We narrowed to 1,056 results for: STI

...each from a constitutively active CMV promoter. Constructs were then verified by both diagnostic restriction...

...the Genetic Pertubation Platform at the Broad Institute have expanded the genetic screening toolkit by...separately. The proteins, which are purified via histidine tags, are optimized for SDS-PAGE gels and can ...

...isotype? An isotype determines several key characteristics of an antibody as well as the role it plays... regions result in several unique physical characteristics of these five isotypes, such as the number ...

...Monoclonal Versus Polyclonal Antibodies: Distinguishing Characteristics, Applications, and Information Resources...

...to identify cells that took up the vector and distinguish between plasmids that contain the gRNA (white...Walkowski.   Restriction digest Another way to distinguish between colonies containing plasmids with inserts...

..., integrated reporter for assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA...integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Journal of Biological...

...certain sites, or make only a subset of base substitutions.  The need for speed: base editing before Cas...compared to the original TadA-7.10 enzyme. After testing numerous TadA variants, they identified TadA-8e...

... from their native conditions of ~0°C, which drastically affects their stable folding and efficient functioning...beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric science, our team has continuously...

...fluorescent to the surface of the bead, suggesting that OptoNBs still bound their target protein even when...

...commercial antibody against them. There’s also a question about how specific those interactions are. This... that, but the system is so flexible. Other investigators can easily adapt these vectors for use with ...

...less than an hour, thus a population of cells constitutively expressing a targeted Cas9 should possess indels...reduces breaks and mutations at off-target sites. Christina Mork contributed to the update of this post. Thank...

.... Staining with multiple HRP-RANbodies is indistinguishable since they produce the same signal, so only...fluorescent protein (i.e. GFP, RFP) Detecting GFP reconstitution across synaptic partners (GRASP) assays No...

...proteins interact or oligomerize in cells? This question is largely unanswered. Ideally, fluorescent proteins...cells (Shaner et al, 2013; Bindels et al, 2017). Still, the data presented in papers should be used with...

...Maybe take a deep breath?" Once you answer the question, you need only to take that step. Repeat that ... to be anxious, nervous, and/or apprehensive. I still get nervous quite regularly, and I've had multiple...

... while puzzling through your broader research questions and designing future experiments. Being able to...Scientific Method? Imagine your problem is just a question you have about the world. You might do some background...

...their activity in mammalian systems has been in question. Three different labs have recently published ... the prime editor, yielding PEmax, to work synergistically with epegRNAs and PE4 and PE5. These enhanced...

...sensory input from our extremities. “Here, painful stimuli are processed by a network of excitatory and inhibitory... demonstrate pain response due to blue light stimulation of dual virus-expressing CR neurons. (C) Duration...

...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...increases the likelihood that one of the clones will still recognize your protein. For the same reason, they...

... in which the hM4Di was fused to mCherry. To investigate the localization of the hM4Di, we used primary...the membrane in mice neurons, most of them were still cytoplasmic. Use of ‘small’ tags to identify proteins...

...Morgan, our senior engineer? The answer to these questions--which were real, not hypothetical--was clearly...Others in the meeting might also have the same question and asking can help the team identify possible...