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We narrowed to 1,010 results for: PLE

Showing: 841 - 860 of 1010 results

  2. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...several different species are present. One common example is using pre-adsorbed secondary antibodies (which... other antibodies) in experiments that require multiple antibodies that each detect a different antigen...

  3. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific... observation of individual endogenous protein complexes in situ by proximity ligation." Nature methods...

  4. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...deletion in MYBPC3 donated skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and...Read about High Fidelity CRISPR Variants Target Multiple Genes at Once with CRISPR Resources on Addgene.org...

  5. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... for photoswitchable kinases.  Below are a few examples demonstrated in Zhou et al. 1. Testing kinase ...Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in Live Single Cells." Cell ...

  7. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ... not all grant duties to trusted students. For example, if the proposal call aligns with your work, your...Below are the typical sections in a NIH RFA, for example, and tips to consider while writing each section...

  9. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...treatments, but are not useful for genetic engineering. Examples of outbred strains include Swiss Webster and Harlan...female mouse for gestation. All three can introduce multiple base pair deletions, but CRISPR/Cas9 can also ...

  10. Engaging with science and society at pgEd

    Type
    Blog Post
    ...of science, even while I was a bench scientist completing my graduate studies in molecular biology and ...academic writing and presentations. But for many people who don’t work in these fields of research, what...

  11. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...or be detected even if the two proteins form a complex. To help troubleshoot your experimental setup ...Overcoming current limitations to permit the use of multiple FRET biosensors simultaneously would further increase...

  12. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...varies widely with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First...

  14. Of Myc and Men

    Type
    Blog Post
    ...specific epitope that your antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le...be the antibody Mary Poppins!   All of the examples of new versions of Myc1-9E10 mentioned above basically...

  15. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...known tropism for your cells of interest — for example, AAV8 for kidney cells — can help improve infection... away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1). Even...

  16. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...functions in use But exactly what kind of editing are people doing with all these CRISPR plasmids (and libraries...these categories to see what kinds of CRISPR tools people were depositing and requesting each year (Figure...

  18. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...PAM or sgRNA sequences Cas9 likely goes through multiple rounds of cleavage at the target site until either...that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is...

  19. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...
Showing: 841 - 860 of 1010 results