Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 916 results for: tro

Showing: 841 - 860 of 916 results

  3. March for Science

    Type
    Blog Post
    ...Additionally, interest groups are often pandered to when strong or overlooked when small, but data is not going...

  10. Typing CRISPR Systems

    Type
    Blog Post
    ..., well understood, and the first taught when introducing CRISPR as a technology. There are three subtypes...

  11. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and...Guides Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral Vectors Vector...Glossary Gamma-retroviruses are a subtype of retrovirus and belong to the Retroviridae family. Gamma-retrovirus...Lentiviruses and gamma-retroviruses fall under the Retroviridae family. The genome of retroviruses is made of RNA...known as tropism). Figure 2: Gamma-retroviral plasmids. Created with BioRender.com. Gamma-Retroviral Vector...Definition Gamma-retrovirus A retrovirus from the Retroviridae family. Characterized by long incubation...decades. Wildtype gamma-retroviruses have been engineered into gamma-retroviral vectors that can be safely...

  12. CRISPR Library Amplification

    Type
    Protocol
    ... mix. Electroporate cells (one at a time for a total of eight electroporations): Electroporator Conditions...centrifuge BioRad Electroporator (MicroPulser TM , Bio-Rad 1652100) Reagents 200 µL electrocompetent cells (Default... 10 cfu/µg) commercially prepared electrocompetent cells is strongly recommended. 800 ng pooled library...and have 1 mL SOC per electroporation readily available for post-electroporation recovery of cells. Ensure... How do I process my Addgene pooled library? Introduction Please refer to our pooled library material ...according to the actual number. Required Quality Control (QC) Pooled libraries can be challenging and expensive...Stbl4 cells or other ultra-high efficiency electrocompetent cells that are suitable for unstable or recombination-prone...

  13. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...single channel pipette Pipette controller Microcentrifuge Electrophoresis chamber Power supply Rocking ... 1mm thick, 10-well, Invitrogen NP0321BOX 20X MOPS SDS running buffer, Invitrogen NP0001 4X NuPage sample...buffer, Invitrogen NP0007 10X NuPage sample reducing agent (500 mM dithiothreitol, DTT), Invitrogen NP0009...Antibodies Recombinant Antibody Purification Protocol Introduction This protocol describes how to determine the...separated by denaturing polyacrylamide gel electrophoresis alongside serial dilutions of a standard antibody...NP0009 PageRuler Plus Prestained protein ladder, Invitrogen 26619 Pipette tips, 10 µL, VWR 76322-132 Pipette...the bottom of the gel. Place the gel in the electrophoresis chamber and secure it. Note: Check the manufacturer...

  14. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ... Protocols Agarose Gel Electrophoresis Agarose Gel Electrophoresis You may also like... Restriction...DNA from an Agarose Gel DNA Ligation Introduction Gel electrophoresis is the standard lab procedure for ...towards the positive electrode. Always Run to Red. Turn OFF power, disconnect the electrodes from the power ...Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation...pairs) for visualization and purification. Electrophoresis uses an electrical field to move the negatively...through an agarose gel matrix toward a positive electrode. Shorter DNA fragments migrate through the gel...protocol video below to learn how to perform gel electrophoresis. Equipment Casting tray Well combs Voltage ...

  15. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Addgene Plasmid Description pLKO.1 – TRC control Negative control vector containing non-hairpin insert. ...serum-free media Invitrogen: #31985 Dulbecco’s Modified Eagle Medium (DMEM) Invitrogen: #11995 Fetal Bovine...Bovine Serum (FBS) Invitrogen: #16000 Penicillin/Streptomycin Invitrogen: #15140-122 Polypropylene tubes...the TRC for expression of shRNAs. pLKO.1 can be introduced into cells via direct transfection, or can be...subsequent infection of a target cell line. Once introduced, the puromycin resistance marker encoded in pLKO...sites in place of the stuffer. The AgeI site is destroyed in most cases (depending on the target sequence.... pLKO.1 – scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid...

  16. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...Pipette controller Benchtop centrifuge compatible with 50 mL conical tubes NanoDrop spectrophotometer 37 °...Immunocytochemistry Protocol Western Blot Protocol Introduction This protocol describes how to affinity purify...concentration of each fraction on the NanoDrop Spectrophotometer using the A280 IgG setting. Combine all eluates...concentration of the pooled sample on the NanoDrop Spectrophotometer using the A280 IgG setting. If the concentration...Determine antibody concentration on the NanoDrop Spectrophotometer. Dilute antibody to 1 mg/mL with PBS if needed...to check the concentration on the NanoDrop Spectrophotometer to see if the sample has reached the desired...Periodically check the concentration on the NanoDrop Spectrophotometer to see if the sample has reached the desired...

  17. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...of each well at 450 nm on the spectrophotometer. Use the spectrophotometer to generate a standard curve...Antibody Purification Protocol Antibody Guide Introduction This protocol describes how to run an indirect...antibody incubation and plate read Tips and Troubleshooting The optimal concentration of primary antibody...an automated ELISA plate washer. Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single...Multichannel pipette reagent reservoirs Pipette controller Microcentrifuge Microplate shaker ELISA plate... antigen Purified recombinant antibody Isotype control antibody HRP-conjugated isotype-specific secondary...mix. Dilute the primary antibody and an isotype control antibody to the desired concentrations in the antibody...

  18. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    [email protected] with any questions. Intro to the Lab Bench Introductory techniques designed to help you get...Antibodies Protocols for common antibody applications. Intro to the Lab Bench Name Description (Link opens in...Quantification Measure DNA concentration with a spectrophotometer Restriction Digests Cut and analyze plasmid...plasmid DNA with restriction enzymes Agarose Gel Electrophoresis Separate DNA by size on an agarose gel Watch...DNA using DNA ligase Bacterial Transformation Introduce DNA into a bacterial strain Watch the Video! CRISPR...Description Link to Video General Transfection Introduce plasmid DNA to mammalian cells Lentivirus Production...Antibodies Name Description Link to Video Transfection Introduce plasmid DNA to mammalian cells to produce antibodies...

  19. Protocol - Bacterial Transformation

    Type
    Protocol
    ...DNA Ligation Introduction Transformation is the process by which foreign DNA is introduced into a cell....should also add a positive control (many companies include a positive control plasmid with their competent...ligation of inserts to vectors than for an intact control plasmid. Incubate the competent cell/DNA mixture...transform large plasmids, it is a good idea to use electro-competent cells. Instead of relying on the heat-shock...heat-shock to cause the cells to take up the DNA, an electro-magnetic field is applied to the cell/DNA mixture... do this you will need to have access to an electroporator and the appropriate cuvettes. Follow the manufacturer's...

  20. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...and FAQ Do controls When doing ligations you should ALWAYS do a vector alone + ligase control. This will...ligation. Additional controls are encouraged, but may only be required for troubleshooting failed ligations...The following table indicates the various controls: Control Ligase Interpretation Uncut vector - Checks...know has only been thawed once before. Always do controls. See Tips and FAQ below for details. Try different...treated, that the phosphatase treatment worked. This control should, in principle, be free of colonies, but ...
Showing: 841 - 860 of 916 results