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We narrowed to 1,010 results for: PLE

Showing: 861 - 880 of 1010 results

  1. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...range of issues related to AAV vectors, many from people trying to answer the exact same question I was:...Sharing data and community concerns AAV vectors are complex biological reagents; many factors, like storage...

  2. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology... kb cannot be delivered using this vector. For example, although researchers know that Usher Syndrome ...

  3. An Introduction to Adenovirus

    Type
    Blog Post
    ...producer cells where there is E1 and E3 gene complementation. In target cells that are infected by the vector...Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org/...

  4. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... own proteins, making the purification process simpler. Due to its high yields, E. coli is often used ... the purity of your antibody prep. If you have multiple contaminating bands or a few strong ones, consider...

  5. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...incompatible? Plasmid incompatibility occurs when multiple plasmids within one cell have the same replicon...Schumacher, 2012). This concept is a little more complex than simply competing for replication control factors...

  6. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...done by a single enzyme; however, eukaryotes have muliple polymerases which are each responsible for a specific...speaking, promoters in bacteria are less diverse and complex, having fewer parts than those in eukaryotic cells...

  7. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...powerful, but they rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can...Howard M. Salis. "Translation rate is controlled by coupled trade-offs between site accessibility, selective...

  8. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...have various disadvantages and limitations – for example, microtubule-destabilizing drugs are impossible... embryo (Aljiboury et al., 2023). It’s a great example of how optogenetics allows you to disrupt and study...

  9. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918...BL21 strain and derivatives are the most common examples of the E. coli B strain. Common E. coli strains...

  11. New Coomassie Protocol Video

    Type
    Blog Post
    ...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

  12. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ... you don't have to take our word for it!  “When people want to learn about plasmids, they don’t go to ...

  13. CRISPR Guide Update

    Type
    Blog Post
    ...BioRender.com. Figure 1: PRIME editing Please check out the updated guide for a great introduction...

  17. We’re Going Viral This Week!

    Type
    Blog Post
    ... first paper came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to ...

  18. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...Ahem, ahem, drum roll please! It's time to announce the winners of our 2021 Deck the Lab Contest! Though...

  19. The Twelve Days of CRISPR

    Type
    Blog Post
    ...the twelfth day of CRISPR, Addgene gave to me: multiplexed gRNAs.  We hope you’ve been enjoying our CRISPR...
Showing: 861 - 880 of 1010 results