Addgene

We narrowed to 908 results for: IRE;

Showing: 841 - 860 of 908 results

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Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Type

Blog Post

...-CRISPRi is stably expressed with no selection required and titratable knockdown allows for the study ...
Viral Vectors 101: Producing Your rAAV

Type

Blog Post

...however, is a “helper-free” system, in which the required adenoviral helper genes are supplied on two different...
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

...hundreds to thousands of different pegRNAs for each desired edit, it simply becomes impossible (or at least...
Twenty Years of Addgene Sharing: CRISPR

Type

Blog Post

... blog posts are also frequently read: Homology Directed Repair has been viewed 219,289 times, while Validating...
PCR Applications for Copy Number Variation Assays

Type

Blog Post

...accurately read (Bell, 2018.) Even though it does not require a standard curve or a normal control, it can take...
Transferable Skills: Negotiation

Type

Blog Post

...resolution. Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate...
Tips for Using BLAST to Verify Plasmids

Type

Blog Post

...alternatives Depending on your sequencing result and desired analysis, BLAST may not always be your optimal ...
Great Results Start with Great Standard Curves

Type

Blog Post

...software, don’t give up! Rather than plotting the entire data set, plot only the linear portion. This allows...
Uncovering Ancestral Bias in CRISPR Research

Type

Blog Post

...racial bias from work being done today, is a nearly entirely separate effort from acknowledging its existence...
A Guide to Starting Your Own Journal Club

Type

Blog Post

...And, many wanted to learn more, even those not directly involved in the project. Once we gauged interest...
Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

... to help the DNA enter the cells. Some samples require a reduced antibiotic concentration or need to be...
Plasmids 101: Protein tags

Type

Blog Post

...cytometry, but rarely used for purification as elution requires low pH poly His HHHHHH 0.8 Affinity and Purification...
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Type

Blog Post

...pre-set timers for incubation steps, and save the entire process so you’ll have a record of your work. This...
Negotiating Work and Life: How to Find the Joy

Type

Blog Post

...that charming fixer-upper house you have always admired or volunteer to lead a charitable cause. While...
How to Write a Scientific Review Article

Type

Blog Post

...convert the citation style to whatever the journal requires. Using the (Last name, Year) format also has the...
Deciphering the Mysteries of Behavior with Viral Vectors

Type

Blog Post

... addition to the neuronal networks involved in directly controlling muscle activity and coordinating movement...
Twenty Years of Sharing: Addgene's Viral Vector Service

Type

Blog Post

.... The supporting data and community engagement required to do this drove Addgene to develop new systems...
An Inside Look into Addgene's Dev Team

Type

Blog Post

...lines of showing that the checkout takes the user directly to the shipping step, and no longer takes the ...
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Type

Blog Post

...the production of a cell surface glycan that’s required for ricin uptake. The ricin screen did fail to...
Is this the right place for me? 8 tactics for choosing a lab

Type

Blog Post

...Your mental health and success in science will directly depend on your science mentor. One scientist who...

Showing: 841 - 860 of 908 results

Results per page:

Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Viral Vectors 101: Producing Your rAAV

PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Twenty Years of Addgene Sharing: CRISPR

PCR Applications for Copy Number Variation Assays

Transferable Skills: Negotiation

Tips for Using BLAST to Verify Plasmids

Great Results Start with Great Standard Curves

Uncovering Ancestral Bias in CRISPR Research

A Guide to Starting Your Own Journal Club

Tips from the Repository Trenches: Using Barcodes to Track Samples

Plasmids 101: Protein tags

Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Negotiating Work and Life: How to Find the Joy

How to Write a Scientific Review Article

Deciphering the Mysteries of Behavior with Viral Vectors

Twenty Years of Sharing: Addgene's Viral Vector Service

An Inside Look into Addgene's Dev Team

Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Is this the right place for me? 8 tactics for choosing a lab

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