We narrowed to 1,002 results for: DES

...H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both...

...and workflows where CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly...

...of 173 known synthetic and natural genes.  In designing and optimizing their algorithm, they found that...

...needed for TOPO cloning: 1. Create Your PCR Product: Design standard primers (no need to add unique restriction...

... own experiences founding and running miLab to describe some key lessons I learned while becoming a health...

...Coordinator’ came up at Abcam. Unlike other job descriptions for marketing positions posted on recruitment...

...beginnings of the field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been...

...download the "Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the...

...how can future regulations impact your work? What design variables need consideration? Enrich your knowledge...

...experimental design. Search Addgene's collection of empty LIC cloning vectors Protocol Step 1: Design Your Primers... Primers Primer design for LIC is often as simple as using the backbone manufacturer's suggested leader...primer is shown here. Note: Use web-based primer design software to ensure a melting temperature between...nucleotide dGTP in the reaction (exclude all other nucleotides from standard polymerase protocol), causing the...manufacturer. It is very important to remove all free nucleotides from your PCR product before proceeding, as they...

...next-generation sequencing (NGS). For gRNA libraries, you’ll design primers to create 200-300 bp sequencing products...

...shipment processes. After reading a publication describing how short term storage on dry ice can shift sample...

...on social media or the phones hiding under their desks than listening to me. When I got home, I saw my ...

...store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources on Addgene.org ...

...on Twitter where each poster presenter had four “slides” to use for their poster, eliminating the need ...

...to note that it’s the depositing institute that decides the terms of distribution for their plasmids. ...

...bacterial proteins that bind serotonin. Unger redesigned the binding pocket of the periplasmic binding...

...producer cells and purification protocols often describe a process that begins with cell lysis to release...

...ability to influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists...

...characterization of the new variants is available here. Upgrades to iGluSnFR3 To improve upon the SF-iGluSnFR, ...