...characterization of the new variants is available here. Upgrades to iGluSnFR3 To improve upon the SF-iGluSnFR, ...

...next-generation sequencing (NGS). For gRNA libraries, you’ll design primers to create 200-300 bp sequencing products...

...with NEB enzymes, the app is quite helpful and provides a list of directions including which buffers to...

...University of British Columbia, Student Services - includes videos on general resume writing, as well as additional...

...run on an agarose gel. The resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from...

... a Good Mentor for Scientists". This post also includes a useful list of questions for a candidate to ...

...oligo overlap cloning, you can design a set of oligos containing your desired restriction sites and add them...that will pay off for years to come. Design Briefly, we will design overlapping oligos that once annealed...procedure will simply differ in terms of primer design). Let's assume that your favorite vector has a ...digest of existing sites in the original vector. Designing oligos To add NdeI, PacI, AscI and MfeI sites ...between the EcoRI and SalI sites of the vector, we design a top oligo with each of the additional sites in...complementary C of the other oligo), but this would destroy the EcoRI and SalI sites in the final vector. Order...

...This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected...Protocol Antibody Guide Introduction This protocol describes how to run an indirect Enzyme-Linked ImmunoSorbent...antigen to validate an antibody. This protocol will describe how to create a standard curve using known concentrations...antibody and an isotype control antibody to the desired concentrations in the antibody dilution buffer....HRP-conjugated isotype-specific secondary antibody to the desired concentrations in the antibody dilution buffer....Check the plate periodically to determine when the desired color change has occurred. The color will change... to optimize experimental conditions. When the desired color change has occurred, gently remove the plate...

...gel Watch the Video! How to Design a Primer Key considerations when designing primers Watch the Video! Sequence...Intro to the Lab Bench Introductory techniques designed to help you get started in the lab. Basic Molecular...antibody applications. Intro to the Lab Bench Name Description (Link opens in a new window) Link to Video Personal...materials on a balance Basic Molecular Biology Name Description (Link opens in a new window) Link to Video Making...without a commercial kit Plasmid Cloning Name Description (Link opens in a new window) Link to Video Restriction... targeting human and mouse genes. Virus Name Description Link to Video General Transfection Introduce ... your lentivirus preparation Antibodies Name Description Link to Video Transfection Introduce plasmid ...

...about a specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV....

... another way, only cells or organisms with the desired phenotype will survive and be assayed at the end...

...focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with actionable steps...

... that you know how to read flow plots and have designed your first flow panel, you’ll load your samples...

...Bhopal. All illustrations / figures featured were designed by our team member Kallol Mozumdar. Sreepadmanabh...

...different phenotypes. For example, this plasmid encodes a green fluorescent protein gene which makes the...

...2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your gRNA. It’s the former version...

...even if science writer Ed Yong’s book “I Contain Multitudes” has me considering the microbiome more often...

... don’t overdo it (some of my best studies were designed over late night poker games at Gordon Conferences...

...have been a model organism for plant research for decades, and the biochemical pathways involved in central...

... phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally PHO8 ...