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We narrowed to 1,031 results for: ELL

Showing: 881 - 900 of 1031 results
  1. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ... brain regions. You can partially control which cells are successfully infected by an AAV by altering ...control where your gene of interest is expressed.  A cell-type specific Cre can provide you with that control...
  2. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...mixture of two base editor enzymes in mammalian cells. Read the paper in bioRxiv Find Target-ACE   CRISPR...bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target activity ...
  3. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...pathways that are frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis...pathway. “The idea is that if you introduce them into cells one at a time, you could identify those that confer...
  4. Tag Your Favorite Yeast Genes with Ease

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    Blog Post
    ...insertion. The linear PCR product is introduced into the cell by direct transformation. A given insert will typically...protein-protein interactions via FRET. Sidae Lee, Wendell Lim, and Kurt Thorn at UCSF have recently developed...
  5. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...small Cas protein, is active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered ...demonstrating that CasX is active in both E. coli and human cells. Using the structure of CasX at eight different ...
  6. Viral Vectors 101: Parts of the AAV Packaging Plasmid

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    Blog Post
    ..., and VP3, which together form the outer capsid shell that protects the viral genome. The cap gene is ...for serotype-specific differences in tissue and/or cell type tropism. Introducing changes and/or short peptides...
  7. Antibodies 101: Chimeric Antibodies

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    Blog Post
    ... region of a different species and/or isotype (Liddell 2013). In most cases, the resulting chimeric antibody...antibodies! References and resources References Liddell, E. (2013). Antibodies. In The Immunoassay Handbook...
  8. Extracurricular Activities for a Strong Science Career Path

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    Blog Post
    ...industry?” Once academia was the clear answer with a well-defined path to professorship. But recently a downward... professions increases your chances of finding a well-suited job. EAs shouldn't be viewed as an unproductive...
  9. Pushing the Limits of DNA Assembly

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    Blog Post
    ...skip purification steps and end-repair steps with well-designed fragment overlap sections, while still ...of NEBuilder HiFi DNA assembly method As with any well-executed cloning endeavor, NEBuilder requires upfront...
  10. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    Blog Post
    ...measure the pH of our samples, we used a solution of cell culture medium, which is buffered and contains the... 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun MI. Protein...
  11. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...again or set up a meeting. If you really got along well, suggest a coffee chat in the near future. Even ...you succeed in any way. Don’t believe people who tell you that thank you isn’t necessary. I once got a...
Showing: 881 - 900 of 1031 results