We narrowed to 931 results for: por

1. New Tool for Lineage Tracing: The ClonTracer Library

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   ...leukemia. Bhang says the work highlighted the importance of developing more potent ABL1 allosteric inhibitors...

2. Plasmids 101: Golden Gate Cloning

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   ...like Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs...

3. Plasmids 101: Cre-lox

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   ... Plasmids 101 series, we examined a number of important plasmid elements – promoters, origins of replication...

4. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   ...while also capturing information about spatial and temporal organization. The design use to detect PKA activity...

5. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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   ...basic research and other ongoing clinical trials support the utility of AAV as a broadly applicable gene...

6. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   ... but instead selectively sequence the variable portion of the plasmid in the pool - ex. the gRNA in a ...

7. Adenoviral Vector Production and Troubleshooting

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   ... quickly due to immune response against AdV. Importantly, recombination-competent adenovirus (RCA) can...

8. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   ...Hybridization (WISH) WISH is a technique that detects the temporal and spatial localization of mRNA transcripts in...

9. Plasmids 101: The Wide World of Natural Plasmids

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   ...plasmids — more on that in a minute! Fun fact! Electroporation is one popular method scientists use to promote...

10. Plasmids 101: Modular Cloning

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    ...phenotype, translational output, etc., which can support a larger cellular engineering goal, several of ...

11. Tips for Titering Your Lentiviral Preps

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    ...the sample regardless of whether or not it is incorporated into a viral particle. Consequently, titers ...

12. Experimenting in an Open Source lab: from CRISPR to Cats

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    ... funded by someone else (KittyBiome has been incorporated as a non-profit entity and so far most of their...

13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...improvement in off-target:on-target editing ratio. Importantly, the observed off-target loci displayed a broad...

14. Hot Plasmids and Viral Preps - May 2021

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    ... lab has created a novel nanocompartment for transporting proteins in yeast by using murine polyomavirus...

15. Tips for a 1st time AAV user (by a Rookie AAV user)

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    ...virus from the cell pellet alone. If yield is important, or if the viral particles of the AAV serotype...

16. 10 Basic tips for mammalian cell culture

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    ...passage are too young and should not be used for important transfection experiments right after they’ve been...

17. Plasmids 101: Protein Expression

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    ...expression. Despite their ease of use, it is important to note that bacteria usually cannot produce functional...

18. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ... Signaling and Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody...

19. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ... mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled...

20. AAV Vector Quality Control: Going the Extra Mile with NGS

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    ...production process? These questions are especially important when producing multiple vectors in parallel because...