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  1. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...projecting to the inferior colliculus (IC) are differentially transduced when using the retrograde serotype...significantly updated in 2023 by Jason Nasse.  References 1. Tervo, D. Gowanlock R., et al. "A designer...

  2. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...deliver cargo, it’s important to understand their differences to choose the best one for your experiment. ...Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration of...

  3. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...contain 3 methylases that recognize and methylate different stretches of DNA: Dam methylase adds a methyl...could overlap with a methylation site. The quick-reference table below lists 10 common enzymes that may be...

  4. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...your working directory (the folder that you are currently working in) every time you use them, leave them...guest blogger Kate Palozola! Kate Palozola is currently a graduate student at University of Pennsylvania...

  5. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact, SnapGene...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ...

  6. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...and Gammaproteobacteria species isolated from different bee species. BTK can be used to introduce fluorescent...distributed cell division counting plasmids here References Leonard, Sean P., et al. "Genetic engineering...

  8. Visualizing Protein Turnover In Situ

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    Blog Post
    ...whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization...

  9. Addgene: The First Twenty Years

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    Blog Post
    ...organizations, knocking on doors, and attending conferences. And when CRISPR arrived in 2012, our repository...’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed in response to the...

  10. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post
    ...probably aware that there are several methodologies currently available for approaching it. These include the...even to submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant...

  11. Developing Lab Management Software for Biology

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    Blog Post
    ...fail you. The benefits of lab management software aren’t just limited to large volume facilities; it can...above, any one of these steps can fail but, in our current setup, there are no explicit instructions on what...

  13. Plasmids 101: Cre-lox

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    Blog Post
    ...same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination...

  17. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...dividing up the essential components of a virus into different plasmids. For a retroviral system this looks like... health and safety department if they are not currently provided. Eye protection or face shields are ...

  18. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ... discrimination is especially important in the current outbreak because of the neurological symptoms associated...applications in health, energy, and sustainability.   References 1. Pardee, Keith, et al. "Rapid, Low-Cost Detection...

  20. Hot Plasmids and Viral Preps - May 2021

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    Blog Post
    ...immunoprecipitate the entire complex in three different cell lines. The Gräslund lab plans to continue...of indels to measure time. These plasmids with different promoters driving expression of Cas9 were used...
Showing: 861 - 880 of 1011 results