We narrowed to 956 results for: cha
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TypeBlog Post...secondary antibodies to bind. We also know that changing the Fc domain can impact an antibody's binding...
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Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post... the type of phenotype to look for with an uncharacterized gene. The actual reporter construct can be... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post...was described. Using Cellosaurus Cellosaurus is searchable by cell line name (recommended names and synonyms... -
Antibodies 101: Affinity Tags
TypeBlog Post...amylose resin and also provides a visual detection mechanism via applications such as western blot and immunofluorescence... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post...sequences and even entire genes. Figure 1: Mechanism of CAST systems. A Cas effector (Cascade in Type... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog Post... ReFigures in a few minutes, visit our YouTube Channel. Currently we can only connect figures from PubMed... -
Tips for Improving Your Next Manuscript
TypeBlog Post...two of our colleagues’ manuscripts and discuss changes to particular sections. The focus groups allowed... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post...people dress up like superheroes or other iconic characters and run a 5K around Cambridge. We will be collecting... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post...continents who dropped by the booth. We were happy to chat with lots of researchers who weren’t aware of Addgene... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post...for about $10 US dollars worth of glass powder pruchased from a pottery supply house. These large, cheap... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post... antibiotics commonly found in the lab, their mechanism for killing bacteria, and general working concentrations... -
Choosing a Good Mentor for Scientists
TypeBlog Post...conflicts are a common reason for scientists to change labs – do as much as you can to ensure a good fit... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post...derivatives that generate covalent bonds with positively-charged amino acids. Unfortunately, the location of biotin... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog Post...developmental programs unfold, absorbing the stochasticity of molecular interactions and embedding individual... -
Plasmids 101: Origin of Replication
TypeBlog Post... so please, post in the comments. References Chatterjee A, Sun SB, Furman JL, Xiao H, Schultz PG. A versatile... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...sequencing conditions which can read through this challenging sequence. Transgene The transgene is the gene... -
Meet the New Addgene Board Members!
TypeBlog Post...Therapy. Vandenberge is also the Grousbeck Family Chair in Gene Therapy, Director of the Grousbeck Gene ... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog Post...material transfer agreement Technology Transfer: the exchange of intellectual property (as inventions, ideas... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...TM, Clayton KL, Nitido AD, Murali MR, Alter G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D,... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...activate transcription by causing conformation changes in the AraC dimer. In doing so, the dimer shifts...