We narrowed to 1,015 results for: NTS;

... is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a has generally been underutilized...low-pressure teaching opportunity for lab members or students new to protein expression and purification. ...

...antibodies through cells and into different body compartments, such as into mucus, tears, or milk. When using...switches to producing IgE antibodies, the gene segments for IgM, IgD, and IgG are deleted.     ...

...just getting started using antibodies in your experiments, you may be curious about all of the different... are first injected with a small peptide that represents a short, unique amino acid sequence found in ...

... into the key takeaway themes and customer pain points we’ve identified. I can’t wait to send it out. ...work on next to help alleviate any customer pain points. Many thanks to our guest blogger Rachel Rubinstein...

...have to weed through fewer colonies after your experiments. As a common example, a selection will leave ...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-...

... how to use viruses to set up these systems. Components of a biosensor Some of the biosensors used in...transducer will generate a numerical value which represents the amount of analyte present in the system at...

... Unlike traditional dye-based neuronal tracing reagents, AAV particles require receptor-mediated entry...neurons. When using this serotype to conduct experiments with chemogenetic vectors, this property must...

...cancer and defeat it we need to attack it from all fronts!”  Here I aim to compile a few highlights from ...favorable state for tumor progression (Ref 2). Future treatments may need to target this immunosuppressive reaction...

...transduction with this virus. While various Cas9 mutants have been engineered to perform unique editing ...consistent levels of Cas9 expression between experiments and over time (as compared to separate transductions...

...and SLIC can all be used to assemble multiple fragments, but these methods can be expensive because they...and frozen in large batches suitable for many experiments. When REPLACR-mutagenesis goes head-to-head with...

... of endogenous genes and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion...considering the use of pooled libraries in your experiments, Addgene recommends that you review the steps...

...control in many genetic targeting and editing experiments. Find the CASANOVA plasmids here! Bubeck F et...for protein-protein interaction (PPI) studies in plants that rely on transient transfection systems using...

...increasingly seeking us out on their own to share reagents. I love the way scientists starting their own ...scientists who request from us but also by high school students and industry scientists as a universal resource...

...genome engineering. Planarian worms cut into three segments “remember” which side should grow a head and a...bioelectric signaling networks: exploiting voltage gradients for control of growth and form." Annual review...

...not cancer cell populations resistant to drug treatments arise during treatment or are preexisting in ...really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re excited to see the creative...

...control spatially as to what’s happening at different points within the wood. There are different types of cellulose...hundreds of genes. We’ve created enough regulatory elements, CRISPRs, repressors, activators. We’ve been putting...

...Antibodies are used in many different experiments that require scientists to detect proteins in their...visualization. The indirect ELISA is used for similar experiments as the direct ELISA, the only difference being...

...-species staining protocols that use antibody fragments to block the endogenous immunoglobulins and reduce...complex with a variety of proteins interacting. Experiments tend to be equally complex with users trying ...

... best strategies and protocols for different experiments.  In this blog post let's take a look at some...lab in 2015 adapted the AID system discovered in plants to conditionally deplete proteins of interest in...

...phenotype and helps improve the reproducibility of experiments. Inbred mice are particularly useful for understanding...recessive traits and their impact on diseases or treatments, but are not useful for genetic engineering. ...