We narrowed to 1,104 results for: ide

...too!   Using the Data Hub If you're trying to decide which AAV is right for your experiment, searching.... You can check out our step-by-step submission guide or email [email protected] to learn more. A great..., when published, will meet FAIR data-sharing guidelines. But what if my AAVs didn't work?  We love negative...

...My dad was a chemist and he actually gave me the idea of going into law. He said, “you know, the patent...my role is bringing experience and judgement to a wide variety of business decisions that are not “legal...see how I can help. Q: What do you enjoy doing outside of work? BN: I love to travel and that's been difficult...

...share scientific discoveries and developments, and provide tips and tricks for new and seasoned molecular ...specific area of interest. We also cover topics outside of these five areas and you can find what you need...comments below. We’ll track down the story, get the inside scoop, and keep you up-to-date. Be the first to...

...Studies Hybridoma Bank. Antibodies are the most widely used class of protein-binding reagents. In the ...and expanded. The clonal cells are screened to identify hybridoma lines, each producing a monoclonal antibody...ensuring affordable access to materials, repositories provide standardized quality-control and technical support...

...-one CRISPR/Cas9 vectors expressing up to seven guide RNAs (gRNAs). Sakuma said that the new system could...likely to work in animal embryos based on earlier evidence showing that a CRISPR/Cas9 plasmid could be injected...vector-mediated multiplex genome engineering. The system now provides an efficient targeting strategy for multiplex ...

...citation. If you’re considering requesting an Addgene antibody, the Data Hub can help you decide if an antibody... your application. It can be quite difficult to decide on which antibody to request for your experiment...

...student in the Collins' lab at the time) got the idea to engineer bacteriophage (viruses that infect bacteria...bacteria) for use as antibiotics. To develop this idea, Drs. Koeris and Lu founded the biotech startup ... as antibiotics 8:26 - 10:30 : How Dr. Koeris' decided to develop phage for use in a biotech startup 12...

...visited our labs in Cambridge, MA to learn about the wide variety of science careers that Addgenies represent...travels from 2015, including a great European photo slideshow from our UK team member Benoit Giquel. Bluegene...Regional High School Resources at Addgene  Read more Inside Addgene Blog Posts Get Career Advice from Our Bloggers...

...the DNA sequencing reaction should be repeated. Guidelines to help with DNA sequencing troubleshooting and... might suspect contamination in your sample or consider asking your sequencing facility to apply a special...vendors. The precipitation method has an unfortunate side effect of messing up the reaction around base 70...

...Stanford University). Right there on the floor outside the lecture hall. It was wonderful to see the ad-hoc...acoustic biomolecule-based dyes. This technology is widely applicable as bacterial expression of dyes allows...Microbial World’ showcased complex connections and ideas in various visualized approaches from hand-drawn...

...committee creates a compensation philosophy that helps guide employee compensation and also serves as an objective...think is the most important resource that Addgene provides the scientific community? Access to plasmids which...Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog...

...Pooran surveyed a CRISPR google forum to get a better idea of how many people are having trouble, to determine...from Cornell University, is a bit old school and provides a thorough walkthrough of how you can use PCR ...Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science Talk Developing Lab...

...on the Addgene blog and have created and updated guides on various topics in biology over the past year...strategies for generating GFP fusion proteins. From considering protein size and shape, linker sequences, and...Cas9 and reverse transcriptase and a prime editing guide RNA, it can introduce insertions, deletions, and...

...high-throughput manner, but these systems have not been widely employed in vivo. The CHIME and new X-CHIME systems...deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo gene knockout in immune systems...CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions in Mammalian...

...treatment, it is best to identify a cocktail of 3-12 phages. Once these phages are identified, they must then ... forward to help, the Phage Directory team must decide which labs should receive the clinical sample to...

...may have only 2-3 formal supervisors that will provide guidance and experience. Navigating a fulfilling...about mentoring In this blog series, I plan to provide answers to the top questions scientists ask me ...teaches you to fish, doesn’t fish for you) Helps identify a Mentee’s strengths and make the most of them...

...Protein Collection webpage. These plasmids are divided into the following categories: Over 300 empty ...localizes in the cell, use these markers as your guide. As Michael Davidson told the Tallahassee Democrat...go together, we can fix it. Right now, we have no idea." Now, it will be that much easier for scientists...

...viewing sequencing traces, while the paid version provides more tools for plasmid mapping and design (Figure... deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database...molecular biology protocols Browse our protocol video collection ...

...know that the Fc domain provides structure, determines isotypes, and provides a place for secondary antibodies...yes, it does! While the Fc domain’s functions, besides structure and isotype determination, don’t generally...

...reporters that are detectable by an inexpensive and widely available imaging platform: ultrasound. Challenges...experiments because  ultrasound devices are inexpensive, widely available, and have deep tissue penetration and... imaging. Specifically, arg1 expressing E. coli aided in ultrasound imaging of the mouse gastrointestinal...