We narrowed to 1,046 results for: fer
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TypeBlog Post...of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene... of how miRNAs regulate their targets and what confers specificity in the miRNA regulatory process. This...
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Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post... proteins whose excitation and emission spectra differ sufficiently for them to be imaged in distinct ...fluorescent channels on the microscope. With the proliferation of fluorescent proteins in recent years, there... -
Viral Vectors 101: Types of viruses
TypeBlog Post...high and your immunogenicity low! References and Resources References Robinson HL. Retroviruses and cancer... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...SARS-CoV-2 proteins for downstream experiments and offer flexibility in tag, cleavage, codon usage, and expression...Visit our COVID-19 Plasmids and Resources Page! References Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...culture). This dilution step represents the major difference between PACE and PANCE: for PANCE the phage progeny...much more efficiently and in more contexts. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...the system and the flexibility to address many different types of research questions. Are you ready to ...experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera. "The continuously... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...Listen to the C. elegans RNAi podcast segment RNA interference (RNAi) is extensively used in C. elegans research...endogenous RNAi pathways. These mutations however can interfere with other genetic pathways making it difficult... -
Hot Plasmids - May 2022
TypeBlog Post...peptide. By combining three different localization peptides with four different fluorescent proteins, and... -
Antibodies 101: ChIP
TypeBlog Post... the cutting. While sonication is usually the preferred method, enzymatic digestion can be appropriate...protocols when embarking on your ChIP journey! References and Resources More resources on Addgene’s blog... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post... web of opportunities that the classroom cannot offer. Furthermore, companies across most industries tend...helps you find internships and scholarships in different subject areas! 2. Resume and cover letter... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post...method’s flexibility allows it to be used with different types of PCR-generated inserts. By harnessing ...updated by Emily P. Bentley in September 2024. References and Resources Further reading Harnessing homologous... -
Plasmids 101: Inducible Promoters
TypeBlog Post...and SV40 promoters, are always active and thus referred to as constitutive promoters. Others are only ...Experimental System Because transcription machinery differs between cell types or organisms, promoters are ... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post... and precisely identifies plasmid features that differ by only a few nucleotides, such as the fluorescent...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ... -
The time and cost required to make a plasmid
TypeBlog Post...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...period. For ease of calculations*, let’s split the difference and put scientific inflation at… roughly 34.9%... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog Post... invasive to use optogenetics in the brain. References Bedbrook CN, Yang KK, Robinson JE, Mackey ED,...Read more about optogenetics Learn about the differences between chemogenetics and optogenetics Resources... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...al. applied TRIM-away to nine proteins in ten ddifferent cell types, including difficult-to-manipulate...that TRIM21 overexpression did not affect cell proliferation. These cells also did not contain changes in... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...function of the protein and pdDronpa are preserved. References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, ...28232577. Figures 2-4 were modified from this reference. 2. Regot, Sergi, Jacob J. Hughey, Bryce T. Bajar... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...us develop these resources further. Feel free to offer your comments and suggestions, and even to submit...submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant DNA ... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post... plasmid libraries containing combinations of different gRNAs in each plasmid. Each gRNA combination can...systems. He can be contacted at [email protected]. References 1. Cheng, Allen A., Huiming Ding, and Timothy... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...virtual bookshelf. Pro tip The “cited by” and references sections of a PubMed entry are great places to...This library can also be synced with a Mendeley reference manager account. Pro tip Sign up for weekly email...