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We narrowed to 938 results for: Kin

Showing: 431 - 440 of 938 results

  1. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...immunohistochemistry images that are crisp, bright, and lacking in non-specific staining can be a challenge in ... mixing your primary and secondary antibodies, blocking excess secondary with serum from the same species...problem.  Pre-block with Fab fragments (Figure 2C) Speaking of Fab fragments… Another strategy for species-on-species...0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody...

  2. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...just on the disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they...proteins. This is, for the intrepid researcher looking for molecular biology tools, a very useful trait...Created using biorender.com   If you’re new to talking about viruses, here’s a quick tip on terminology...reproduce only in experimentally controlled conditions, making them far safer to work with than virions. The four...

  3. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic factors that ...and we’ve been using "plasmid" ever since! From napkins to notebooks Although discovered in the early 1950s...initiated at a Hawaiian deli in 1972. Using a deli napkin for paper, a small group of scientists including... curate, and distribute them all in the name of making it a little bit easier for scientists to conduct...

  4. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...amplification looks like, but by looking at these kinds of graphs, making sure few/no plasmids have dropped... the key points you’ll need to remember when undertaking library amplification and sequencing. Maintain...

  5. Science Career Options

    Type
    Blog Post
    ...Will the job require public speaking? Will you be leading a group or working as part of team? Will you .... It is all too easy to get tunnel vision when working towards a specific goal, and when you realise that...often be misleading. Key aspects to determine are working hours (9am-5pm Mon-Fri or overtime and weekends...

  6. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...established in yeast, including a widely used Parkinson’s experimental model. In fact, approximately 25%...phaffii is highly efficient at protein secretion, making harvesting recombinant proteins a cinch. This is...it doesn’t secrete its own endogenous proteins, making the recombinant protein its major secretion. Beyond...players in the game (even beyond K. phaffii ) worth looking into whether you’re just dipping your toes into...

  7. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...will define positive selection as the means of picking up positive traits (i.e. the plasmid contains a...toxin), whereas negative selection would be the picking up of a negative trait (i.e. the plasmid contains...Conferring Resistance Cell Types* Mode of Action** Working Concentation*** Blasticidin bsd  HeLa, NIH3T3...transfection when creating stable cell lines. Looking for Mammalian Expression Plasmids? Check Out Addgene's...

  8. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...gate which selects anything to the right of it, marking it as GFP+. Quantification for such graphs could...plot! The dreaded smear - fear not! When you are looking to study the level of protein expression, instead...Figure 2). These smears tend to occur when you are looking at proteins that are expressed regularly in cells...percentage positive or negative in your dot plot, looking at a histogram vs antibody plot would be better...

  9. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ... with translatable skills: working with data, working with people, working with complex processes Arm ...Ginkgo Bioworks, our software development team was working on lab operations software for next-gen sequencing...

  10. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ...spread of COVID-19. That’s why we’re proud to be working with Ginkgo Bioworks to synthesize and share over...analyzed first thing the following day. Nothing like waking up to fresh data! These same plasmids are then ...ensuring our rigorous QC standards are met, we are taking a unique hybrid approach compared to our standard...high throughput platforms, Addgene and Ginkgo are striking a balance by modifying certain processes in both...
Showing: 431 - 440 of 938 results