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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,044 results for: STI;

Showing: 861 - 880 of 1044 results

  1. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...to identify cells that took up the vector and distinguish between plasmids that contain the gRNA (white...Walkowski.   Restriction digest Another way to distinguish between colonies containing plasmids with inserts...

  2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ..., integrated reporter for assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA...integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Journal of Biological...

  4. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ... from their native conditions of ~0°C, which drastically affects their stable folding and efficient functioning...beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric science, our team has continuously...

  6. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...promoters, are always active and thus referred to as constitutive promoters. Others are only active under specific...complexed with cAMP activator protein (CAP) can also stimulate AraC binding to I1 and I2 sites. Supplementing...

  8. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... a jigsaw puzzle, the oligos were designed with sticky ends that only anneal together in one direction...plasmids available from Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them...

  10. Hot Plasmids - February 2022

    Type
    Blog Post
    ...the Genetic Pertubation Platform at the Broad Institute have expanded the genetic screening toolkit by...separately. The proteins, which are purified via histidine tags, are optimized for SDS-PAGE gels and can ...

  11. Antibodies 101: Isotypes

    Type
    Blog Post
    ...isotype? An isotype determines several key characteristics of an antibody as well as the role it plays... regions result in several unique physical characteristics of these five isotypes, such as the number ...

  13. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...gold standard in gene expression analysis to investigate the effect of regulatory elements — such as promoters...interaction occurs, the luciferase emits its characteristic light. However, if a protein interaction brings...

  14. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...making virus’ or what gag and pol stand for, we demystify all components of viral vectors here! Need Virus...into the host genome (more on this below), it’s still used to deliver gene expression constructs. Sandwiched...

  15. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...producing the proteins – are not, and many of the existing GCE tools are not very robust. When using GCE,...work. Plus, keep an eye on our blog – we’ll be posting more posts on the various protocols and helping...

  16. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...prototypical gamma-retrovirus.    Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses...post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes via increased nuclear...

  17. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...application. These controls will allow you to distinguish real results from background noise or nonspecific...positive and negative cells. This can be achieved by adjusting your voltage using your single color controls ...

  18. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...Image courtesy of National Human Genome Research Institute. Its genome, however, isn’t enough to ensure...their low immunogenicity, which means they do not stimulate a strong immune response when introduced into ...

  20. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...less than an hour, thus a population of cells constitutively expressing a targeted Cas9 should possess indels...reduces breaks and mutations at off-target sites. Christina Mork contributed to the update of this post. Thank...
Showing: 861 - 880 of 1044 results