...ideas. What mentors are available for guidance, intelligent discussion, and/or brainstorming sessions to... your interests. We've outlined some important factors to consider and summarize helpful advice from the...Tips to Achieve Success in Academia". Important factors to consider when choosing a research project After...project along the way. Advice from principal investigators Tom Ellis highlights the importance of keeping...patient, being selective, and talking to peers and mentors will help elucidate which project is right for ...

...cytosine base editors to disrupt gene function by targeting start codons, splice acceptor and donor sites...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles... high G+C content genes, as validated through monitoring the expression of fluorescent proteins and oxytetracycline... a) Cells are transfected with three different vectors and flow cytometry is used to sort populations ...CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...

...CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...101: Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments and tired...While the vast majority of CRISPR plasmids in our repository use Cas9 to cut DNA, Addgene Science Communications...generate knockouts, but how does it work? Guest contributors David Wyatt and Dale Ramsden from UNC Chapel...CRISPR/Cas9 and AAV Adeno-associated viral (AAV) vectors are useful for in vivo gene delivery, but have ...

... or LinkedIn to learn more about their career trajectory. If there’s one thing everyone loves, it’s talking...can manifest via research and data gathering, regulatory efforts, policy implementation, program management...company who decides how they are going pursue regulatory approval for a new product or a well-endowed ...move into science policy. Read broadly about the historical roots of science policy to connect to your why...example proposals and consider applying for their accelerator for some hands-on guidance.   Check out my webinar...careers, there are many ways to enter this career trajectory. One common route of entry is through a science...working in student government, and teaching and mentoring others (see more in Figure 2). Starting early ...

...Addgene depositors have made it easier to track translation in two different ways: by monitoring the first...complexes. Recent work has adapted this technique to monitor local translation and determine if proteins are...fusions to their reporter mRNAs. Using translation inhibitors cycloheximide and puromycin, Halstead et al. ...view for constant tracking. You can then directly monitor multiple rounds of translation from this single... Yan et al. used harringtonine, a translation inhibitor that stalls ribosomes after initiation, to calculate...

...lentiviral preps of select lentiviral vectors from the repository for you. We perform test infections with...easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and the reduced titer associated... your gene of interest with a variety of Cas9 activators available in lentiviral format. Finally, while...lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA libraries that can be ...Page Check Out Our CRISPR Guide Pages Find Viral Vectors ...

...expression of the protein. However, expression of receptors may not need to be as high to achieve an observable...titer may not need to be as high when delivering receptor constructs with AAV. In the case of channelrhodopsins...may not be necessary to concentrate this type of vector to such a high titer when looking for effective... possible, use low-bind or siliconized tubes to store samples as it is more difficult for viral particles... advances in recombinant adeno-associated virus vector production." Kidney international 61.1 (2002): ...Where do they come from and what do they mean? AAV Vector Quality Control: Going the Extra Mile with NGS ...

...Research Campus to give researchers the ability to monitor glutamate release from neurons and other brain ...SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging However, the first two generations... domain in the mammalian expression pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol...iGluSnFR3 variants: pRSET: Bacterial expression vector, use with IPTG induction to express soluble protein...is also available in several ready-to-use viral vectors.    References and Resources Additional Resources... 30377363 Aggarwal, Abhi, et al. . “Glutamate indicators with improved activation kinetics and localiation...

...This post was contributed by Matteo Tardelli, a postdoctoral scientist at Weill Cornell Medicine. Discussing...lab meetings with a focus on discussion and brainstorming Though, we might be forced to work from home...of each talk - time for free interaction and brainstorming. This could be achieved by asking each presenter...problem! You can still creatively collaborate and brainstorm! Image from Joseph Mucira.   Implement new...can be used as a break, but can also be used to brainstorm on data in smaller groups, where for instance...blogger Matteo Tardelli! Matteo Tardelli, PhD is Postdoctoral Scientist at Weill Cornell Medicine in NYC and...

...devices to our own interests with the content we store in them: music, emails, chats, pictures, and apps...culture and include a counter, lab timer, dilution calculator, and a couple of other nice features. This app...sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on ...information in the app is restricted to the NEB inventory only, the restriction enzyme sequence resource...acids memorized, but the structures are another story. AMINO has been very helpful when I’ve had to explain...apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega Material...