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Showing: 841 - 860 of 1022 results

  1. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...type are generally broken down into two broad categories, prokaryotic or eukaryotic, based on the functional...termination mechanisms fall under two general categories: rho-dependent and rho-independent. Rho factor...

  2. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...particle. Consequently, titers based off of p24 quantification tend to be overestimates since they can include...quantify the amount of PCR product that comes from amplification of the original plasmid which can over-inflate...

  4. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...results. Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red))...Anything between orange and light orange usually indicates that the cells have grown enough, are making their...

  5. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...delegating well, but it does get easier over time! Communicate often and clearly As a manager you need to clearly...it is the best way to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable...

  6. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or...brightness with increasing depth caused by light scattering and absorption.  Finally, the photomultipliers...

  7. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...search to check for expected ORFs, mutations or truncations. A primary advantage of blastx is that you do...other labs speed up their plasmid and cloning verification steps and free up more time for using your plasmids...

  8. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... creates a template that’s suitable for PCR amplification using pre-designed primers. By using this library...morphologic studies), as well as translational applications (mechanistic studies and gene therapy treatments...

  9. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...have made great strides to map brain regions and allocate specific functions to them. Electrical recordings...long-lasting antidepressant effects." Nature communications 10.1 (2019): 223. PubMed PMID: 30644390. PubMed...

  10. Lab to Office Culture Shock

    Type
    Blog Post
    ...involves a few key features that can be difficult to replicate in other work settings: The sense of being the... might include collaborating, managing, and communicating. After years of focusing on my technical skills...

  11. How to Be an Excellent Trainee

    Type
    Blog Post
    ... check out Addgene’s blog, eBooks, and other educational resources to help you get started!) Are there...something. It can be intimidating to ask for clarification at first, but in the end everyone wins. Your...

  12. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...The glycoprotein will be incorporated into the replicating rabies virus envelope, travel retrogradely, and... Pseudotypes: Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene...

  13. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...ribosome may therefore stall at inappropriate locations or fail to make it through the entire transcript...generally segregate with the cell pellet during purification procedures. Even if you produce a large amount...

  14. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... conferences are held every year in different locations all over the world. But simply attending the conference...work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great blog ...

  15. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging.....org Find Cas9 Activators Find Cas9 Repressors Catch Up on Your CRISPR Background with Our CRISPR Guide...

  16. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...allows the detection of molecules' interactions, modifications or dissociations in situ. Used since the mid...14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...

  17. Five Popular Model Organisms

    Type
    Blog Post
    ... throughout their lifetime. Thus any genetic modification, such as expression of fluorescent protein or...studying somatic mutations and cancer." Nature Education 1.1 (2008): 183. Zuber, Johannes, et al. "Mouse...

  20. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...the user. This approach is useful for several applications as will be discussed below. The software could...contain multiple gRNAs targeting all genes in the indicated species and were suggested to be the best gRNAs...
Showing: 841 - 860 of 1022 results