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We narrowed to 973 results for: ARC

Showing: 421 - 430 of 973 results
  1. Hot Plasmids - February 2022

    Type
    Blog Post
    ...screening technique, researchers identified a synthetic lethal interaction between MARCH5 and WSB2 genes using...have primarily used Cas9 to knock out genes, researchers at the Genetic Pertubation Platform at the Broad...screens because of its lowered editing rate. This research aimed to increase the potential of Cas12a genetic...
  2. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post
    ...method. Read on to learn how to use it in your research! The starting point: Ligation-independent cloning...only one type of dNTP in the reaction mix, the researcher can decide exactly how far the T4 exonuclease... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...assembly, and its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless...
  3. Viral Vectors 101: Viral Applications

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    Blog Post
    ... for your experiment. Research applications Gene expression control Researchers are always wanting to ...fact, a choice delivery vector from basic science research all the way to clinical gene therapy. But how ...overexpression of all human transcription factors, barcoded libraries to trace cell lineage, and so much more...
  4. Antibodies 101: Monoclonal Antibodies

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    Blog Post
    ...-producing B cell and a myeloma cell (National Research Council (US) Committee on Methods of Producing...10.3791/54832  Lipman NS, Jackson LR, Trudel LJ, Weis-Garcia F (2005) Monoclonal Versus Polyclonal Antibodies...https://doi.org/10.1074/jbc.m116.736942  National Research Council (US) Committee on Methods of Producing...resource on the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies...
  5. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post
    ...expression of recombinant proteins for industrial or research purposes requires stable host cells (the simplest...chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis Trigger Factor), that...commercial operators, government agencies, active researchers, as well as fellow iGEM teams. We were the proud...more productive and collaborative environment for research.  We’ve also involved ourselves in extensive outreach...
  6. Antibodies 101: Secondary Antibodies

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    Blog Post
    ...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...secondary antibodies would save a good deal of it. Researchers are continuously looking for ways to improve ...providing needed flexibility in assay development. Researchers who understand how and why to use secondary antibodies...
  7. Click Biology: How to Click and Play to Explore Biology

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    Blog Post
    ...investigator at the University of Cambridge. His research focuses on protein innovations for therapeutic...Promega Plasmid Collection HaloTag Collection of research tools from Promega, including many HaloTag plasmids...Biological Building Click Biology describes the search for robust, reliable reactions, using only nature...these kinds of Click Biology reactions. For basic research, the stable and selective reactions of these tags...
  8. Antibodies 101: Polyclonal Antibodies

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    Blog Post
    ...When you’re searching for an antibody to use in your next experiment, you’ll probably notice a lot of...commonly, antibodies of the IgG isotype are used for research purposes. Isolating polyclonal antibodies from...antibodies are particularly useful for certain research applications. Many secondary antibodies are polyclonal...Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...
  9. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post
    ...things. Now, work from Connie Cepko’s lab allow researchers to activate transcription or Cre recombinase ...activation The project began with a frustration many researchers have experienced: wanting to ask and answer experimental...not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted to express a variety of ...and we’re eager to see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A...
  10. Career Coaching for Scientists: Why and Where Do I Find One?

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    Blog Post
    ...responsibility seriously. As I make my rounds speaking at research institutions and at scientific conferences, I ... is a style match for your needs can take some research.  It helps to spend some time thinking about what... Academic Labor Environment from the Future of Research Symposium Resources on the Addgene Website Check...Recent Job Openings Find Plasmids to Expedite Your Research   ...
Showing: 421 - 430 of 973 results