We narrowed to 921 results for: ire;

1. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   Blog Post

   ...endothelial cells from women who are missing an entire X chromosome (TS iPSCs), the lab observed abnormal...

2. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   ...it would take years to develop the mouse lines required for this purpose. Tang wondered if he could use...

3. Antibodies 101: Conjugation

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   ... 2012). And, you can also conjugate antibodies directly to beads for purification purposes.  Coupling...

4. Making CRISPR Plasmids Using Fragmid

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   ... order. (It’s also why you can’t skip a module entirely, but instead will have to replace it with a spacer...

5. Corralling and Cornering the Goldilocks Antibody

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   ...perform differently over time), compatible with the desired applications (such as immunohistochemistry (IHC...

6. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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   ...al., 2019). Finally, scientists can perform the entire process of antibody selection and maturation in...

7. Experimenting in an Open Source lab: from CRISPR to Cats

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   ...probably never use the Illumina GAIIx we recently acquired  for sequencing again - it’s too inefficient and...

8. Tips for Technical Support Calls

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   ...end of that email you’re writing is probably not directly responsible for the problem. We’re here to help...

9. Illuminating Epigenetics with A FRET Based Biosensor

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   ... has recently been hitting the headlines, with sotires like the potential devastation of the palm oil ...

10. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    ...GFP.   Figure 2: Synthetic promoters can be paired for targeted expression. This same pairing of...

11. Early Career Researcher Toolbox: Finding Relevant Papers

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    Blog Post

    ...This is a great way to have new papers delivered directly to your inbox. Best uses Staying up-to-date on...

12. Viral Vectors 101: Producing Your rAAV

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    ...however, is a “helper-free” system, in which the required adenoviral helper genes are supplied on two different...

13. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    ...hundreds to thousands of different pegRNAs for each desired edit, it simply becomes impossible (or at least...

14. PCR Applications for Copy Number Variation Assays

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    ...accurately read (Bell, 2018.) Even though it does not require a standard curve or a normal control, it can take...

15. Transferable Skills: Negotiation

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    ...resolution. Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate...

16. The Scientific Conference Poster Session: Tips for Success

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    ...gone up to a poster where the presenter spends the entire time talking to the poster, as opposed to engaging...

17. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    ...-CRISPRi is stably expressed with no selection required and titratable knockdown allows for the study ...

18. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    ...pre-set timers for incubation steps, and save the entire process so you’ll have a record of your work. This...

19. Twenty Years of Addgene Sharing: CRISPR

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    ... blog posts are also frequently read: Homology Directed Repair has been viewed 219,289 times, while Validating...

20. Tips for Using BLAST to Verify Plasmids

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    ...alternatives Depending on your sequencing result and desired analysis, BLAST may not always be your optimal ...