...of new tools came out including the single base editors, Casilio, CombiGEM, and a variety of pooled libraries...top requested plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab.... TLDR: This is a versatile mammalian expression vector that you can use to express both SpCas9 and your...resistance gene as separate peptides. PX459 This is the vector backbone. This particular backbone can be used ...Overall, this is a versatile mammalian expression vector that you can use to express both SpCas9 and your...mammalian cell lines. Alternative forms of this vector contain GFP-tagged and un-tagged versions of SpCas9...

...Structural Biology, and now serves as the Director of Student and Postdoctoral Programs at the Janelia Research...tribulations of the process, I’ve provided guidance and mentoring to several people that found my insights helpful...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no... potential collaborations, critiques of your postdoctoral research, etc. The second question is whether...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz... His interests include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization...

...simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged...522 organizations in 41 countries enriched our repository with 15,020 new plasmids — a testament to the...shared progress. But numbers tell only part of the story. This year, we made significant strides in making... our collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub reports detailing...innovation.  In response to a demand for more AAV viral vector options, we launched AAV Packaged on Request, a...

...Chapter 6: The SITN Editorial Process -Vini discusses the importance of the editorial process and peer-to-peer...and training the next generation of science communicators. In this chapter we learn about some of their...social context. Episodes are driven by narrative and story telling. Chapter 5: The SITN Blog - Vini and Amy...graduate student at Harvard University, former Co-director of Science in the News, and member of the SITN...her PhD from Harvard University, is a former Co-director of Science in the News, and is a member of the...

...molecules for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy patients...gene results in the degeneration of spinal cord motor neurons. This disease is estimated to affect anywhere...other genes. He will use fluorescent proteins to monitor the localization of these genes, CRISPR to disrupt...Peritoneal Mesothelioma  Noushin Nabavi is a Postdoctoral Fellow at the Vancouver Prostate Center and ...systems should allow Nabavi and coworkers to test inhibitors of a variety of signaling pathways for their ...

...Your Insert and Vector by Gel Purification     Now that you’ve cut your insert and vector, unfortunately...unsuccessful 1), in a vector alone plate with many more colonies than the vector + insert plate (unsuccessful..., if you were cloning a gene into an expression vector, you would want the start of the gene to end up... ligation step. 3. Ligate Your Insert into Your Vector In the ligation step, you mix your purified, cut...Resources on Addgene.org Find the Perfect Empty Vector for Your Cloning Experiments Browse Our Molecular...

...Mary O’Riordan, and Victor Di Rita, and, last, homework assignments. The instructors are leaders in the...scientists. Over the course of the class, the moderator discussed individual sections of a paper, the ...quizzes and discussion on that week’s topic by the moderators. The assignments, which consisted of reading ...course was breaking up into focus groups with a facilitator. In the focus groups, we were required to critique... of the paper. They should be able to tell your story for you and they should also be easily understood...

...same page. Ideally, these meetings include all collaborators, from the lab members who are conducting the...interesting results or ask questions to your collaborators. This helps everyone feel included in the project...materials produced from your collaboration is a key factor in maintaining the group buy-in and morale. Especially...frequently the entire group will meet. You and your collaborators should then hold each other accountable to these...and circumstances permit, think about having collaborators spend time with each other in person. One possibility...workshops are another great place to meet up with collaborators. Being able to present data from multiple aspects...

...course of the day. His talk, which focused on the history of genome engineering from ZFNs through TALENs ...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges of creating... of the book on my way to the meeting, and its history of code making and code breaking is wonderfully...addition to the poster session was a first for our repository, and I was proud to be responsible for sharing... sure what to expect in terms of interest, but visitors to the poster were impressed to see how the numbers...

... work with a team to get a lot of stuff done.  Factors contributing to job satisfaction Being a good manager...(i.e. enough pay and benefits to live on), the factors that contribute to job satisfaction are a bit surprising...studies overwhelmingly agree that these are the factors that most contribute to job satisfaction: A feeling...Availability of superiors for consultation, advice and brainstorming. Employees must understand the part they play...team miserable.   A good manager keeps these factors in mind at all times when making decisions, assignments...follow the entire series as it is published. Good introductory reference books on managing people: Skills ...