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We narrowed to 971 results for: lars;

Showing: 421 - 430 of 971 results

  1. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...colleague who preferred detailed instructions and regular check-ins, which helped them stay on track and ...resource: connecting with mentors who have made similar transitions can provide you with insights, lessons...mentoring a junior colleague. Guiding an intern, in particular, served as a great "baby project," offering a...strengths. By encouraging alignment on goals and clarifying roles, I eased the tension and improved collaboration... his PhD and a couple of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs...

  2. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...is an app that helps you find internships and scholarships in different subject areas!    2. Resume...size, legibility, and more). Tips on drafting a stellar resume and cover letter can be found on the Addgene...through. The vast majority of programs tend to ask similar questions, thus devoting some time to writing a... for them, the likelier you will end up with a stellar letter of support.        5. Application submission...number of years as you apply for internships, scholarships, fellowships, and entry-level positions post...

  3. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...themselves using computational approaches to analyze large data sets (myself included) and we are faced with... lost in the wide word of informatics. I am a molecular biologist who began coding for my thesis project...will keep you from repeating your work or making similar mistakes in the future. Stay organized Set up...for those that are just starting out, and helps clarify the role of the code. # This is a comment in python...student at University of Pennsylvania. She is particularly interested in science communication and epigenetics...

  4. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ... will provide you with an overview of general molecular biology knowledge and techniques. If you are interested...our screening overview blog post.) *Note: Two Popular Vectors for Blue White Screening Available at Addgene...of this gene, disrupting the lethality. This is similar to α-peptide DNA disruption in the blue-white screen...may contain genes that allow for the use of a particular substrate in defined medium, without which the...Resources on the Addgene Website Choose the Best Molecular Cloning Technique for You Browse Addgene's curated...

  5. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...the past several years, it seems that the more polarized (negative) the action potential of a cell, the...differentiated and quiescent it is, and the more depolarized (positive) the cell is, the more embryonic, stem...cation channel) light-activated channels to hyperpolarize tumor cells, which resulted in tumor regression...activation, it stays open for a longer duration. Hyperpolarizing chloride channel SwiChr++ - A channelrhodopsin...and gap junction protein-mediated cues control polarity during planarian regeneration." Developmental ...

  6. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...organizations with similar ideas who were also thinking about reproducibility and shared similar desires to take...talented and motivated groups and individuals with similar ideas to actually do something about it. Building...workshop. These workshops illustrate the open and modular nature of the workshop content: different parts...results, and tracking material downloads. This was particularly helpful for securing funding to support this...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    ...basics of molecular biology and cloning, since they have reasonable introductions to lots of molecular biology...quick flash back to 2004. “Shrek 2” was the most popular movie in theaters, the Razr was the hottest phone...biology,” — Reddit commentor Yay4sean, in r/molecularbiology.   Oh, to be young again! Addgenie Marcy...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing...

  8. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...academic lab, a similar situation could arise if a lab veteran who worked with a particular sample suddenly... lab management software aren’t just limited to large volume facilities; it can be useful in academic ...you sad),  someone has put in a request for a particular plasmid and now it’s time for our lab technicians...this, it is important to write loosely coupled, modular code. Use tried-and-true tools We use Python, Django...

  9. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...these mutants are not toxic to HEK cells and that similar mutations can improve the specificity of Cas9 derived...from S. aureus. This means you can likely apply similar rational mutations to your Cas9 homolog of choice...SpCas9-HF1 Kleinstiver et al and the Joung Lab similarly thought that if they could weaken sequence independent...found in eSpCas9, the effect on specificity was similar; SpCas9-HF1 generated fewer off-target cuts when...variant displays comparable on-target activity and similar or decreased off-target activity across the sites...

  10. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...as T-DDOG and Cre-DOG, respectively, repurpose popular GFP reporter lines for more sophisticated experimental...and CFP can activate certain T-DDOG constructs similarly to GFP, but commonly used red fluorescent proteins...activating gene expression. Although Cre isn’t a modular protein, Tang et. al were able to create a split...to many types of proteins. For proteins with a modular structure, constructing split variants should be... also possible to create split versions of non-modular proteins like Cre. Could Cas9-DOG be developed ...
Showing: 421 - 430 of 971 results