We narrowed to 921 results for: Por

1. Plasmids 101: Blue-white Screening

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   ...they should be. Don't rush the process: It is important to give your plates enough time for any intact...

2. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ...research. But be aware that the fluorescent proteins reported in publications are only characterized under a...

3. CRISPRainbow and Genome Visualization

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   ...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA...

4. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ...understanding all of the players becomes ever more important to ensure that you choose the most appropriate...

5. RNA Extraction Without A Kit

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   ...extractions- if you need really clean RNA, it’s important to make sure that when extracting, to only take...

6. Summer SciComm Series: Cognitive Load

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   ... too little)?  The answer is: it depends! The important point here is to pay attention to exactly what...

7. Antibodies 101: Secondary Antibodies

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   ...subclasses, such as mouse IgG2 or IgG4. This can be important for some applications. Assay use While secondary...

8. What's New in CRISPR - November 2021

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   ...making it well suited for AAV packaging. Most importantly, this CasMINI system was shown to be effective...

9. Antibodies 101: Conjugation

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   ...find yourself doing your own conjugation, it is important to keep in mind that you will need to confirm ...

10. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    ...into a hole in their cage. Since dopamine is an important neurotransmitter that promotes reward-motivated...

11. Developing Lab Management Software for Biology

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    ...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true...

12. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    ... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...

13. New Tool for Lineage Tracing: The ClonTracer Library

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    ...leukemia. Bhang says the work highlighted the importance of developing more potent ABL1 allosteric inhibitors...

14. Plasmids 101: Golden Gate Cloning

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    ...like Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs...

15. Plasmids 101: Cre-lox

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    ... Plasmids 101 series, we examined a number of important plasmid elements – promoters, origins of replication...

16. Adenoviral Vector Production and Troubleshooting

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    ... quickly due to immune response against AdV. Importantly, recombination-competent adenovirus (RCA) can...

17. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...Hybridization (WISH) WISH is a technique that detects the temporal and spatial localization of mRNA transcripts in...

18. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...while also capturing information about spatial and temporal organization. The design use to detect PKA activity...

19. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...basic research and other ongoing clinical trials support the utility of AAV as a broadly applicable gene...

20. Plasmids 101: The Wide World of Natural Plasmids

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    ...plasmids — more on that in a minute! Fun fact! Electroporation is one popular method scientists use to promote...