We narrowed to 1,076 results for: TOR
-
TypeBlog Post...limitations of packaging size associated with viral vectors. For these reasons, iTOP, especially in tandem ...
-
Handling Plasmids from Addgene - Purifying Plasmid DNA
TypeProtocol... mM EDTA Store Solution I at 4°C Solution II - Denaturing Solution 0.2 N NaOH 1.0% SDS Store Solution ...acetate 23 mL glacial acetic acid 57 mL of dH 2 O Store Solution III at 4°C Grow 2 mL overnight cultures... room temperature before quantifying and using. Store DNA at 4°C. Tips and FAQ Plasmid purification kits... -
25 Work From Home Tips for Scientists
TypeBlog Post...radio reporter challenge, and code.org has coding tutorials for all ages. Organize your data and prepare ... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post...to small fluorescence changes of the donor and acceptor after proteolytic cleavage. Secondly, fluorescence... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...turn genes on or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in ... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...poring over my grandparents’ medical textbooks by torchlight under the covers. In high school, I went to all... -
How to Lead a Great Meeting
TypeBlog Post... Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a Research ... -
Components of CRISPR/Cas9
TypeBlog Post... to modify the desired genomic locus. Some historical notes CRISPR arrays in bacterial genomes consist... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post...Purification Protocol Video rAbs in the Addgene repository... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post... any other required non-CRISPR-associated host factors required for CRISPR processing. For each enzyme... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...these strains should not be used for long term storage. Additional Resources Review of restriction modification... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog Post...You never know when you will meet a potential collaborator Some of these people you meet will review your... -
Transferable Skills Guide: Problem Solving
TypeBlog Post... you can start by paying attention to how your mentors reach solutions and ask them to explain their logic... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https... -
Antibodies 101: Fab Fragments
TypeBlog Post...secondary antibodies you already have in your refrigerator will not recognize a Fab fragment. In order ... -
To Each HIS Own
TypeBlog Post...affinity purification of human insulin-like growth factor I from culture medium of E.coli. Bio/Technology... -
Plasmids 101: Cre-lox
TypeBlog Post...independently of any other accessory proteins or co-factors, thus allowing for broad applications in a variety...