We narrowed to 958 results for: ARC

1. Giving gRNAs a Facelift - Synthetic and Beyond

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   ...stable than most macromolecules used in scientific research such as DNA or protein. It comes as no surprise...

2. Antibodies 101: Selecting the Right Antibody

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   ...experiments require antibodies. You run a quick internet search for the required antibodies and find….hundreds ...

3. Lighting Up Cell Signaling with Photoswitchable Kinases

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   ...photoswitchable kinases in vivo with C. elegans. Researchers commonly use rectal bacterial infection to induce...

4. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   ...Levine, B. (2010). Methods in Mammalian Autophagy Research. Cell,140(3), 313-326. doi:10.1016/j.cell.2010.01.028...

5. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ...protocols! We invite feedback from all members of the research community to help us develop these resources further...

6. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   ...Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi:10.1093/nar/gku1198...

7. Antibodies 101: Stripping and Reprobing Western Blots

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   ...reprobe your western blot several times — some researchers report up to ten cycles! Just remember that a...

8. Progress Towards a PAM-Free CRISPR

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   ...alleles, and the growing need for gene editing in research, the old PAM constraints just won’t cut it anymore...

9. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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   ...half-lives. Interested in using Split-BioID with your research? Plasmids are available from Addgene! References...

10. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ... viral protocols and videos Find AAVs for your research ...

11. Hot Plasmids and Viral Preps - January 2021

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    ...fluorescence-activated cell sorting (FACS) helps researchers sift through thousands of cells to identify cells...

12. Antibodies 101: Choosing the Right Isotype

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    ...After all, there’s no sense in spending valuable research dollars on a new secondary antibody if you don...

13. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...p300 or the DNA methyltransferase DNMT3A allows researchers to easily add biochemical marks to genetic regions...

14. Fluorescent Proteins 101: Introduction to FRET

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    ... Before constructing your own FRET probe, try searching PubMed for articles describing the FRET tool that...

15. Plasmids 101: Terminators and PolyA signals

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    ...expression in a context-dependent manner. Nucleic Acids Research. 2000. PubMed PMID:10648781. PubMed Central PMCID...

16. Tips for Titering Your Lentiviral Preps

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    ...resistance-genes, or the transgene itself. Many researchers prefer to design primers that target the common...

17. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    ... to Expedite Your Experiments and Achieve Your Research Goals    ...

18. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post

    ...knockdown, however, keeps bacteria viable and allows researchers to study if that gene is a target of an antibiotic...

19. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ...using adenoviral vectors with your CRISPR/Cas9 research! To find more information about the adenoviral...

20. Polymerase Chain Reaction Overview and Applications

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    ... was first identified in the late 1960s during research at hot springs in Yellowstone National Park.  ...