We narrowed to 1,073 results for: TOR;

...are expressed and purified from plasmids in our repository....summarize antibody production and storage techniques, and explain common applications. View...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...

...into wide use, there were a variety of ways to monitor cell, organelle, and protein localization. For ...the FP timers, add an entire new dimension to monitoring localization; using FP timers, researchers can...Tsuboi et al.), limiting its use. Nonetheless, its creators noticed a few properties that have now become ...benefit of the common FP timers is that you can monitor age by expressing a single FP-timer protein or ... These separate proteins should be useful for monitoring cellular events occurring at varied time scales...fluorescent protein timers FP timers have been used to: Monitor dynamics of vesicle fusion and release from the...vesicle cargoes to FP timer mk-GO (Tsuboi et al.) Monitor gene expression in the developing pancreas by placing...

...Administration of substances to laboratory animals: routes of administration and factors to consider. PubMed, 50...type of injection to use depends on a number of factors. You have to weigh the pros and cons of various...be injected, including cells, drugs, DNA, viral vectors, and viruses. All researchers must go through ...choice for administering different drugs and viral vectors. Like IV injections, intraperitoneal injections...common use of intradermal injections is studying inflammatory responses, as this method is useful for analyzing...for the delivery of various therapies and viral vectors. This injection sounds very disruptive, but surprisingly... This is most often used for delivery of viral vectors, though drug treatments are also possible. One ...

...bacteria, but have no fear - plasmids from Addgene depositors are making it easier than ever to use CRISPR  ...double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage of this...activation of transcription including transcription factors, phage proteins, and RNAP components (Dong et al... al., 2018). They found a transcription factor called SoxS for CRISPRa. Adding complexity, they found ...been observed with Phagemids (Selle et al., 2020, Citorik et al., 2015, Bikard et al., 2014). When the CRISPR... on the presence of specific target sequences (Citorik et al., 2014). These could be genes, specific polymorphisms...refined by the addition of a uracil DNA glycosylase inhibitor and degradation tag on the fusion protein to limit...

...different Cas12a inhibitors. Some of these anti-Cas12a proteins have broad inhibitory effects on Cas12a...Caixia Gao’s lab has developed a new plant base editor from an evolved tRNA adenosine deaminase that is... is fused to a nicking Cas9. This base editor can be used for A•T to G•C edits in protoplasts or plants... Biology Find plasmids containing these base editors Spatially and temporally regulated AAV-based ...single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one overnight...overnight reaction. STAgR is highly customizable as vector backbones, and gRNA structure, numbers, and promoters...Mobile-CRISPRi plasmids Identification of Cas12a inhibitors Using a bioinformatics and a screening approach...

...zinc finger nucleases to transcription activator-like effector nucleases (TALENs). Our most popular kit... Our first popular "kit," Andrew Fire's set of vectors for C. elegans research was our most requested ...expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're familiar with...requests grow for lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two...now have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene...be popular years after they were added to the repository, including beta-catenin reporter M50 Super 8x... GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year in our collection...

...DNA-binding domains based on transcription-activator-like effectors (TALEs) as anchors for genome engineering... “Transcriptional activators are easy to expand or combine with different effectors. I’d encourage people...The results are light-inducible transcriptional effectors (LITEs) designed to bind specific genes and turn...devised the plasmids now available in Addgene’s repository for use in neurons, both in culture and in living...Zhang showed they could package LITEs in viral vectors and genetically target them to specific cell populations...expression levels reliably increased in vitro by a factor of 10 to 20. A flexible and modular optogenetics...

...NHEJ). Designing an RNA editor The Zhang lab envisioned a two-component RNA editor: a Cas13 enzyme fused...after that, the lab published a paper on an RNA editor that allows C to U edits (RESCUE). We’ll walk through...translational machinery treats like guanine. This RNA editor would permit point mutations in RNA which could...nonfunctional. In their quest to build a robust RNA editor, the lab started with the Cas13 scaffold, testing...PspCas13b. Find the plasmids for the RESCUE RNA editors here! Testing and improving RNA editing from the...is too large to fit into adeno-associated viral vectors, they tested truncated ADARDDs to see if they could...edit multiple RNAs. It’s clear that CRISPR RNA editors will open new doors for scientific research - how...

...molecules can alter the overall 260 nm reading, distorting the final concentration calculation and potentially...(Figure 1B). The A260/230 ratio can be a good indicator of protein contamination, but a number of other...contaminants alter this ratio as well (Koetsier and Cantor, 2019). The 280 nm peak is more specific to proteins...researchers traditionally use the A260/280 ratio as an indicator of protein contamination. However, this ratio ...salts, and detergents (Figure 1C-D; Koetsier and Cantor, 2019). Guanidine salts and detergents have absorbance...at concentrations as low as 10 µM (Koetsier and Cantor, 2019). As such, a sample with a good A260/280 ...the presence of RNA contamination (Koetsier and Cantor, 2019). To confirm RNA in your DNA sample, you ...

...papers are analogous to mammalian viral vectors in that the vectors are replication-deficient. Thus, a very...like for organisms like C. difficile. In 2014, Citorik et al. and Bikard et al. developed sequence-specific...antimicrobial, plasmid-based, CRISPR systems (Find the Citorik Plasmids here). In both cases, they used 1-2 CRISPR...sequences found only in certain bacteria (Figure 1). Citorik et al. targeted beta-lactam and quinolone resistance... for bacterial CRISPR targeting. Phage-derived vectors carry CRISPR RNAs (crRNAs) targeting either genomic...suggest that targeting multiple resistance/virulence factors may further reduce this likelihood. The key problem...hardest-to-kill bacteria. In using phage-derived vectors to carry CRISPR, we’re turning the tables on the...