We narrowed to 997 results for: lars;

...IF), is an immunoassay that brings to light the cellular world. The technique allows you to ask questions...secondary antibodies on hand to mix and match with a larger collection of primary antibodies. This strategy...this step as well. Preservation and Imaging Similar to Sample Preparation, this step can involve multiple...sections of papers that have successfully used your particular antibodies and refer to the manufacturer’s recommendations...steps used in a non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody...Performing Immunofluorescence Staining. In: Methods in Molecular Biology. Springer New York, pp 299–311. https:...

...Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre for Advanced...protein to study its function is one of the most popular applications of fluorescent proteins. These fusion...Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced...Bendezú, Felipe O., et al. "Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based...291.5512 (2001): 2408-2411. PubMed PMID: 11264536. Klarenbeek, Jeffrey, et al. "Fourth-generation epac-based...dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins." Proceedings...

...lymphocyte population distinct from cellular debris and other larger cells. Note, we don’t mind the warning... scattered by that cell at a low angle (FSC) or large angle (SSC) (Figure 1). FSC values depend on the...forward at a low angle (FSC) or to the side at a large angle (SSC). FSC and SSC depend on the cell’s size...cell population will show up on an FSC/SSC plot, largely depends on cell type and laser voltage. The voltage...populations displayed. As the separation pattern largely stays the same and does not impact my ability to...

... target to specific subcellular regions, laser ablation can damage other cellular structures, and genetic...activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength...the flip of a switch. You may be familiar with popular light-sensitive ion channels used to control activities...Opto-RhoGEFs to study cell junction integrity in the vascular barrier of the endothelium, but they could be ...

...- mK+), λ– EPI300   Cloning large inserts and transformation of large plasmids. F- mcrA Δ(mrr-hsdRMS-mcrBC...genes for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency...Table 2 provides a quick reference for some of the popular strains, their genotypes, and their primary use...Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic ...coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin of Replication The ...

...Ghana and most recently got his PhD in cell and molecular biology at the University of Edinburgh where he... - 7:11 : Introduction - Ghanaian scientist's molecular biology needs and why Kwabena applied for the ...Ghana after his PhD and the need to de-mystify molecular biology. 25:51 - 30:56 : Further biology work ...current resources to develop microbiology and molecular biology research programs. 30:57 - 40:16 : Kwabena's...Microbiology Pages Find Synthetc Biology Plasmids Find Molecular Biology Resources ...

... (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein, and this fusion allows for packaging...the epigenome may make certain processes, like cellular reprogramming of iPSCs, more efficient. In general...genes of previously uncharacterized enhancers. The popular TALEN-based LITE system, which uses light to regulate...genomic targets. These two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9... use. If you want to study the effects of one particular modification for which a targeted editor, like...

...remains in the cytoplasm. Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor...Guzzo et al., 2020. Caulobacter crescentus is a popular model organism for studying the bacterial cell ...cell cycle, asymmetric cell division, and cellular differentiation, but it takes several weeks to delete ...CRISPR3 module of Streptococcus thermophilus (the popular Streptococcus pyogenes system does not work well...

...new way through the integration of science with a large set of other societal pressures including economic...p” policy is non-governmental work happening at large organizations who have to develop policies for their...policy to combat sexual harassment in academia or a large company who decides how they are going pursue regulatory...what better place to do that than at the nation’s largest integrated healthcare system? Defining your why...for skill development include leadership of extracurricular organizations, working in student government... impacts of health services research. She is particularly interested in driving scientific and technological...

...bound to the intracellular surface of the GPCR. Binding of a ligand to the extracellular surface of the...environmental conditions, cells must be able to detect extracellular stimuli. One way they do so is through G protein-coupled...number of signaling cascades, inducing different cellular responses. One of the biggest advantages of such...Bioluminescence Resonance Energy Transfer (BRET). BRET is similar to Förster Resonance Energy Transfer (FRET), in...