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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,006 results for: RING

Showing: 841 - 860 of 1006 results

  1. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...If the fall colors are inspiring you to paint your cells in equally vivid colors, you'll want to check...

  6. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...scaffold. gRNA: "Guide RNA" is a general term referring to the RNAs used by Cas proteins, either a crRNA...

  13. Molecular Biology Reference

    Type
    Guide
    ...bacteria, which can uptake plasmids and amplify them during cell division. Because bacteria are easy to grow...that can drive expression of short RNAs. Genome Engineering Plasmids - Used to target and edit genomes. Genome...of animals. There are many different naturally occurring strains of E. coli , some of which are deadly ...Carbenicillin can be used in place of ampicillin. Preparing Antibiotics Create a stock solution of your antibiotic...Addgene recommends making 1000X stock solutions and storing aliquots at -20°C. To use, dilute your antibiotic...Specifically, A pairs with T and C pairs with G. During replication, DNA unwinds and the DNA polymerase...utilizes modified fluorescently tagged nucleotides. During illumina NGS, a long piece of DNA is first fragmented...

  14. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...ideal with Myc, as low pH conditions are required during the purification, which may affect protein function...

  19. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...electro-competent E. coli - excess salt causes arcing during electroporation which would cause the electroporation...

  20. Plasmid Cloning by PCR

    Type
    Blog Post
    ...of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for cloning Run the PCR reaction...
Showing: 841 - 860 of 1006 results