We narrowed to 1,006 results for: RING;
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TypeBlog Post...edition eBook include: new chapters on genome engineering, cloning, and plasmid tags, addtional content...
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Another Pathway into Cells: iTOP
TypeBlog Post... Mary Gearing... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...Expanding the targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post... track the spatiotemporal dynamics of apoptosis during embryo development. GFP fluorescence increased ... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...stability and minimize misfolding and recombination during virus production needed to deliver CRISPR-Sirius... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...biology, cell biology, microbiology, genetic engineering and strategic knowledge to solve a clinical disease... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...all these reasons, the QCRG is delighted to be partnering with Addgene, who will be taking over the distribution... -
Pushing the Limits of DNA Assembly
TypeBlog Post...sgRNA for CRISPR experiments can be laborious, requiring multiple cloning steps. You can simplify this ... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post...differentiation. Fig. 2: Single cell RNA-seq clustering of cells transduced with the TF pooled library... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post... most often completed your habit is useful for figuring out when to schedule times to write. Pomodoro... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article contributed by... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...thanks to Daniela Bourges for editing this post and sharing her technical expertise in persistent identifiers... -
Science Career Options
TypeBlog Post...When preparing to graduate from university, many students are confronted with the question ‘what now?... -
5 Great Apps for Lab Life
TypeBlog Post...research in terms of preparedness, organization, and sharing data. Here are five FREE science lab apps that ... -
Components of CRISPR/Cas9
TypeBlog Post... endonuclease function, when used for genome engineering, the only CRISPR protein required is the Cas9... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...here's a screenshot from the Chroma Spectra Viewer comparing a standard Cy3 or Rhodamine filter set (Chroma... -
Antibody Guide
TypeGuide...light orange, that bind to a specific antigen, triggering an immune response. The stem of the Y structure...valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary immune response; found mostly in blood...protein:protein complex. Due to their increased manufacturing requirements, monoclonal antibodies are typically...tolerate freeze-thaw cycles. Here are a few tips for storing your antibody: Antibodies conjugated to fluorophores...a similar assay type) and search again. After filtering, narrow down further by company, price, and other...assay with the results of a non-antibody assay measuring either the same output or a proxy for that output... antibody validation This method validates by comparing your antibody of interest to a second, independent... -
Communicating Your Science With Help From ComSciCon
TypeBlog Post...audience your primary focus When writing a paper or preparing a presentation, it's easy to forget that you have... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog Post... Addgene, we now use next-generation sequencing during our QC process. This way, we can confirm the sequence... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...reproducibility. The Trimmer Lab found that in addition to recovering RNA from hybridomas grown in tissue culture, ...