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...subtract the weight of the empty tube from the weight of the tube with the gel fragment. The weight of the ...gel in a labeled microfuge tube. Using a scale, weigh the tube with the gel fragment after zeroing the... the gel percentage to get better separation. A higher percentage agarose gel will help resolve smaller...

...sample Using a Light Microscope Learn about the parts of a light microscope and its use Weighing Reagents Learn...applications, with videos for select protocols in the right-hand column. You can find even more video content... Learn how to weigh laboratory materials on a balance Basic Molecular Biology These protocols are the ...

...controller Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5mL...sample of possible dilutions. You may want to try higher/lower dilutions depending on your downstream applications...transduced with lower dilutions of the virus will have higher levels of expression. Consider expanding populations...population may drop. This is because cells that express high levels of the transgene may have reduced growth ...

... homepage! You can track the number of plasmids right below the search bar and more stats further down...

...a: That will warm up the entire lab (hint: you might need some mini marshmallows…). We’ll choose 4 submissions...

...! Our mascot who has supported us over the past eight years now has a new friend to join them. Blugene...

...starting their careers in the lab and even those who might just need a refresher on some best practices for...

... annual #DeckTheLab contest. The bar is set very high from last year’s impressive entries, but we have...

... to me… Antibodies developed by IPI!   🎶On the eighth day of Antibody, Addgene gave to me…  A recombinant...

...counter Reagents Polyclonal stable cell pool DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5...for one 96-well plate. Allow the cells to grow overnight. Replace the medium on the cells and collect the...conditioned medium, it will be more accurate if a higher volume of homogenized cell solution is transferred... Western blotting to screen for lines with the highest or lowest transgene expression ( Figure 2 ). Sample...

...Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric red fluorescent protein.  Listen ...

...editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene gave to me: the PEDAR strategy...

...PES. Using a PES filter will maximize titer . DMEM high glucose, Corning 10-013-CV DMEM, low glucose (1 ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5...dissolved. Pro-Tip This step is challenging due to the high viscosity of PEG. Stirring under medium heat will...precipitation. Precipitation of the viruses can proceed overnight at 4 °C if needed. Transfer the entire sample ... prep . The clarified supernatant can be kept overnight at 4 °C before proceeding with the purification...

...annual #Deckthelab contest. The bar was set very high from last year’s impressive entries, but our community...

...Goedhart  Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with NEB's DADExperts...

...with you.  Top 5 science comics:   1)   “Piled Higher and Deeper” by Jorge Cham   2)   “Boxplot” by Maki...

...Inoculating an Overnight Liquid Culture . After you have bacterial growth, add 500 μL of the overnight culture...bacteria onto an LB agar plate . Grow your bacteria overnight at the appropriate temperature. Growth conditions...page. The next day you will be able to start an overnight culture for plasmid DNA prep the following day...

...in 8,900 institutions across 111 countries. Just eight days into 2024, we've already shipped 2,078 items...

...journey. And if you have additional resources that might be helpful, please reach out to me by email.  About...

...topic page Read Joachim's Series on Choosing the B(right)est Fluorescent Protein Plasmids 101: Visualizing...