We narrowed to 876 results for: NSI;

1. Plasmids 101: Restriction Cloning

   Type

   Blog Post

   ...stains):  Stain Pre or Post Run? Visualization Sensitivity (ng DNA) SYBR Safe Pre and Post Blue or UV ...

2. Building Global Connections with the International Mentorship Program USA-EUROPE

   Type

   Blog Post

   ...possible a significant qualitative and quantitative expansion in the 2015-2016 academic year. This year, the...

3. A History of Genome Engineering in Popular Culture

   Type

   Blog Post

   ... scientific community and  remind us to always consider the ethics and broader implications of our research...

4. DNA Quantification

   Type

   Protocol

   ...such as TE). You will get much more accurate and consistent readings from DNA in a buffered solution. Reference...

5. CRISPR 101: Multiplex Expression of gRNAs

   Type

   Blog Post

   ...be expressed simultaneously. Vectors for both transient expression and Agrobacterium-mediated transformation...

6. Protocol - How to Design Primers

   Type

   Protocol

   ...for broader pairing capabilities. Taking into consideration the information above, primers should generally...

7. Protocol - How to Perform Sequence Analysis

   Type

   Protocol

   ... experiments. There are a number of factors to consider when selecting sequencing primers, including: ...

8. Protocol - How to Perform a Diagnostic Digest

   Type

   Protocol

   ... to test individual clones before use of more expensive forms of plasmid verification, such as DNA sequencing...

9. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

   Type

   Protocol

   ...Watch the Video! How to Design a Primer Key considerations when designing primers Watch the Video! Sequence...

10. Lab Safety for Biosafety Levels One and Two

    Type

    Protocol

    ...anyone else associated with Addgene, can be held responsible for your use of the information contained in ...

11. Protocol - Bacterial Transformation

    Type

    Protocol

    ...The lowest efficiency cells (usually the least expensive) are fine for transforming plasmid DNA for the...

12. Lentivirus Production

    Type

    Protocol

    ...batch of 1 mg/mL PEI and for each cell line. Considerations Before You Start The health of the packaging...

13. Protocol - How to Run an Agarose Gel

    Type

    Protocol

    ...high percentage of glycerol that increases the density of your DNA sample causing it settle to the bottom...

14. Plasmid Cloning by PCR (with Protocols)

    Type

    Protocol

    ...Cloning The basic PCR primers for molecular cloning consist of: Leader Sequence: Extra base pairs on the 5'...

15. Antibody Validation Using the Indirect ELISA Method

    Type

    Protocol

    ...support reproducibility in science. General Considerations The ELISA can be completed in a single day ...

16. Protocol - pLKO.1 – TRC Cloning Vector

    Type

    Protocol

    ...suitable for handling HIV-derivative viruses. For transient knockdown of protein expression, you may transfect...