We narrowed to 997 results for: Ran;

1. Supporting Rare Disease Research with BeHeard 2017

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   Blog Post

   ...disease. This year, Addgene will be providing plasmid grants worth $2,000 each to two labs through the BeHEARD...

2. New Norepinephrine Indicators: nLightG and nLightR

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   Blog Post

   ...generating variants with improved affinity and dynamic range of both nLightG and nLightR, and may soon have a...

3. A Better Way to Get Customer Support: The Help Center

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   Blog Post

   ... the entire FAQ collection. You’ll find topics ranging from depositing your lab’s materials and requesting...

4. Future of Research Conference - Remarkable Opening Session

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   Blog Post

   ...practices of indirect costs.  The peer review and grant awards systems need to be radically overhauled.  ...

5. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

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   Blog Post

   ...this type of experimentation to the eventual translation to the clinic and cures. There is no doubt that...

6. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

   Type

   Blog Post

   ...It is clear that one needs to capture a diverse range of CTCs to cope with the variation in cancer, not...

7. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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   Blog Post

   ...target cells that have previously been stably transfected for Cas9 expression. The Yusa lab has used their...

8. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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   Blog Post

   ...expressing ECN is 109 cells/ml, which is within the range of  the ~1011 commensal bacteria per gram of stool...

9. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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   Blog Post

   ...be located. By easily using BLAST to check the translated amino acid sequence from within Snapgene, we ...

10. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

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    Blog Post

    ...preprints, data, software, micropublications) in grant proposals. ReFigures are one such research product...

11. Oh, The Places You Can Go: Careers in Science Communication - Product Development

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    Blog Post

    ...measure, here’s a few other posts about developing transferable skills while in grad school Resources on Addgene.org...

12. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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    Blog Post

    ...New icons can be requested, although there’s no guarantee Biorender will create it. Additionally, you can...

13. We're Updating Our Hold for Publication Policy

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    Blog Post

    ...materials are both excellent ways to provide transparency, support reproducibility, and accelerate science...

14. Antibodies 101: Affinity Tags

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    Blog Post

    ...blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with...

15. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

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    Blog Post

    ...matter expert in the educational sphere, it can range from researching an area, to writing an introduction...

16. Bricking Science: Portraying Scientific Reality Through LEGO

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    Blog Post

    ...have the same issues as one doing experiments in France. We can learn with each other through globalized...

17. Tips for Improving Your Next Manuscript

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    Blog Post

    ...worthless. But simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts...

18. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    Blog Post

    ...motto for scientists who contact us and want a guarantee that their experiments will work (yes, this happens...

19. Choosing a Good Mentor for Scientists

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    Blog Post

    ... opportunities to learn and practice successful grant writing, lab budgeting, equipment acquisition, public...

20. Virus Protocol - Generating Stable Cell Lines

    Type

    Protocol

    ...Different brands and FBS lots can promote or inhibit transfection. Test a variety of brands and FBS lot...-term protein expression observed using transient transfection approaches, generating cell lines using...experiments in a stable cell line, as opposed to transiently-transfected cells, increases reproducibility, as it... the variation associated with repeated transient transfection. Some lentiviral vectors deliver mammalian...stable cell culture after transduction. Performing antibiotic selection on transduced cells enables elimination...Pro-Tip Transducing too many cells relative to the number of virus particles reduces the transduction efficiency... may have 1 copy of the transgene, while others have >1 copy of the transgene in their genome. This can...