Skip to main content
Addgene
Showing: 841 - 860 of 1011 results
  1. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...Ready to start epitope tagging?   References and Resources References Terpe, K., Overview of tag protein...
  2. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...more efficient and feasible for many labs.  References Efficient Intracellular Delivery of Native ...into all cells? Schwarze SR, Hruska KA, Dowdy SF. Trends Cell Biol. 2000 Jul;10(7):290-5. PubMed. Is VP22...
  3. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...enable observation of the interaction between different molecules like zinc and calcium. Ultimately, Merkx... start using it. For us, it’s like a test.” References Thestrup et al. Optimized ratiometric calcium...
  4. Addgene's eBook Collection

    Type
    Guide
    ...you're not sure where to start or need a handy reference, Addgene's eBooks are here to help!...you're not sure where to start or need a handy reference, Addgene's eBooks are here to help! 0 of 0 Selected...
  5. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule so you can rely ...Most Micropublications contain one figure. If you aren’t there yet, write introductions to potential publications...
  6. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...multiple samples without cross contamination. We currently use the CosMCPrep system for this, but thought... because it forced workshop participants from different biotech companies to interact and learn more about...
  7. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...provides something that you can’t obtain at your current institute. A common reason is hands-on training... right for you, start thinking outside of your current research box, send out some feelers, apply to programs...
  8. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP Monoclonal...
  9. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ...
  10. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...permeable to CO2 at low temperatures. We tested two different types of tubes: the ones we use to store lentivirus...also using the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer...
  11. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...the interview process was several short talks by current faculty members and it just blew me away….  I have...overall mission of making science easier and more transparent. That’s largely my job [at the Broad] too… to...
  12. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...interests, and spends one-on-one time with the current members to share short-term goals and chat about..., but even brief stays there can make all the difference. Choose a lab that brings on the happy dance ...
  13. Hot Plasmids - May 2022

    Type
    Blog Post
    ...peptide. By combining three different localization peptides with four different fluorescent proteins, and...
  14. Antibodies 101: ChIP

    Type
    Blog Post
    ...optimized for each specific sample type. And, if that weren’t bad enough, optimization of one step can affect...protocols when embarking on your ChIP journey!  References and Resources More resources on Addgene’s blog...
  15. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...illustrates the utility these tools have in many different areas of biology as they grow. The top 15 plasmids...envelope protein enables this virus to infect many different cell types so it is useful in a wide range of ...
Showing: 841 - 860 of 1011 results