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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,041 results for: ins

Showing: 841 - 860 of 1041 results

  1. All Antibodies

    Type
    Collection
    ...Neuroscience : Antibodies targeting proteins for neuroscience research. Institute for Protein Innovation Collection...other types of affinity reagents such as scFvs, DARPins, Fabs, and secondary antibodies. To learn more ...Collection : Antibodies to highly conserved proteins and other difficult-to-target antigens that are intractable... represent the official views of the National Institutes of Health. Need Some Help? Browse some of our... Antibody Guide Antibody Protocols Recipient Instructions Antibody Blog Posts Addgene Help Center...

  2. Plasmid Collections

    Type
    Collection
    ... libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more....Addgene Plasmid Collections Addgene's repository contains 159,258 plasmids . We've gathered our curated ...Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral Plasmids...Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic... Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors...RESOLUTE Plasmid Collection for Solute Carrier Proteins (SLCs) Structural Genomics Consortium Zinc Finger...

  3. COVID-19 Resources

    Type
    Collection
    ...cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains a potential cleavage...credit: Maya Kostman for the Innovative Genomics Institute Addgene COVID-19 Resources The following types...distribute SARS-CoV-2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression Spike...Return to top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified as...SHERLOCK and DETECTR . Feng Zhang's lab at the Broad Institute has utilized the natural RNAse activity of the...UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad Institute has published information and protocols for utilizing...

  4. Retrovirus Plasmids

    Type
    Collection
    ...its genome and several structural and enzymatic proteins, including reverse transcriptase and integrase...genome, which can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide for...Plasmid Type Description PI 8449 pUMVC Packaging Contains MoMLV gag and pol; use with envelope plasmid Weinberg... Weinberg 12371 pCL-Eco Packaging Contains MoMLV gag, pol, and env Verma 35614 pBS-CMV-gagpol Packaging...database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo...modified version of pRX-tight Puro encoding NAT instead of PAC Iglehart 20672 MSCV-IRES-GFP MSCV For transgene...

  5. Antibody Production

    Type
    Collection
    ... distributed by Addgene were developed by the Institute for Protein Innovation (IPI). For these antibodies...more about these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage...with the plasmid(s) encoding the heavy and light chains. Antibodies are expressed for 7 days and then purified...opens in a new window) . Western Blotting (WB) Proteins are extracted from cells, separated by SDS-PAGE...thawing on ice, and then testing for loss of potency against a non-treated control in one of its recommended... Antibody Guide Antibody Protocols Recipient Instructions Antibody Blog Posts Addgene Help Center...

  7. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...and more. ID Plasmid Gene/Insert Mutations PI AAV Plasmids This table contains a general list of plasmids... handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can transduce both...AAV particles without the use of a helper virus. Instead, specific gene products can be provided by helper...All Available AAV Packaging Plasmids This table contains helper plasmids for packaging your AAV product..., species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI Do you have...

  8. Microbiology Resources

    Type
    Collection
    ...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial fields,...and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz...MultiSite Gateway Kit : Creating three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous...Vaccinia virus ORF library : Expression library that contains each of the predicted open reading frames for ... : 285 vectors for expression, markers, fusion proteins, etc - Lindquist Lab Synthetic Biology BIOFAB ...Restoration of prototrophy in common S. cerevisiae lab strains - Ralser Lab Return to top Resources for...

  9. The 12 Days of CRISPR

    Type
    Blog Post
    ...CRISPR, Addgene gave to me: Drag-and-drop genome insertion, without DNA cleavage, with CRISPR-directed integrases...

  11. Viral Vectors

    Type
    Collection
    ...depending on the insert type and envelope used. Please consult with your institution’s Biosafety Committee...that they no longer encode for more virus, but instead encode for a specific gene or other research interest...with specific genomes (Fig. 1A) . In other words, instead of a virus infecting a host and giving rise to ... same way that naturally occurring viruses do. Instead, the genes that are required for viral replication... be produced. Each of these plasmids, however, retains the properties of any typical plasmid and thus ...

  12. The Twelve Days of CRISPR

    Type
    Blog Post
    ...day of CRISPR, Addgene gave to me: anti-CRISPR proteins!  On the ninth day of CRISPR, Addgene gave to ...

  14. Addgene at SfN23!

    Type
    Blog Post
    ...also be sharing our conference experience on X, Instagram, and Bluesky.   Finally, if you're looking to ...
Showing: 841 - 860 of 1041 results