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TypeBlog Post.... Dashboards provide information in a concise and clear way that is accessible by a wide audience. The...parse through, aggregation of this information can provide valuable insight to the most requested plasmid,...
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Newly Updated AAV Data Hub!
TypeBlog Post...too! Using the Data Hub If you're trying to decide which AAV is right for your experiment, searching.... You can check out our step-by-step submission guide or email [email protected] to learn more. A great..., when published, will meet FAIR data-sharing guidelines. But what if my AAVs didn't work? We love negative... -
Plasmid Grant to Aid Cervical Cancer Screening in Ghana
TypeBlog Post... represents the continuation of our efforts to provide resources to researchers in developing countries...Ghanaian women by including PCR screening for HR HPV alongside the cytological tests currently in use. Gladys...screens. She hopes that these screens will both identify individuals who are infected with HR HPV and therefore... -
Addgene’s Blog Gets a New Look!
TypeBlog Post...share scientific discoveries and developments, and provide tips and tricks for new and seasoned molecular ...specific area of interest. We also cover topics outside of these five areas and you can find what you need...comments below. We’ll track down the story, get the inside scoop, and keep you up-to-date. Be the first to... -
The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing
TypeBlog Post...Studies Hybridoma Bank. Antibodies are the most widely used class of protein-binding reagents. In the ...and expanded. The clonal cells are screened to identify hybridoma lines, each producing a monoclonal antibody...ensuring affordable access to materials, repositories provide standardized quality-control and technical support... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog Post...-one CRISPR/Cas9 vectors expressing up to seven guide RNAs (gRNAs). Sakuma said that the new system could...likely to work in animal embryos based on earlier evidence showing that a CRISPR/Cas9 plasmid could be injected...vector-mediated multiplex genome engineering. The system now provides an efficient targeting strategy for multiplex ... -
The Addgene Antibody Data Hub
TypeBlog Post...citation. If you’re considering requesting an Addgene antibody, the Data Hub can help you decide if an antibody... your application. It can be quite difficult to decide on which antibody to request for your experiment... -
Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6
TypeBlog Post...student in the Collins' lab at the time) got the idea to engineer bacteriophage (viruses that infect bacteria...bacteria) for use as antibiotics. To develop this idea, Drs. Koeris and Lu founded the biotech startup ... as antibiotics 8:26 - 10:30 : How Dr. Koeris' decided to develop phage for use in a biotech startup 12... -
MassBioEd Career Exploration Day at Addgene
TypeBlog Post...visited our labs in Cambridge, MA to learn about the wide variety of science careers that Addgenies represent...travels from 2015, including a great European photo slideshow from our UK team member Benoit Giquel. Bluegene...Regional High School Resources at Addgene Read more Inside Addgene Blog Posts Get Career Advice from Our Bloggers... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog Post...the DNA sequencing reaction should be repeated. Guidelines to help with DNA sequencing troubleshooting and... might suspect contamination in your sample or consider asking your sequencing facility to apply a special...vendors. The precipitation method has an unfortunate side effect of messing up the reaction around base 70... -
Microbes: Look, Listen, and Tell
TypeBlog Post...Stanford University). Right there on the floor outside the lecture hall. It was wonderful to see the ad-hoc...acoustic biomolecule-based dyes. This technology is widely applicable as bacterial expression of dyes allows...Microbial World’ showcased complex connections and ideas in various visualized approaches from hand-drawn... -
Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog Post...committee creates a compensation philosophy that helps guide employee compensation and also serves as an objective...think is the most important resource that Addgene provides the scientific community? Access to plasmids which...Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog... -
Our Most Popular Blog Posts from 2019
TypeBlog Post...on the Addgene blog and have created and updated guides on various topics in biology over the past year...strategies for generating GFP fusion proteins. From considering protein size and shape, linker sequences, and...Cas9 and reverse transcriptase and a prime editing guide RNA, it can introduce insertions, deletions, and... -
Editor's Choice, August 2016
TypeBlog Post...Pooran surveyed a CRISPR google forum to get a better idea of how many people are having trouble, to determine...from Cornell University, is a bit old school and provides a thorough walkthrough of how you can use PCR ...Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science Talk Developing Lab... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog Post...treatment, it is best to identify a cocktail of 3-12 phages. Once these phages are identified, they must then ... forward to help, the Phage Directory team must decide which labs should receive the clinical sample to... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post...high-throughput manner, but these systems have not been widely employed in vivo. The CHIME and new X-CHIME systems...deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo gene knockout in immune systems...CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions in Mammalian... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog Post...may have only 2-3 formal supervisors that will provide guidance and experience. Navigating a fulfilling...about mentoring In this blog series, I plan to provide answers to the top questions scientists ask me ...teaches you to fish, doesn’t fish for you) Helps identify a Mentee’s strengths and make the most of them... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog Post...Protein Collection webpage. These plasmids are divided into the following categories: Over 300 empty ...localizes in the cell, use these markers as your guide. As Michael Davidson told the Tallahassee Democrat...go together, we can fix it. Right now, we have no idea." Now, it will be that much easier for scientists... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...reporters that are detectable by an inexpensive and widely available imaging platform: ultrasound. Challenges...experiments because ultrasound devices are inexpensive, widely available, and have deep tissue penetration and... imaging. Specifically, arg1 expressing E. coli aided in ultrasound imaging of the mouse gastrointestinal... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog Post...fact, there was so much useful information that I decided to write a few blog posts. This first post will...the box so the pre-paid postage stamp is on the outside of the container, and drop off the box at any location...sustainability leaders. Take initiative to bring ideas to your workplace and you could end up making a ...