We narrowed to 982 results for: Ran;

...Addgene blog Read all careers blog posts Read our transferable skills guide Resources on Addgene.org Find...

...scientists and the public and help make science more transparent. Second, SITN helps graduate students develop...

...rapidly in 2011, from zinc finger nucleases to transcription activator-like effector nucleases (TALENs). ...

...label neurons with mCherry and you already have a transgenic mouse line that contains a Cre-dependent mCherry...

...end with little more than an Excel spreadsheet of random numbers that’ll leave you, well, #sad. Learn...

...Different brands and FBS lots can promote or inhibit transfection. Test a variety of brands and FBS lot...-term protein expression observed using transient transfection approaches, generating cell lines using...experiments in a stable cell line, as opposed to transiently-transfected cells, increases reproducibility, as it... the variation associated with repeated transient transfection. Some lentiviral vectors deliver mammalian...stable cell culture after transduction. Performing antibiotic selection on transduced cells enables elimination...Pro-Tip Transducing too many cells relative to the number of virus particles reduces the transduction efficiency... may have 1 copy of the transgene, while others have >1 copy of the transgene in their genome. This can...

...disease. This year, Addgene will be providing plasmid grants worth $2,000 each to two labs through the BeHEARD...

...generating variants with improved affinity and dynamic range of both nLightG and nLightR, and may soon have a...

... the entire FAQ collection. You’ll find topics ranging from depositing your lab’s materials and requesting...

...practices of indirect costs.  The peer review and grant awards systems need to be radically overhauled.  ...

...this type of experimentation to the eventual translation to the clinic and cures. There is no doubt that...

...It is clear that one needs to capture a diverse range of CTCs to cope with the variation in cancer, not...

...target cells that have previously been stably transfected for Cas9 expression. The Yusa lab has used their...

...Gel Transfer Device. Wet the pre-run gel in deionized water and place it on the transfer membrane of the...protocol uses a dry transfer device but can be adapted for wet and semi-dry transfer approaches. Last Update...well. Assemble the transfer sandwich as follows: Unseal the iBlot 2 PVDF Mini transfer stack. Set the Top... parameters are correct. Transfer for 5–6 min for proteins <30 kDa. Transfer for 8–10 min for proteins...before running your experiment. Remove the membrane from the transfer device and place it in a container with...Block the membrane in blocking buffer for 1 h at RT on a shaking platform. Wash the membrane 3x for 5 ...Incubate the membrane with secondary antibody for 60 min at RT on a shaking platform. Wash the membrane 3x for...

...expressing ECN is 109 cells/ml, which is within the range of  the ~1011 commensal bacteria per gram of stool...

...be located. By easily using BLAST to check the translated amino acid sequence from within Snapgene, we ...

...preprints, data, software, micropublications) in grant proposals. ReFigures are one such research product...

...measure, here’s a few other posts about developing transferable skills while in grad school Resources on Addgene.org...

...New icons can be requested, although there’s no guarantee Biorender will create it. Additionally, you can...

...materials are both excellent ways to provide transparency, support reproducibility, and accelerate science...