We narrowed to 1,020 results for: ren

1. Advice for Choosing a Research Project

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   Blog Post

   ...research project “entails good judgement with the current technology to identify a problem, asking what approach...will I take and what can I learn, learning the difference between good and bad data, and knowing how to...

2. Plasmids 101: Antibiotic Resistance Genes

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   Blog Post

   ...how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the ... on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols Find Your Perfect...

3. History of CRISPR Cas - A tale of survival and evolution

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   Blog Post

   ...moieties carry out these functions using slightly different Cas proteins and PAM sequences. A total of eight...involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata...

4. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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   Blog Post

   ...experimental design and usage of these tools, which aren’t always easy to find in publications, so this is...submit data. Please note that the data hub is currently still in beta version, so we’re always looking...

5. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   Blog Post

   ... containing an anti-GFP nanobody fused with a different functional element (tyrosine sulfation consensus...APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP nanobody...

6. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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   Blog Post

   ...studying animals than plants, and biology teachers’ preference for using animal systems to demonstrate biological... a nominal fee for university professors, are currently available through TAIR with a new ABRC database...

7. Antibodies 101: Epitope Tags

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   Blog Post

   ...Ready to start epitope tagging?   References and Resources References Terpe, K., Overview of tag protein...

8. The Golden Stopwatch Award: Recognizing Facilitators of Research

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   Blog Post

   ...of Medicine  Baylor College of Medicine Boston Children's Hospital  Brandeis University  Broad Institute...Studies The Scripps Research Institute St. Jude Children's Research Hospital  Stony Brook University  University...

9. 10 Great Guest Posts We're Thankful For

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   Blog Post

   ...Dr. Polka made R-body mutants that respond to different pH’s and some that are fused to fluorescent proteins...your target gene, activation and repression of different genes in a single cell, and localization of a ...

10. Plasmids 101: TOPO Cloning

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    Blog Post

    ...topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent...may allow you to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia...

11. Another Pathway into Cells: iTOP

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    Blog Post

    ...more efficient and feasible for many labs.  References Efficient Intracellular Delivery of Native ...into all cells? Schwarze SR, Hruska KA, Dowdy SF. Trends Cell Biol. 2000 Jul;10(7):290-5. PubMed. Is VP22...

12. Hot Plasmids: FRET-Based Biosensors

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    Blog Post

    ...enable observation of the interaction between different molecules like zinc and calcium. Ultimately, Merkx... start using it. For us, it’s like a test.” References Thestrup et al. Optimized ratiometric calcium...

13. Guide to Using Pooled Libraries

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    Guide

    ...pooled libraries are single preparations of many different plasmids. Plasmids within a given library have...the same backbone, but they express or target different genes. Some libraries cover the majority of the... library from the Moffat lab has over 175,000 different gRNA-containing plasmids). Amplifying and Using...

14. Early Career Researcher Toolbox: Free Tools for Science Writing

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    Blog Post

    ...same features, but this chart highlights the differences between these two citation managers. If you want...a favorite writing tool, let us know below! References and Resources You Can Grad School podcast episode...

15. 25 Work From Home Tips for Scientists

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    Blog Post

    ...things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule so you can rely ...Most Micropublications contain one figure. If you aren’t there yet, write introductions to potential publications...

16. "Build Your Plasmid": The Game - Play to Teach

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    Blog Post

    ...the game to each a variety of subjects at many different education levels. If you modify BUILD YOUR PLASMID...us at: [email protected] Marco Straccia is currently an Associate Professor at University of Barcelona...

17. Learning Technical Skills at a Lab Central Workshop

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    Blog Post

    ...multiple samples without cross contamination. We currently use the CosMCPrep system for this, but thought... because it forced workshop participants from different biotech companies to interact and learn more about...

18. Trading Spaces - Visiting Scientist Edition

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    Blog Post

    ...provides something that you can’t obtain at your current institute. A common reason is hands-on training... right for you, start thinking outside of your current research box, send out some feelers, apply to programs...

19. Hot Plasmids - January 2023

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    Blog Post

    ...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP Monoclonal...

20. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    Blog Post

    ...in living animals. To do this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11...plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new protein-protein...