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Showing: 841 - 860 of 1076 results
  1. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    Blog Post
    ..., they fail to account for the effects of Cas9-induced dsDNA breaks. Are off-target cutting patterns in...hypersensitivity, or are in an enhancer, transcription factor binding site, or a coding region. Targeting these sites...Check out this guide to pooled plasmid libraries Find all of the CRISPR Pooled Libraries currently available...
  2. Antibiotic Resistance: An Old Solution but a New Problem

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    Blog Post
    ...for a bacterial infection. While this post will, indeed, focus on bacteria, it is important to note that...resistance genes from it’s environment (from DNA left behind after another bacterium died for instance). These...Are Used in the Lab Probe Phage Genomes for Host Binding Proteins Learn How to Use Our Microbiology Pages...
  3. Tips for Getting a Faculty Position

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    Blog Post
    ...to email me at [email protected]." You can find his original blog post below! --Ed.  Eight years...fundamental to development. I'm interested in how cells find their way to the correct positions. What is the... fit into a department and bring in grants. What kind of colleague will you be? Do you understand what...
  4. Five Popular Model Organisms, Part 2

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    Blog Post
    ...plasmids for use in CRISPR and Tol2 transposase induced transgenesis.  Xenopus Xenopus, or frogs, are one...Addgene.org: Browse our plasmid collection page Find more plasmids and resources for plant resources ...Collection Page Read our molecular biology reference Find plasmids for your research ...
  5. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post
    ...hydrogen bonds and a base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined ...conditions to be screened per plate. The results indicated that the optimal buffer for Taq DNA Ligase contains...DNA ligase I completely encircles and partially unwinds nicked DNA." nature 432.7016 (2004): 473. PubMed...
  6. Antibodies 101: Designing Your First Flow Panel

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    Blog Post
    ...it is also important to know the brightness of individual fluorophores. Table 1 describes the brightness... conjugated to. Since CD45 is a common marker, finding an antibody already conjugated to my fluorophore... In this post, you learned about the principles behind fluorophores and their colors for used in flow ...
  7. Viral Vectors 101 eBook 2nd Edition

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    Blog Post
    ... for bench scientists everywhere. In it, you can find an introduction to viral vectors and their elements... in a clean and easy-to-navigate design. You can find it, along with other eBooks in our collection, on...
  8. Addgene's 20th Anniversary Party

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    Blog Post
    ...and more. It's this diverse group of talented individuals that makes Addgene — well, Addgene. We're so ...delicious food, or playing one of the giant games or indoor mini-golf course.    While the main purpose of ...
  9. Twenty Years of Sharing Science!

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    Blog Post
    ...partnered with distributors in China, Korea, Japan, India, and Brazil.  We've also created a robust support...us the honor of being part of yours. We hope you find our services helpful in your scientific pursuits...
  10. Expanded Access to Rabies Plasmids

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    Blog Post
    ...under this application process since May 2020. We find this process takes 4-6 weeks on average. Therefore...licenses is available through the US Bureau of Industry and Security. If you have any questions about ...
  11. DIY DNA Ladders from Penn State University

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    Blog Post
    ...interested in making your own DNA ladder? You can find the Tan Lab’s DNA ladder prep protocol here and ...Frugal Scientist? Save Money with the Baby Gel Box Find 5 Smart Phone Apps for Lab Life Learn About the ...
  12. New Educational Resource: The Addgene Videos Page

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    Blog Post
    ...resource for scientists around the world. With that in mind, we are very excited to debut our brand new Addgene...goal of this page is to make it easier for you to find content relevant to your research and career goals...
  13. Bringing Renewable Energy to Addgene

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    Blog Post
    ...in New England- the majority of which are solar, wind, or hydroelectric in nature. The operation of -80C...other scientists save resources by sharing plasmids Find plasmids at Addgene ...
  14. CRISPR Cheat Sheet

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    Blog Post
    ... Sheet Clustered Regularly Interspaced Short Palindromic Repeats: Sequences of DNA found in bacteria that...DNA or RNA sequences. Nucleases: Proteins that bind to gRNAs and are directed by the gRNAs to cut particular...
  15. New Podcast Segment: Hot Plasmids

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    Blog Post
    ...website. As we push forward with these efforts, you’ll find new ways to learn about science careers, lab protocols...Plasmid articles with each of our newsletters. You can find all of these articles on the Hot Plasmids webpage...
  16. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

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    Blog Post
    ...Explains His Path from Grad Student to Entrepeneur Find Resources to Help You Choose Your Career Path Resources...on Addgene.org Check Out Our CRISPR Guide Pages Find Ready-to-Use CRISPR Lentivirus Learn about Other...
  17. Countdown to Halloween @Addgene

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    Blog Post
    ...though I suspect that our costume contests were indeed occurring earlier but that the photo evidence was...costumes this Friday. So if you’re interested in finding out who wins this year’s Addgene costume contest...
  18. AAV Packaged on Request is Here!

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    Blog Post
    ...Packaged on Request With Packaged on Request, you can find your plasmids and your viral packaging service in...always available at [email protected].  We hope you find this service useful in your research. Happy vectoring...
Showing: 841 - 860 of 1076 results