We narrowed to 1,020 results for: NTS;
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TypeBlog Post...babies,” where fused genetic material from intended parents is “rehoused” in a separate, enucleated zygote ..., despite many headlines questioning if He’s experiments prove otherwise, the scientific process does ...
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Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...type of bacteria it is being used in, and how components of the plasmid should change based on bacteria...leading to safer medicine and more successful experiments. It’s something that all scientists should understand... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...supervisory experience working with undergraduate students? On top of that, perhaps you were involved in ...officers working specifically to assist graduate students in job searching. Start your career planning early... -
Viral Vectors 101: Biosensors
TypeBlog Post... how to use viruses to set up these systems. Components of a biosensor Some of the biosensors used in...transducer will generate a numerical value which represents the amount of analyte present in the system at... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post... Unlike traditional dye-based neuronal tracing reagents, AAV particles require receptor-mediated entry...neurons. When using this serotype to conduct experiments with chemogenetic vectors, this property must... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...cancer and defeat it we need to attack it from all fronts!” Here I aim to compile a few highlights from ...favorable state for tumor progression (Ref 2). Future treatments may need to target this immunosuppressive reaction... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...transduction with this virus. While various Cas9 mutants have been engineered to perform unique editing ...consistent levels of Cas9 expression between experiments and over time (as compared to separate transductions... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog Post...have to weed through fewer colonies after your experiments. As a common example, a selection will leave ...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-... -
AAV Q&A with Tim Miles
TypeBlog Post...diversity in Vector Assisted Spectral Tracing (VAST) experiments using PHP.eB in the brain give some insight into...Xinhong Chen, and Miggy Chuapoco, and to all our participants for their time and excellent questions. ... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...validation pipeline. (B) Schematic shows the separate elements of the R-mAb expression plasmid involved in coexpression...R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue to be available... -
Hot Plasmids - February 2022
TypeBlog Post... is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a has generally been underutilized...low-pressure teaching opportunity for lab members or students new to protein expression and purification. ... -
Antibodies 101: Isotypes
TypeBlog Post...antibodies through cells and into different body compartments, such as into mucus, tears, or milk. When using...switches to producing IgE antibodies, the gene segments for IgM, IgD, and IgG are deleted. ... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post...just getting started using antibodies in your experiments, you may be curious about all of the different... are first injected with a small peptide that represents a short, unique amino acid sequence found in ... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post... into the key takeaway themes and customer pain points we’ve identified. I can’t wait to send it out. ...work on next to help alleviate any customer pain points. Many thanks to our guest blogger Rachel Rubinstein... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...and SLIC can all be used to assemble multiple fragments, but these methods can be expensive because they...and frozen in large batches suitable for many experiments. When REPLACR-mutagenesis goes head-to-head with... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post... of endogenous genes and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion...considering the use of pooled libraries in your experiments, Addgene recommends that you review the steps... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...control in many genetic targeting and editing experiments. Find the CASANOVA plasmids here! Bubeck F et...for protein-protein interaction (PPI) studies in plants that rely on transient transfection systems using... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...increasingly seeking us out on their own to share reagents. I love the way scientists starting their own ...scientists who request from us but also by high school students and industry scientists as a universal resource... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...genome engineering. Planarian worms cut into three segments “remember” which side should grow a head and a...bioelectric signaling networks: exploiting voltage gradients for control of growth and form." Annual review... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...not cancer cell populations resistant to drug treatments arise during treatment or are preexisting in ...really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re excited to see the creative...