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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 994 results for: Ran

Showing: 881 - 900 of 994 results

  5. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...Different brands and FBS lots can promote or inhibit transfection. Test a variety of brands and FBS lot...-term protein expression observed using transient transfection approaches, generating cell lines using...experiments in a stable cell line, as opposed to transiently-transfected cells, increases reproducibility, as it... the variation associated with repeated transient transfection. Some lentiviral vectors deliver mammalian...stable cell culture after transduction. Performing antibiotic selection on transduced cells enables elimination...Pro-Tip Transducing too many cells relative to the number of virus particles reduces the transduction efficiency... may have 1 copy of the transgene, while others have >1 copy of the transgene in their genome. This can...

  15. Western Blot

    Type
    Protocol
    ...Gel Transfer Device. Wet the pre-run gel in deionized water and place it on the transfer membrane of the...protocol uses a dry transfer device but can be adapted for wet and semi-dry transfer approaches. Last Update...well. Assemble the transfer sandwich as follows: Unseal the iBlot 2 PVDF Mini transfer stack. Set the Top... parameters are correct. Transfer for 5–6 min for proteins <30 kDa. Transfer for 8–10 min for proteins...before running your experiment. Remove the membrane from the transfer device and place it in a container with...Block the membrane in blocking buffer for 1 h at RT on a shaking platform. Wash the membrane 3x for 5 ...Incubate the membrane with secondary antibody for 60 min at RT on a shaking platform. Wash the membrane 3x for...

  16. Lentivirus Production

    Type
    Protocol
    ...Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands and lots...lentiviral vector transfected into 293T cells using a polyethylenimine (PEI) transfection protocol. This ...packaging cells Day 1 (pm): Transfect packaging cells Day 2 (am): 18 h post-transfection. Remove media, replace...filter Transfection grade DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer plasmid... optimized for each transfer plasmid. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...modified for alternative packaging cell lines or transfection reagents. Once produced, lentivirus can be used...drifted. Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into sterile tubes. Store...

  17. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...drives RNA Polymerase III transcription for generation of shRNA transcripts. cPPT Central polypurine tract...HIV-derivative viruses. For transient knockdown of protein expression, you may transfect plasmid DNA directly...Day 2: b. Perform the transfection in the late afternoon because the transfection mix should only be incubated...it, and does not grant any license, express or implied, under any patent. All transfers of materials from...protocol is provided for your convenience. See “warranty information” in appendix. Table of Contents A....Digesting pLKO.1 TRC-Cloning Vector C.4 Ligating and transforming into bacteria D. Screening for Inserts D.1 Recommended...Sequence of pLKO.1 TRC-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning ...
Showing: 881 - 900 of 994 results