, van Drunen...
  13. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

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    Blog Post
    ...Polish sub-dialect making their Skype communications extra fun) who shared first authorship on their seminal...this type of experimentation to the eventual translation to the clinic and cures. There is no doubt that...forever. He emphasized the potential of this extraordinary breakthrough. In fact, the panelists agreed ...
  14. 9 Tips to Achieve Success in Academia

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    Blog Post
    ...have: working to deadlines, writing up clear and attractive reports, being good at presentations and of course...studies. They suggest that you make time for extracurricular activities both to develop new skills and to...information about the benefits of pursuing extracurricular activities. Success evolves from passion True...
  15. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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    Blog Post
    ...(get tips for your next science talk). I do illustrations for summarizing mechanisms and applications ...part of Hans’ workload. “I am currently getting abstracts and plans together for my next 4 webinars—I have...communication with the presenters, and editing of abstracts and speaker biographies.” During the week of ...
  16. Antibodies 101: Affinity Tags

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    Blog Post
    ...blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with... glutathione resin, eliminating the need for an extra clean up step of the tag – a handy feature. If you...fusion proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications...
  17. Hot Plasmids - October 2022

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    Blog Post
    ...over Na+ and rapid photocurrent kinetics. They demonstrate the use of HcKCR1 as an optogenetic silencer ...of HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1-expressing mouse...promising avenues for future engineering to shift the spectra and improve the performance of HcKCRs. Find HcKCR...
  18. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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    Blog Post
    ...sonification. Though it helps capture weaker or more transient DNA-protein interactions, the reliance on fixation...McKay lab we study how differential access to transcriptional regulatory elements is controlled during development...permeabilize cells rather than to rely on nuclei extraction. They report that this change offers a significant...
  19. New CRISPR Web Resources and #12DaysofCRISPR Recap

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    Blog Post
    ...or A:T->G:C edits in DNA without causing double-strand breaks. Our new page shows you base editing plasmids...certainly taken notice! Our newest page in the Transcriptional Regulation category already has five blue flame...with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated gRNAs for Regulating ...
Showing: 821 - 840 of 1054 results