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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 922 results for: tro

Showing: 841 - 860 of 922 results

  5. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...expressed genes (Emilsson and Kurland, 1990). Controlling gene expression through gene sequence - Proteins...

  7. March for Science

    Type
    Blog Post
    ...Additionally, interest groups are often pandered to when strong or overlooked when small, but data is not going...

  15. Typing CRISPR Systems

    Type
    Blog Post
    ..., well understood, and the first taught when introducing CRISPR as a technology. There are three subtypes...

  16. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and...Vectors Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral Vectors Vector...Glossary Gamma-retroviruses are a subtype of retrovirus and belong to the Retroviridae family. Gamma-retrovirus...Lentiviruses and gamma-retroviruses fall under the Retroviridae family. The genome of retroviruses is made of RNA...known as tropism). Figure 2: Gamma-retroviral plasmids. Created with BioRender.com. Gamma-Retroviral Vector...Definition Gamma-retrovirus A retrovirus from the Retroviridae family. Characterized by long incubation...decades. Wildtype gamma-retroviruses have been engineered into gamma-retroviral vectors that can be safely...

  17. CRISPR Library Amplification

    Type
    Protocol
    ... mix. Electroporate cells (one at a time for a total of eight electroporations): Electroporator Conditions...centrifuge BioRad Electroporator (MicroPulser TM , Bio-Rad 1652100) Reagents 200 µL electrocompetent cells (Default... 10 cfu/µg) commercially prepared electrocompetent cells is strongly recommended. 800 ng pooled library...and have 1 mL SOC per electroporation readily available for post-electroporation recovery of cells. Ensure... How do I process my Addgene pooled library? Introduction Please refer to our pooled library material ...according to the actual number. Required Quality Control (QC) Pooled libraries can be challenging and expensive...Stbl4 cells or other ultra-high efficiency electrocompetent cells that are suitable for unstable or recombination-prone...

  18. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...single channel pipette Pipette controller Microcentrifuge Electrophoresis chamber Power supply Rocking ... 1mm thick, 10-well, Invitrogen NP0321BOX 20X MOPS SDS running buffer, Invitrogen NP0001 4X NuPage sample...buffer, Invitrogen NP0007 10X NuPage sample reducing agent (500 mM dithiothreitol, DTT), Invitrogen NP0009...Antibodies Recombinant Antibody Purification Protocol Introduction This protocol describes how to determine the...separated by denaturing polyacrylamide gel electrophoresis alongside serial dilutions of a standard antibody...NP0009 PageRuler Plus Prestained protein ladder, Invitrogen 26619 Pipette tips, 10 µL, VWR 76322-132 Pipette...the bottom of the gel. Place the gel in the electrophoresis chamber and secure it. Note: Check the manufacturer...

  19. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ... Protocols Agarose Gel Electrophoresis Agarose Gel Electrophoresis You may also like... Restriction...DNA from an Agarose Gel DNA Ligation Introduction Gel electrophoresis is the standard lab procedure for ...towards the positive electrode. Always Run to Red. Turn OFF power, disconnect the electrodes from the power ...Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation...pairs) for visualization and purification. Electrophoresis uses an electrical field to move the negatively...through an agarose gel matrix toward a positive electrode. Shorter DNA fragments migrate through the gel...protocol video below to learn how to perform gel electrophoresis. Equipment Casting tray Well combs Voltage ...

  20. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Addgene Plasmid Description pLKO.1 – TRC control Negative control vector containing non-hairpin insert. ...serum-free media Invitrogen: #31985 Dulbecco’s Modified Eagle Medium (DMEM) Invitrogen: #11995 Fetal Bovine...Bovine Serum (FBS) Invitrogen: #16000 Penicillin/Streptomycin Invitrogen: #15140-122 Polypropylene tubes...the TRC for expression of shRNAs. pLKO.1 can be introduced into cells via direct transfection, or can be...subsequent infection of a target cell line. Once introduced, the puromycin resistance marker encoded in pLKO...sites in place of the stuffer. The AgeI site is destroyed in most cases (depending on the target sequence.... pLKO.1 – scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid...
Showing: 841 - 860 of 922 results