We narrowed to 921 results for: Por

1. Adeno Associated Virus (AAV) for Cell and Gene Therapy

   Type

   Blog Post

   ...basic research and other ongoing clinical trials support the utility of AAV as a broadly applicable gene...

2. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   ... but instead selectively sequence the variable portion of the plasmid in the pool - ex. the gRNA in a ...

3. Plasmids 101: Modular Cloning

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   Blog Post

   ...phenotype, translational output, etc., which can support a larger cellular engineering goal, several of ...

4. Experimenting in an Open Source lab: from CRISPR to Cats

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   Blog Post

   ... funded by someone else (KittyBiome has been incorporated as a non-profit entity and so far most of their...

5. AAV Vector Quality Control: Going the Extra Mile with NGS

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   Blog Post

   ...production process? These questions are especially important when producing multiple vectors in parallel because...

6. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   ...community, will soon expand to include several other important pathways. We invite feedback and contributions...

7. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   ...Researchers from a variety of institutions recently reported their inability to recapitulate the results of...

8. Plasmids 101: Optimizing Plasmid Yields

   Type

   Blog Post

   ... in copy number, the number of plasmid copies supported within each bacterial cell in your culture. Copy...

9. Common Injection Routes in Mice

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   Blog Post

   ...muscle groups (usually the thigh), though it is important to avoid injection into the sciatic nerve. Mice...

10. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    ...different titers are measured. A TCID50 measurement reports an infectious titer, meaning the concentration ...

11. Antibodies 101: Flow Compensation

    Type

    Blog Post

    ...change your compensation values during the analysis portion of your experiment. So, as long as you are able...

12. Of Myc and Men

    Type

    Blog Post

    ... can find it in likely mean it will remain an important tool for the foreseeable future. These newer findings...

13. 10 Basic tips for mammalian cell culture

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    Blog Post

    ...passage are too young and should not be used for important transfection experiments right after they’ve been...

14. Plasmids 101: Protein Expression

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    Blog Post

    ...expression. Despite their ease of use, it is important to note that bacteria usually cannot produce functional...

15. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post

    ...improvement in off-target:on-target editing ratio. Importantly, the observed off-target loci displayed a broad...

16. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post

    ... mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled...

17. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    Blog Post

    ... Signaling and Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody...

18. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    ...virus from the cell pellet alone. If yield is important, or if the viral particles of the AAV serotype...

19. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    ... lab has created a novel nanocompartment for transporting proteins in yeast by using murine polyomavirus...

20. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    ...addition, the use of viruses allows much better spatiotemporal control over labeling methods as compared to...