We narrowed to 916 results for: IRE;

1. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   ...perform differently over time), compatible with the desired applications (such as immunohistochemistry (IHC...

2. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   ...al., 2019). Finally, scientists can perform the entire process of antibody selection and maturation in...

3. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   ...GFP.   Figure 2: Synthetic promoters can be paired for targeted expression. This same pairing of...

4. Early Career Researcher Toolbox: Finding Relevant Papers

   Type

   Blog Post

   ...This is a great way to have new papers delivered directly to your inbox. Best uses Staying up-to-date on...

5. Tips for Technical Support Calls

   Type

   Blog Post

   ...end of that email you’re writing is probably not directly responsible for the problem. We’re here to help...

6. Illuminating Epigenetics with A FRET Based Biosensor

   Type

   Blog Post

   ... has recently been hitting the headlines, with sotires like the potential devastation of the palm oil ...

7. Experimenting in an Open Source lab: from CRISPR to Cats

   Type

   Blog Post

   ...probably never use the Illumina GAIIx we recently acquired  for sequencing again - it’s too inefficient and...

8. The Scientific Conference Poster Session: Tips for Success

   Type

   Blog Post

   ...gone up to a poster where the presenter spends the entire time talking to the poster, as opposed to engaging...

9. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   ...-CRISPRi is stably expressed with no selection required and titratable knockdown allows for the study ...

10. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    ...however, is a “helper-free” system, in which the required adenoviral helper genes are supplied on two different...

11. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    ...hundreds to thousands of different pegRNAs for each desired edit, it simply becomes impossible (or at least...

12. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    ... blog posts are also frequently read: Homology Directed Repair has been viewed 219,289 times, while Validating...

13. PCR Applications for Copy Number Variation Assays

    Type

    Blog Post

    ...accurately read (Bell, 2018.) Even though it does not require a standard curve or a normal control, it can take...

14. Transferable Skills: Negotiation

    Type

    Blog Post

    ...resolution. Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate...

15. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    ...alternatives Depending on your sequencing result and desired analysis, BLAST may not always be your optimal ...

16. Great Results Start with Great Standard Curves

    Type

    Blog Post

    ...software, don’t give up! Rather than plotting the entire data set, plot only the linear portion. This allows...

17. Uncovering Ancestral Bias in CRISPR Research

    Type

    Blog Post

    ...racial bias from work being done today, is a nearly entirely separate effort from acknowledging its existence...

18. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    ...And, many wanted to learn more, even those not directly involved in the project.  Once we gauged interest...

19. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    ... to help the DNA enter the cells. Some samples require a reduced antibiotic concentration or need to be...

20. Plasmids 101: Protein tags

    Type

    Blog Post

    ...cytometry, but rarely used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification...