We narrowed to 1,107 results for: ide

...Integrated DNA Technologies (IDT). “It was fairly accidental. I was finishing graduate school, and didn’t ...these days. I do some public speaking and a bit of video work for webinars (get tips for your next science...bring people in.” Are you interested in careers outside academia like science communication? If so, let...

...results. GeoCas9 RNP editing in mammalian cells Besides being able to take some heat, GeoCas9 can introduce...HEK293 cells with GeoCas9 or SpCas9 RNP’s and a guide targeting a site with an overlapping PAM. In both...on Your CRISPR Background with Addgene’s CRISPR Guide Page Find CRISPR plasmids at Addgene ...

...fact, there was so much useful information that I decided to write a few blog posts. This first post will...the box so the pre-paid postage stamp is on the outside of the container, and drop off the box at any location...sustainability leaders. Take initiative to bring ideas to your workplace and you could end up making a ...

...viewing sequencing traces, while the paid version provides more tools for plasmid mapping and design (Figure... deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database...molecular biology protocols Browse our protocol video collection ...

...know that the Fc domain provides structure, determines isotypes, and provides a place for secondary antibodies...yes, it does! While the Fc domain’s functions, besides structure and isotype determination, don’t generally...

... generosity makes it possible for researchers worldwide to access the tools they need, accelerating scientific...educational resources, you're an essential part of a worldwide community helping scientists access the tools ...part of ours. We’re grateful to move into 2026 alongside you, strengthening the foundations of science ...

...Publishing Institute (SWPI) is an extremely beneficial guide to becoming a successful writer. When I originally...course The online course had three aspects to it: videos starring Roberto Kolter, webinars taught by Peggy... in Washington D.C. at the ASM headquarters. I decided to go ahead and apply; thinking I probably would...

...joined Sanofi/Genzyme nearly three years ago I decided it was time to get in shape. After three kids, ... to raising funds and awareness of FOP. Please consider running with us or making a donation via the link... who is a big superhero fan. This year, I have decided to run as The Green Lantern’s friend, St Walker...

... how microbiology research is improving lives worldwide and the presentation made it clear how important...up with former colleagues! I ran into past ASM President Tim Donohue (see below) on my way back from getting... with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth. As well as being the Director...

...conversations. This creates a level of familiarity and confidentiality to ensure more honest answers. Ask people who...resources to help you choose a good mentor, I have provided a downloadable PDF at the end of this blog post... may hedge on their answers to your questions. Consider reading Toxic Academic Mentors by @drmellivora...

...biological tools, and with the right safety considerations, they aren't dangerous for controlled lab usage...viruses later on. These built-in safety features provide extra layers of risk management when working with...short-term? Answering these questions will help guide you to the right method for your experiment.  A ...

...of inquiry with which I was not yet familiar. The idea that my piecemeal degree might one day foster a ... several projects nearing deadline (an animated video depicting nuclear fission; a podcast with a genetics... appeal to a given audience and how to utilize a wide array of media in doing so—can prove invaluable ...

...methods for data analysis, our team’s goal is to provide scientists with new tools and protocols that they...labs looking to improve their AAV yields. Given the wide range of downstream applications scientists rely...resources of the Addgene blog Read our beginner's guide to viral vectors Find all of our viral vector blog...

....  These days commercially available TOPO kits provide vectors or cloning arms with overhanging 3´ deoxythymidine... 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase....Molecular Biology Techniques Watch Our Protocol Videos ...

...cellular-level events and behavior. It’s a problem that he considers “one of the greatest challenges of neuroscience...particular advantage for observing events taking place inside cells that are on the move, such as T lymphocytes...protein, troponin C (TnC), from muscle cells. They identified a toadfish TnC variant which had a high-affinity...

...brief history of optogenetics? How long has this idea been around? Boyden: Optogenetics is a toolset that... have tried to make green molecules, but then alongside blue they actually have a lot of crosstalk. So...for the first time? Boyden: There are always considerations for each species. This could be a three or ...

...gene expression in a target cell. Practical considerations If you have high quality AAV, you can address... desired titer and want to dilute it, err on the side of caution when adding buffer back to the solution... for Work with Virus Watch Our AAV Purification Video ...

... 3 hours. It was robust, capable of generating a wide range of insert sizes and also produced high yields...interact and learn more about one another. They also provided both breakfast, lunch, and snacks. At the end ... to constantly update our processes and get new ideas from learning experiences like workshops at Lab ...

... Science. For each publication, Web of Science provides basic bibliographic information and uses its vast...is very plausible that science published in more widely-read journals gets more citations and is more likely...citations due to the journal of publication. Table 1 provides an example calculation of the weights. Table 1...

...center around a cast of endearing characters who decide to take matters into their own hands. Who doesn..., I wanted to be challenged to read some books outside of my comfort zone. I’d been in a bit of a reading.... We’ve read well-known classics, like Austen’s Pride and Prejudice, and some more obscure treasures, ...