We narrowed to 957 results for: Cre

1. Antibodies 101: Flow Cytometry Controls

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   ...cells but with far more predictability. The beads create an artificial positive (bound) and negative (unbound...

2. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   ...many major cancer genes. These should be used to create ever more complete “look-up-tables” of mutant function...

3. GCE4All: Making Genetic Code Expansion Accessible

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   ...The catch The rapid expansion of GCE led to the creation of many new tools and potential applications. ...

4. AAV Q&A with Tim Miles

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   ...flexibility of AAV genome design in remarkably creative ways. The continued development of more and smaller...

5. Antibodies 101: Buffers, Storage, and Conjugates

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   ...basic guidelines below, you’ll likely be able to create a small, usable panel.  First, fluorophores vary...

6. Antibodies 101: Isotypes

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   ... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

7. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ...signaling pathway.  As a proof-of-principle, the team created an OptoNB to control the Ras/Erk signaling pathway...

8. Plasmids 101: Methylation and Restriction Enzymes

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   ...keep in mind that dam-/dcm- strains may have an increased rate of mutation (as these would also be deficient...

9. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   ...harnesses the power of bacterial recombineering to create insertions, deletions, and substitutions - at the...

10. CRISPR 101: Non-Homologous End Joining

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    ...Genome-Wide Control for Large-Scale Functional Screens        ...

11. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    ...can make a big difference, if you can find one. Create your own peer mentoring circle. This type of program...

12. Antibodies 101: Conjugation

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    ...protein-protein interactions with other peptides. Created with BioRender.com.     Many biologists can...

13. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ...inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing TRIM21, finding...

14. Visualizing Protein Turnover In Situ

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    ...neurodegeneration. The level of this protein is decreased in Alzheimer’s disease patient’s brains. However...

15. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report electrical...

16. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    ...highlights of 2018 for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline...

17. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    ...proteins are available, and their number is steadily increasing. Of course, you want the ‘latest and greatest...

18. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...more efficient, as one does not have to thoroughly screen colonies for the insert. Gateway® technology (developed...

19. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ...limitations. New efforts have recently been made to increase cutting efficiency and diversify PAM sequences...

20. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...Roth BL (2007) Evolving the lock to fit the key to create a family of G protein-coupled receptors potently...