We narrowed to 928 results for: Por

1. Antibodies 101: Buffers, Storage, and Conjugates

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   Blog Post

   ...whenever you use the antibody. This information is important, and you’ll need to track it throughout your experiments...

2. 15 Years of Addgene: The Top 15 Plasmids

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   Blog Post

   ...requestors for making their reagents available and for supporting scientific sharing. We’re very excited for more...

3. Plasmids 101: Methylation and Restriction Enzymes

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   Blog Post

   ... directed mutagenesis. During this process, incorporation of a desired mutation into your plasmid of interest...

4. How to Keep a Lab Notebook for Bioinformatic Analyses

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   Blog Post

   ...your methods section about the edit that was temporarily used. Just be sure that the original code remains...

5. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   ...the deletion. REPLACR-mutagenesis successfully incorporated a 60-nucleotide insertion; beyond this insertion...

6. Plasmids 101: Blue-white Screening

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   ...they should be. Don't rush the process: It is important to give your plates enough time for any intact...

7. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   Blog Post

   ...research. But be aware that the fluorescent proteins reported in publications are only characterized under a...

8. CRISPRainbow and Genome Visualization

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   Blog Post

   ...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA...

9. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   Blog Post

   ...understanding all of the players becomes ever more important to ensure that you choose the most appropriate...

10. Summer SciComm Series: Cognitive Load

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    Blog Post

    ... too little)?  The answer is: it depends! The important point here is to pay attention to exactly what...

11. Antibodies 101: Secondary Antibodies

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    ...subclasses, such as mouse IgG2 or IgG4. This can be important for some applications. Assay use While secondary...

12. What's New in CRISPR - November 2021

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    Blog Post

    ...making it well suited for AAV packaging. Most importantly, this CasMINI system was shown to be effective...

13. RNA Extraction Without A Kit

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    Blog Post

    ...extractions- if you need really clean RNA, it’s important to make sure that when extracting, to only take...

14. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ...into a hole in their cage. Since dopamine is an important neurotransmitter that promotes reward-motivated...

15. Antibodies 101: Conjugation

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    ...find yourself doing your own conjugation, it is important to keep in mind that you will need to confirm ...

16. Developing Lab Management Software for Biology

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    Blog Post

    ...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true...

17. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    ... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...

18. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post

    ...leukemia. Bhang says the work highlighted the importance of developing more potent ABL1 allosteric inhibitors...

19. Plasmids 101: Golden Gate Cloning

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    Blog Post

    ...like Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs...

20. Plasmids 101: Cre-lox

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    Blog Post

    ... Plasmids 101 series, we examined a number of important plasmid elements – promoters, origins of replication...