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We narrowed to 971 results for: lars;

Showing: 431 - 440 of 971 results

  1. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ... the digital music revolution hit. I predict a similar revolution for antibodies. Recombinant antibody...that may not work well in animals because of similarities to host proteins. After the antibody of interest...long-term stability, consistency between batches, and molecular definition of recombinant antibodies are essential...US alone (Bradbury & Plückthun, 2015a). With molecularly defined antibodies that do not change over time..., Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are...

  2. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...friendly, to own the process entirely remains a large undertaking. Priority number one was to ensure that... There are regions of some plasmids that are particularly difficult to sequence and assemble, including...regions that are GC rich. Since NGS assembles a larger sequence from many smaller sequences, regions with...some plasmids will not result in one complete, circular assembly. Most of the time, these plasmids are...Viral preps and pooled libraries In addition to regular plasmid sequencing, many of you will be familiar...

  3. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...reproducibility of experiments. Inbred mice are particularly useful for understanding a specific effect related...that alters or eliminates the expression of a particular gene. Targeting vectors used to create knockout... a way to track successfully engineered cells. Similar to transgenic mice, knockout mice are engineered...off-target effects on regions of the genome with similar homology to the site of interest or create mosaic...recombination of floxed sites in the cell can occur. Similar systems include tetracycline or doxycycline mediated...

  4. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...perform more modern techniques used in genetics or molecular biology because the experiments are either not...templates which they can easily generate off of a circular plasmid via PCR. Typically, to make these constructs...complementary DNA (cDNA), and then use PCR and molecular cloning to insert the cDNA into the desired plasmid...those who are interested in performing their own molecular cloning and investigating genes not in this collection...vector backbone. The empty vector PLL4, which is similar to PR-T4P, is available from Addgene. If you use...

  5. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...the bait at a particular point in time, i.e. when the bait is interacting with a particular protein partner...specifically identify proteins that interact with a particular protein  complex as opposed to an individual ...partner or when the bait is part of a particular protein complex. Not performed in a native cell context...bait proteins stop interacting. This feature is particularly useful for in-depth characterization of dynamic...

  6. Tips for Technical Support Calls

    Type
    Blog Post
    ... are your best bet to figure out how to use a particular reagent.  We also have links to later papers ...look! This stuff works!” These papers can be a particularly good resource if you are considering using a...serious science questions, consider using Google Scholar, which searches scientific articles, patents, and.... Addgene keeps a Document of Gratitude from particularly enthusiastic customers who have enjoyed their...combatitive. Not every question is answerable, particularly when it comes to technical questions. BUT, I...

  7. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...CRISPR variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming...Szostak’s lab continues its work on the origin of cellular life. His lab has long hypothesized that life ...Nikolaus Rajewsky from the Max Delbruck Center for Molecular Medicine presented another unexpected finding ...regulatory RNA field. The Rajewsky lab is studying circular RNAs (who knew…I had barely heard of these). These...abundant in neuronal synapses. shRNA silencing of one circular RNA they have identified resulted in a cell migration...

  8. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...Management skills”. The NPA states that Postdoctoral scholars should have the skills and techniques needed to... how to adapt to different situations and, in particular, to different styles of leadership. As the Chair...Committee would change format depending on the particular style and requirements of the new Board Members... more independently and some others would need regular re-direction and alignment with the overall mission...organization to develop these leadership skills in particular because every year it organized a “Committee ...

  9. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...after receiving her degree she was faced with a similar conundrum – she had the skills, training, and motivation...our ideas got pretty good. We started doing extracurricular research on some of them to see how feasible...in my lab notebook from time to time. I wrote in large letters “Going for it!” We kept our day jobs and...company, and that’s what we want it to do. It’s exhilarating to have that sort of freedom. It can also be...and the ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois ...

  10. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ..., there may be particular poster you are interested in viewing which has drawn a large crowd, making it...don’t fully understand the question, ask for clarification. Having them reframe or elaborate their thought...posters you are interested in viewing. For most larger conferences, poster titles and abstracts are available...permitting, you can go back to the most interesting and popular posters at the end of the session. Get in contact...
Showing: 431 - 440 of 971 results