We narrowed to 962 results for: Cre

1. 5 Great Apps for Lab Life

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   Blog Post

   ...only need to skim the alphabet on the side of the screen to find the sequence for your enzyme of interest...

2. How to Lead a Great Meeting

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   Blog Post

   ...points. At Addgene, we like to do this live on the screen using a Google Doc. Action items: Before leaving...

3. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   Blog Post

   ...has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer comparing a standard...

4. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   ...many major cancer genes. These should be used to create ever more complete “look-up-tables” of mutant function...

5. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ...signaling pathway.  As a proof-of-principle, the team created an OptoNB to control the Ras/Erk signaling pathway...

6. AAV Q&A with Tim Miles

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   Blog Post

   ...flexibility of AAV genome design in remarkably creative ways. The continued development of more and smaller...

7. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   Blog Post

   ...leadership approach to each person, I was able to create a more productive and cohesive team environment...

8. Antibodies 101: Flow Cytometry Controls

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   ...cells but with far more predictability. The beads create an artificial positive (bound) and negative (unbound...

9. GCE4All: Making Genetic Code Expansion Accessible

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   ...The catch The rapid expansion of GCE led to the creation of many new tools and potential applications. ...

10. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post

    ...basic guidelines below, you’ll likely be able to create a small, usable panel.  First, fluorophores vary...

11. Antibodies 101: Isotypes

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    ... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

12. Plasmids 101: Methylation and Restriction Enzymes

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    Blog Post

    ...keep in mind that dam-/dcm- strains may have an increased rate of mutation (as these would also be deficient...

13. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    Blog Post

    ...harnesses the power of bacterial recombineering to create insertions, deletions, and substitutions - at the...

14. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ...Genome-Wide Control for Large-Scale Functional Screens        ...

15. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    Blog Post

    ...can make a big difference, if you can find one. Create your own peer mentoring circle. This type of program...

16. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    Blog Post

    ...inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing TRIM21, finding...

17. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

    ...proteins are available, and their number is steadily increasing. Of course, you want the ‘latest and greatest...

18. Visualizing Protein Turnover In Situ

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    ...neurodegeneration. The level of this protein is decreased in Alzheimer’s disease patient’s brains. However...

19. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    Blog Post

    ...toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report electrical...

20. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    Blog Post

    ...highlights of 2018 for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline...