...levels to generate anything from individual transcription units (TUs) used to express your favorite gene-of-interest...vectors. The Plant Parts III kit has a focus on transformation and genome engineering. This is a specialized...used successfully for protein localization and transport studies in HEK cells, recombinant protein purification...Rapid Combinatorial Design for Synthetic and Traditional Biology.” ACS Synthetic Biology, vol. 5, no. ...

...Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made from rabies plasmids... the international controls prescribed by the Australia Group, for these plasmids for the majority of ...

...Michigan State University 9:36-10:29 : Kingdom's transition back to Malawi and Lilongwe University of Agriculture...-14:53 : Kingdom's current projects producing transgenic crops 14:54-23:44 : Follow up Kingdom's PhD projects... transgenic crops...

...you could directly start your digest, PCR, or transformation?  Great news. A subset of our collection has...going from streaking to a single colony to DNA extraction and quantification so that you can focus on what...280 nm and 260/230 nm ratios) and measure the concentration. Since this is a beta project, it will run for...

...such as bacteria or viruses, which results in neutralization or targeted destruction.  Antibodies are composed...monoclonal antibody against a target antigen. This contrasts with polyclonal antibodies, which are mixtures...antibodies include those directed against human transcription factors such as PAX7, PAX6, AP-2 alpha and ISLET...

... al. (2015). Multiplex genome engineering demonstrated in human cells They used the Golden Gate cloning...cultured human cells. While it has not yet been demonstrated, they say the vectors are also likely to work...The system now provides an efficient targeting strategy for multiplex editing of genomes. It will also...

...learn about intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale and...much business there is in this subject.” Outreach travels with Blugene Blugene loves to visit researchers...Scientist Jessica Welch showed some highlights of his travels from 2015, including a great European photo slideshow...

...Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture format. Our lab team...relocated to 75 Sidney Street. Then the plasmids travel to labs around the world. We've shipped plasmids... and collaborations with institutions and tech transfer offices that facilitate MTAs. The great feedback...

...examined the effects of GFP fusions to inntags or traditional FLAG, HA, and Myc tags. Impressively, IT6-GFP...immunoprecipitation applications, but none of the traditional tags were suitable for both applications. To ...morphology was observed with IT5 and IT6 fusions. In contrast, Flag and HA tags had especially detrimental effects...

...gestures are energetic.  This can take practice and training. This is important because it creates a culture...your office. Stop by instead of emailing.    Demonstrate you are open to feedback with your actions     ...listening skills such as the PAC Method. P = demonstrate patience, pause; A = ask at least one question...

...phage matches. Email sstrathdee@ucsd.edu if you can help! — Dr Steffanie Strathdee, 🗡️Superbug Slayer ...species of bacteria and sometimes, only a subset of strains of the same bacterial species are affected. As ...

... as well. Beautiful microscope images which traditionally languished in the pages of thick textbooks are...Queensland, whose outstanding marine photography has attracted much attention over the past few years, including...is easy to see how these incredible images are attracting so much attention. When I spoke with Daniel about...

...scientists. It takes us a decade or more for just the “training” portion of our careers. In that time we may have...organizing mentoring programs for scientists and doing training to help mentors and mentees have fulfilling, productive...wants to be for her new lab. If only every science trainee had such a dedicated person guiding the start of...

...released the MORF collection, a comprehensive transcription factor library, in lentiviral vectors. At 3500...conduct robust investigations into the human transcriptome. The MORF library is only one of 22 libraries...’ll also share our work in evolving Addgene’s strategic vision for the future. Over the years, we have...

...world. To support this pace of research, new, centralized databases and centers to support the sharing ...community response initiatives. Accelerate the translational impact of research Universities have also been...creating research synergies and accelerating the translational impact of research activities. By streamlining...

... are trained to be good at learning new things—this is a strength you can draw on and demonstrate in your...often is the most effective tactic a scientist in training can adopt to ensure opportunities in the future...

...near-complete excision of the stop sequence. In contrast, Dre recombinase, the close relative of Cre, displayed... were inherited normally and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further...induced by Cre recombinase in mammalian cells. Loonstra A, Voojis M, Beverloo HB, Al Allak B, van Drunen...

...Polish sub-dialect making their Skype communications extra fun) who shared first authorship on their seminal...this type of experimentation to the eventual translation to the clinic and cures. There is no doubt that...forever. He emphasized the potential of this extraordinary breakthrough. In fact, the panelists agreed ...

...have: working to deadlines, writing up clear and attractive reports, being good at presentations and of course...studies. They suggest that you make time for extracurricular activities both to develop new skills and to...information about the benefits of pursuing extracurricular activities. Success evolves from passion True...

...blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with... glutathione resin, eliminating the need for an extra clean up step of the tag – a handy feature. If you...fusion proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications...