We narrowed to 1,104 results for: Ide;

...results. GeoCas9 RNP editing in mammalian cells Besides being able to take some heat, GeoCas9 can introduce...HEK293 cells with GeoCas9 or SpCas9 RNP’s and a guide targeting a site with an overlapping PAM. In both...on Your CRISPR Background with Addgene’s CRISPR Guide Page Find CRISPR plasmids at Addgene ...

...Publishing Institute (SWPI) is an extremely beneficial guide to becoming a successful writer. When I originally...course The online course had three aspects to it: videos starring Roberto Kolter, webinars taught by Peggy... in Washington D.C. at the ASM headquarters. I decided to go ahead and apply; thinking I probably would...

...fact, there was so much useful information that I decided to write a few blog posts. This first post will...the box so the pre-paid postage stamp is on the outside of the container, and drop off the box at any location...sustainability leaders. Take initiative to bring ideas to your workplace and you could end up making a ...

...joined Sanofi/Genzyme nearly three years ago I decided it was time to get in shape. After three kids, ... to raising funds and awareness of FOP. Please consider running with us or making a donation via the link... who is a big superhero fan. This year, I have decided to run as The Green Lantern’s friend, St Walker...

...Integrated DNA Technologies (IDT). “It was fairly accidental. I was finishing graduate school, and didn’t ...these days. I do some public speaking and a bit of video work for webinars (get tips for your next science...bring people in.” Are you interested in careers outside academia like science communication? If so, let...

... how microbiology research is improving lives worldwide and the presentation made it clear how important...up with former colleagues! I ran into past ASM President Tim Donohue (see below) on my way back from getting... with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth. As well as being the Director...

...conversations. This creates a level of familiarity and confidentiality to ensure more honest answers. Ask people who...resources to help you choose a good mentor, I have provided a downloadable PDF at the end of this blog post... may hedge on their answers to your questions. Consider reading Toxic Academic Mentors by @drmellivora...

...of inquiry with which I was not yet familiar. The idea that my piecemeal degree might one day foster a ... several projects nearing deadline (an animated video depicting nuclear fission; a podcast with a genetics... appeal to a given audience and how to utilize a wide array of media in doing so—can prove invaluable ...

...methods for data analysis, our team’s goal is to provide scientists with new tools and protocols that they...labs looking to improve their AAV yields. Given the wide range of downstream applications scientists rely...resources of the Addgene blog Read our beginner's guide to viral vectors Find all of our viral vector blog...

...biological tools, and with the right safety considerations, they aren't dangerous for controlled lab usage...viruses later on. These built-in safety features provide extra layers of risk management when working with...short-term? Answering these questions will help guide you to the right method for your experiment.  A ...

....  These days commercially available TOPO kits provide vectors or cloning arms with overhanging 3´ deoxythymidine... 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase....Molecular Biology Techniques Watch Our Protocol Videos ...

...cellular-level events and behavior. It’s a problem that he considers “one of the greatest challenges of neuroscience...particular advantage for observing events taking place inside cells that are on the move, such as T lymphocytes...protein, troponin C (TnC), from muscle cells. They identified a toadfish TnC variant which had a high-affinity...

...brief history of optogenetics? How long has this idea been around? Boyden: Optogenetics is a toolset that... have tried to make green molecules, but then alongside blue they actually have a lot of crosstalk. So...for the first time? Boyden: There are always considerations for each species. This could be a three or ...

...CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to target the genome...applications when repetitive tRNA sequences must be avoided, different tRNAs with optimized 5’ leader sequences...array for multiplex gene editing applications, we provide detailed manual and online design examples of using...

...coinjection into zygotes of Cas9 mRNA and a single-guide RNA targeting the mutant allele (7). An independent...CRISPR/Cas9 has been used to generate mutations in a wide range of organisms, but its potential to efficiently...These papers suggest it might work well enough to consider as a therapeutic.” There are many fundamental ...

...the pMinDisplay backbone is fused to a specific peptide segment (PDGFR TMD) that anchors the sensor to ...the membrane and displays it on the extracellular side. In addition to the original PDGFR TMD, the researchers...ordered directly from Addgene. The Podgorski Lab has provided a brief breakdown of the promoter systems you ...

...that interact with the virus. As a result, we identified 332 human proteins that the virus interacts with...the help of human proteins in their lifecycle, identifying these potential human helpers of SARS-CoV-2 (...You could be the next scientist joining in this worldwide fight against SARS-CoV-2. As we’ve all been affected...

...gene expression in a target cell. Practical considerations If you have high quality AAV, you can address... desired titer and want to dilute it, err on the side of caution when adding buffer back to the solution... for Work with Virus Watch Our AAV Purification Video ...

... 3 hours. It was robust, capable of generating a wide range of insert sizes and also produced high yields...interact and learn more about one another. They also provided both breakfast, lunch, and snacks. At the end ... to constantly update our processes and get new ideas from learning experiences like workshops at Lab ...

...model yeast strain and discuss why you may want to consider a yeast off the beaten path.   Yeast as a model...disease models established in yeast, including a widely used Parkinson’s experimental model. In fact, approximately...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...