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We narrowed to 1,147 results for: Gene

Showing: 881 - 900 of 1147 results

  1. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...https://www.addgene.org/browse/article/28248224/","href_with_scheme":"https://www.addgene.org/browse/article...University of Calgary. Over the past few decades, genetically encoded calcium indicators (GECIs) have become...Several OCaMP constructs are now available through Addgene. Whether you're working with cell cultures, slice..." %}{% raw %}"Find OCaMP plasmids available at Addgene!"{% endraw %}{% end_module_attribute %}{% module_attribute.../article/28248224/","type":"EXTERNAL"},"user_generated_content":false}{% endraw %}{% end_module_attribute...2025.07.28.667269. Additional Resources on the Addgene Blog Viral Vectors 101: Calcium Sensors New Neuroscience...Fluorescent Protein Additional Resources on addgene.org Fluorescent Proteins: Biosensor Plasmids In-...

  2. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...and Jennifer Doudna Labs used targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This nuclease...frequently used Cas9, spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s found in other...exhibit enhanced site accessibility In order to generate a genomic alteration at a specific location, it... sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9...the Jungjoon Lee Lab used directed evolution to generate Sniper-Cas9 resulting in a Cas9 with improved ...PMCID: PMC6255809.  Additional resources on the Addgene blog Read our CRISPR 101 blog posts Find more ... Download the CRISPR 101 eBook Resources on Addgene.org Visit our CRISPR plasmids and resources page...

  3. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...are tumor microenvironments made of?  A tumor’s genesis is divided into 3 phases: initiation, progression...that can be transferred to other cells, inducing epigenetic changes in targeted cells. In this case, exosomes...formation by inhibiting cell growth, invasion, and angiogenesis (Allinen et al., 2004). In fact, Sanford H. ...influence tumor microenvironments: immunology and angiogenesis (Witz et al., 2009). Then, scientists began ...Fernando Schmitt. "Microenvironment in breast tumorigenesis: Friend or foe?." Histology and histopathology...PubMed PMID: 20036813. Additional resources on the Addgene blog Read our cancer related blog posts Learn ...about the tumor microenvironment Resources on Addgene.org Visit our cancer research plasmids and resources...

  4. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...developed a dual virus system using both optogenetics and chemogenetics to study the activity of parvalbumin...in the lab. He has recently developed a third-generation intensiometric glutamate sensing fluorescent ...catalyzes the desulfuration of 3-mercaptopyruvate and generates an enzyme-bound hydropersulfide that is then transferred...withdrawal before they occur.  First, she used a genetic method to tag neurons activated by opioid withdrawal...light activates the neurons (via oChIEF) and a chemogenetic agonist inhibits the neuron (via hM4D). She ...postdoc at Harvard University where she engineers genetically encoded voltage indicators. Tian developed a ...optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage indicator which had improved ...

  5. We’re Going Viral This Week!

    Type
    Blog Post
    ...and learn more about Addgene’s viral service. Additional resources on the Addgene blog Download our Viral...service, and now we’re “going viral” again! #AddgeneGoesViral on Twitter Starting today, our Twitter feed... in the graphic below. Follow the hashtag #AddgeneGoesViral to find information on the basics of viral...applications, and some new resources available from Addgene. There’s a lot to talk about! And by the way, there...reagents and data, we’re also publishing research. Addgene’s research team also investigates new strategies... posts Learn more about AAVs Resources on the Addgene website Visit the AAV Data Hub Learn more about...

  6. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...mouse inner ear. Fourth-generation cytosine base editors The fourth-generation base editors, BE4, reduce...activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine base editing scope and efficiency...18 bases upstream of the PAM.  David Liu’s lab generated BE3 variants with other deaminases: AID, CDA1,...these three improvements represent the fourth generation of base editors. Compared to BE3, BE4 offers ... phage-assisted evolution selection systems to generate ABE8e(TadA-8e V106W), which edits ~590-fold faster...., 2020). This is important because ABEs have generally been slow, meaning that the Cas9 domain often ... N. M. (2023). Improved cytosine base editors generated from TadA variants. Nature Biotechnology, 41(5...

  7. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...Novartis, Biogen, Amgen, New England Biolabs, Regeneron, and Genentech, among others, and the list is growing...towards researching neurodegenerative diseases.   Additional resources on the Addgene blog Check out all...choosing a lab Resources on Addgene.org Find open positions at Addgene Check out our career videos ...beginning of March, and the jobs are mixed into the general careers section of their web site. Each position... a position open up. Other companies, such as Genentech, will not. The point is that each company has ...

  8. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...488nm blue light. The T203H mutation in PA-GFP generates a chromophore population that is more neutral,...PA-FPs by performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry...negative” photoswitching. In 2008, scientists used mutagenesis screens to find a version of Dronpa that doesn't...endogenous protein structure and function. By generating monomeric PA-FPs, there is less chance that the...induce though their continuous excitation. By generating PA-FPs fused to intracellular proteins, scientists...PubMed PMID:18724362. 8.Stiel, Andre C., et al. "Generation of monomeric reversibly switchable red fluorescent...PMC4344411. 13.Berlin, Shai, et al. "Photoactivatable genetically encoded calcium indicators for targeted neuronal...

  9. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ... use.  View the Addgene Plasmid Request Data Dashboard! Figure 1: The Addgene Requests Dashboard...I was excited to see that Addgene has recently added a new feature for depositing labs to its website...default. Depositors can download their CSV file from Addgene by following these instructions, and then uploading... 2: Insert showing the upload function for the Addgene Requests Dashboard After the upload, the name...displays the data in a standardized way. Since Addgene encourages the public sharing of aggregated data...Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes. You can follow him...

