We narrowed to 911 results for: ire;

1. Illuminating Epigenetics with A FRET Based Biosensor

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   Blog Post

   ... has recently been hitting the headlines, with sotires like the potential devastation of the palm oil ...

2. Experimenting in an Open Source lab: from CRISPR to Cats

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   Blog Post

   ...probably never use the Illumina GAIIx we recently acquired  for sequencing again - it’s too inefficient and...

3. Viral Vectors 101: Producing Your rAAV

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   Blog Post

   ...however, is a “helper-free” system, in which the required adenoviral helper genes are supplied on two different...

4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   ...hundreds to thousands of different pegRNAs for each desired edit, it simply becomes impossible (or at least...

5. Twenty Years of Addgene Sharing: CRISPR

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   Blog Post

   ... blog posts are also frequently read: Homology Directed Repair has been viewed 219,289 times, while Validating...

6. The Scientific Conference Poster Session: Tips for Success

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   Blog Post

   ...gone up to a poster where the presenter spends the entire time talking to the poster, as opposed to engaging...

7. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   Blog Post

   ...-CRISPRi is stably expressed with no selection required and titratable knockdown allows for the study ...

8. PCR Applications for Copy Number Variation Assays

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   Blog Post

   ...accurately read (Bell, 2018.) Even though it does not require a standard curve or a normal control, it can take...

9. Transferable Skills: Negotiation

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   Blog Post

   ...resolution. Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate...

10. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...alternatives Depending on your sequencing result and desired analysis, BLAST may not always be your optimal ...

11. Great Results Start with Great Standard Curves

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    Blog Post

    ...software, don’t give up! Rather than plotting the entire data set, plot only the linear portion. This allows...

12. Uncovering Ancestral Bias in CRISPR Research

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    Blog Post

    ...racial bias from work being done today, is a nearly entirely separate effort from acknowledging its existence...

13. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...And, many wanted to learn more, even those not directly involved in the project.  Once we gauged interest...

14. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    Blog Post

    ... to help the DNA enter the cells. Some samples require a reduced antibiotic concentration or need to be...

15. Plasmids 101: Protein tags

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    Blog Post

    ...cytometry, but rarely used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification...

16. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    Blog Post

    ...pre-set timers for incubation steps, and save the entire process so you’ll have a record of your work. This...

17. Negotiating Work and Life: How to Find the Joy

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    Blog Post

    ...that charming fixer-upper house you have always admired or volunteer to lead a charitable cause.  While...

18. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    .... The supporting data and community engagement required to do this drove Addgene to develop new systems...

19. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post

    ... addition to the neuronal networks involved in directly controlling muscle activity and coordinating movement...

20. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    Blog Post

    ...the production of a cell surface glycan that’s required for ricin uptake. The ricin screen did fail to...