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... has also used CellTags for tagging two cell populations in a competitive transplant assay in the mouse...

...Addgene is thrilled to announce the launch of our latest community resource: Recombinant Antibodies! In ...

...DOI: 10.1038/521274a Arribere, J. A., et al., Translation readthrough mitigation. Nature. (2016) 534, 719...

...and encoded on a plasmid, they can be easily manipulated into more useful tools. In the case of isotype...

...describes how you can better design your donor DNA template to achieve more efficient genome editing - all...

...Whether you’d like to knockout a gene, replace it, modulate its expression, or perform a genome-wide screen...

...jargon and key phrases and understand enough to relate it to the position I was applying for. After all...

...lymphocytes. Griesbeck and his colleagues began by isolating a calcium-binding protein, troponin C (TnC), from...

...virtually means getting to know the app or virtual platform and making sure you stay connected. If possible...

...and Facebook. Find more COVID-19 and coronavirus related plasmids and resources at Addgene* Many thanks...

...gram negative. A German scientist, Carl Weigert, later expanded on Gram’s discovery, adding a second stain...

... a top choice for multiplex gene knockout or regulations, it lacks proper functions to enable efficient...

... finding and organizing papers, check out this related blog post: Early Career Researcher Toolbox: Finding...

...use a non-specific endonuclease (Cas9 or closely related Cpf1) to cut the genome and a small RNA (gRNA) ...

...and this is a recipe for bad outcomes. Clearly articulate the desired outcomes. Begin with the end in mind...

..., in principle, these experiments could find correlations between variants in a gene of poorly classified...

...CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR...

... it.  In the lab, it might mean persuasively articulating why the results you've measured should change...

...a talk about the different parts of the brain stimulated by stories. In retrospect, I should have expected...

... they can be made competent through chemical manipulation in the lab. This is commonly done using calcium...