  10. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...Antibodies Resources on Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant...introduction, we broke down the method into six general steps and outlined the considerations to be made...fixation*. Organic Solvents Organic solvents, generally acetone, methanol, or ethanol, work by dehydrating...to use your specific antibody for IF, look for general IF protocols for your sample type. Starting with...cross-linking fixation; they require an additional step generally referred to as Antigen Retrieval. I chose not ...the-process-of-fixation-and-the-nature-of-fixatives/  Additional resources on the Addgene blog: Antibodies 101: Introduction to Immunofluorescence...recombinant antibodies at Addgene  ...

  11. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...Resources on Addgene.org Find protocols of molecular biology techniques Find plasmids at Addgene Make your...have integrations with a broad range of relevant generic and science-specific tools. In addition, it should...repositories Over the past decade or so a number of general purpose repositories have been launched and are... associated with publications into one of five general purpose open source data repositories: Dataverse...one and preferably more than one of these five general purpose repositories. RSpace has been integrated...@rory_macneil.     Additional resources on the Addgene blog Find tips on choosing an electronic lab notebook...your work more accessible by depositing at Addgene ...

  12. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...Resources at Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All ...and used in assays for over half a century; the Addgene blog has an excellent primer and tips from experts...can be created in many different ways, but the general premise is that an aptamer in its unbound state...likely to trigger immunoresponses. Nucleotides, in general, are far less immunogenic than proteins. Assays...ions or particularly small molecules that do not generate an immune response. Aptamers can be selected for..., Pages 1348-1353. Additional Resources on the Addgene Blog Tips for Using FRET in Your Experiments Learn...

  13. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... The HuidaGene Therapeutics team has deposited their editors, called gTBE and gCBE, with Addgene. gTBE...editors were developed, though, researchers at HuidaGene Therapeutics guessed that fusing MPG to an ABE...this promising start, the team used rational mutagenesis to produce the adenine transversion editor AYBEv3...and ABEs, they could be useful for saturation mutagenesis, and they provide a strong proof of concept for...empty space. Over several rounds of rational mutagenesis, the team progressed to gGBEv6.3, which was capable...s41467-024-50012-w Additional Resources on the Addgene Blog CRISPR 101: Cytosine and Adenine Base Editors...about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids for base editing Get ...

  14. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...publication. Who is to say that the initial data-generating lab has efficiently trawled their own data? A...much time they had. It could be argued that data generators may, in light of the policy, hold onto data longer... available to everyone at once, including the generators. This practice doesn’t appear to harm the originating...amounts of storage. For example, mouse behavioral geneticists may track the motion of multiple animals on different... different occasions via video, generating huge amounts of data. When published, this data is usually ...as SlideShare, Figshare and Dataverse. Just as Addgene helps with DNA reagents, these resources can offload...just a single projection of it, could allow idea generation and extension, based on existing, published data...

  15. Science Communication for Everyone

    Type
    Blog Post
    ...science communication Resources on Addgene.org Learn how Addgene engages with the community ... Addictive Brain and science communication for general audiences. How does The Addictive Brain address...findings, and experiments as articles written for general audiences. So far, we are beginning to make an ...science, especially disease related topics. Neurodegenerative and brain disorders are frequently overshadowed...“manipulate” could mean “illicit tampering” to general audiences while “manipulate” could mean “worked...BioTechniques.     Additional resources on the Addgene blog Find all of our science communication posts...

  16. Editor's Choice, September 2016

    Type
    Blog Post
    ... that we once again reached new heights on the Addgene blog: we surpassed 60,000 views for the month of... would love to see more posts like this on the Addgene blog. Read the post for the full details and many...,” was contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher...of therapeutics. It also represents a first for Addgene - while we often participate in 5Ks throughout ...Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific...Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the Research Community...Francisco and would like to give my thanks to all the Addgene supporters who spoke with me and deposited plasmids...

  17. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...communicating science to the general public and training the next generation of science communicators. In...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... is a source of scientific information for the general public as well as a platform for scientists to ...discuss the importance of actionability on the Addgene blog and how SITN short-form blog posts help readers...Additional Resources on the Addgene Blog Resume Writing for Non-academic Science Careers 10 Steps to a Perfect...

  18. March for Science

    Type
    Blog Post
    ...author do not represent a formal stance taken by Addgene or its staff. In Washington, D.C. as well as sister... in education, government, and civilization in general (1). Science and politics? Science is an apolitical...as evidence of climate change, the safety of genetically modified organisms (GMOs), the absence of a link... human consumption in comparison to 37% of the general public (5, 6). Similarly 90% of active research...mostly due to human activity while much less of the general public agrees (5, 6). Many of the new administration...them. Other popular reasons to march include: General inclusion of science to inform policy (17). The...relatable (17, 20). This is an effort to show the general public that science is done by people like them...

  19. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...associated with the brain’s reward circuits are generally linked to dopamine (DA), a well-known reward chemical...in freely moving mice Record DA dynamics of optogenetically activated and inhibited neurons in live mice...neuronal imaging of dopamine dynamics with designed genetically encoded sensors." Science 360.6396 (2018): eaat4422... PMCID: PMC6287765. Sun, Fangmiao, et al. "A genetically encoded fluorescent sensor enables rapid and ...PMCID: PMC6092020. Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

  20. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research...familiar with the alarming amount of waste that is generated. This is a systemic problem that we need to address...to learn about practices I could bring both to Addgene, and share with my peers. In fact, there was so...account for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled, ... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...
Showing: 881 - 900 of 1147 results