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We narrowed to 1,149 results for: Gene

Showing: 881 - 900 of 1149 results

  1. Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Check out additional microbiology resources Stem cell models for...additional plant biology blog posts Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

  2. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...particularly genetics research, for decades. Its short life cycle, reasonably close genetic homology to...demonstrate the new system in a video filmed at Addgene:     If you're working in fly research, I hope...References and Resources Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References...

  3. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...research and to keep useful discoveries coming. At Addgene, we’re huge supporters of science communication...Additional Resources on the Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about...

  4. Luciferase Plasmid Collection

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    Collection
    ...Explore Addgene's collection of luciferase plasmids for gene expression assays and bioluminescent reporters...luciferase-containing plasmid and the effects on gene expression can be measured by the luminescence output...into which you can clone your regulatory element or gene of interest into to create a luciferase reporter...Firefly, Renilla Creating ORF-Firefly luciferase gene fusions. Renilla luciferase under the control of...enhancer regions. Mutations within the luciferase gene allow for increased copy number in E. coli and expression... Retroviral expression of firefly luciferase and gene of interest Scott Lowe 21375 pHIV-Luciferase Firefly...Firefly Lentiviral vector for insertion of your gene of interest upstream of IRES-firefly luciferase. Bryan...

  5. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...We’re big fans of all our depositors here at Addgene — your contributions make the repository what it... designing a novel topology, then iteratively generated 3D models and amino acid sequences that got closer...Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools like high resolution Ras...crosslinked peptides proposed as a scaffold for a new generation of drugs, all developed with their protein design...

  6. CRISPR Plasmids - Tagging

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    Collection
    ...listed below. When targeting other gene loci, you will prepare the gene-specific CRISPR and donor vectors...multiple genes. Plasmids can be found associated with the following article: Paix, et al. Genetics 2014 Allen...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...repair template design. How to use CRISPR to tag your gene of interest Mendenhall and Myers Tagging System ... the plasmids as listed in each row. If your own gene of interest is currently unavailable, you will need...terminal affinity tag (3xFLAG-2xSTREP) on endogenous genes for the isolation of native protein complexes. This... is different from the conventional HDR-mediated gene knock-in. The detailed procedure can be found in...

  7. 2018 #DeckTheLab Contest Winners

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    Blog Post
    ...signs of holiday spirit still linger on around the Addgene office as we peruse the #DeckTheLab submissions...MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan... @EmoryChem lab office looking festive 🎄🌟🎁 @Addgene #DeckTheLab pic.twitter.com/bDRqAKrWHz — Dyer Lab...winners will receive a holiday care package from Addgene. We hope all of our entrants had fun decorating...

  8. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    ...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Learn...approaches for lab sustainability Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

  9. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ... event – all while keeping the party going! At Addgene, we’ve set-up an exhibitor booth in order to help...all the words of support from scientists using Addgene in their labs. And it has been exciting to learn... I heard an impressive talk about the use of optogenetics tools to inhibit epileptic seizures in a mouse...McGovern Institute for Brain Research) – and an Addgene depositing scientist – stop by our booth to say...

  10. Optogenetics Plasmid Collection

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    Collection
    ... AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...transcription", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Return to top Content...Collections Optogenetics Plasmids Optogenetics Plasmid Collection You may also like... Optogenetics Guide AAV...tables, refer to Addgene's Optogenetics Guide . Interested in more news about optogenetics research? Find... AAV Viral Service Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial...Find optogenetics plasmids such as microbial opsins and optical switch tools to activate, inhibit, and...Microbial Opsin Plasmids Optical Switch Plasmids Optogenetics plasmids encode proteins that are controlled...

  11. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...protein (FP) tags. Information on genes and associated neurodegenerative diseases was curated from several...but not exhaustive. If you do not see a particular gene of interest, please search our full plasmid collection... Huntington's Disease Parkinson's Disease Others Gene Type Wildtype Mutant Partial Sequence Codon-Optimized... T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease PI 1177 p416 25Q GPD HTT GFP... Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a ...Spinal muscular atrophy Eugene Yeo 156073 TARDBP TARDBP V5-MCP EF1a ALS Eugene Yeo 156883 pD649-HAsp-CSF1R-Fc...Parkinson's, FTD Eugene Yeo 214673 lucMAPT-30U MAPT T5, luciferase CMV Parkinson's, FTD Eugene Yeo 214674 lucMAPT-GenRep...

  12. CRISPR Plasmids - Epigenetics

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    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert...methylation Epigenetic modification is typically targeted to a specific promoter or enhancer for your gene of ...CRISPR Epigenetics CRISPR Plasmids: Epigenetics Mammalian Plant Resources To make targeted epigenetic modifications... plasmids designed for epigenetic modification. To learn more about epigenetic modification with CRISPR...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...have fused catalytically dead Cas9 (dCas9) to epigenetic modifiers. Common modifiers include: P300 — activation... local chromatin state. Figure 1: Overview of epigenetic modification using CRISPR. Created with BioRender.com...

  13. Chemogenetics AAV Preps

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    Collection
    ...Service AAV Chemogenetics Viral Vector Packaging Service: Chemogenetics AAV Use our chemogenetics AAV to chemically...Ready-to-use AAV available from Addgene's viral service encoding chemogenetics tools. Viral...in specific cell types. See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information... Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t See What You’re Looking For? Our Packaged...

  14. Optogenetics AAV Preps

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    Collection
    ...Packaging Service AAV Optogenetics Viral Vector Packaging Service: Optogenetics AAV Optogenetic tools can be used... potentials in neurons. See our Optogenetics Plasmids and Optogenetics Guide page for more information...ready-to-use AAV preparations of optogenetics plasmids available in Addgene's collection. Opsin Excitatory...select plasmids in the repository. Browse our optogenetics AAV collection, including channelrhodopsins,...control cell activity in living tissue with light. Optogenetics is typically used in neuroscience to control...consider using fewer filtering options. Browse all Optogenetics AAV *AAVrg = retrograde serotype, produced with...

  15. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

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    Blog Post
    ...KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded fluorescent kinase reporters. A KTR is...custom KTR design, plasmids are available from Addgene! References Kudo, T., Jeknić, S., Macklin, D.N...KTR plasmids here! Additional Resources on the Addgene Blog Learn about photoswitchable kinases Learn...protein phosphorylation Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors...

  16. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...fluorescent microscopy technique for your study see Addgene’s blog post Which Fluorescence Microscopy Techniques...experiment and are unsure which to choose, check out Addgene’s article: Which Fluorescent Protein Should I Use.... During FACS, the sample stream oscillates to generate droplets that are charged as they pass through.../s10616-011-9415-0 Additional resources on the Addgene blog Check out other methods using fluorescent...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...

  17. Antibodies 101: Designing Your First Flow Panel

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    Blog Post
    ...Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...fluorescent reporter, such as GFP or mCherry, through genetic modification. As your protein of interest will ...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Introduction to Gating in ...

  18. Reflections and Looking Ahead

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    Blog Post
    ...power of community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific collaboration...sequencing capabilities to complement our next-generation sequencing, setting new standards for plasmid...online and in-person, you helped us understand how Addgene can better serve the global scientific community...about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez...

  19. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Find...Find lab tips for your research Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

  20. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...protein. The pCri system, recently deposited to Addgene by F. Xavier Gomis-Rüth, aims to take the guesswork... either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The ...visualization of the data from RSCB PDB Resources on the Addgene Blog Plasmids 101: E. coli strains for protein...expression Plasmids 101: Protein tags Resources on Addgene.org Find Additional Cloning Kits ...
Showing: 881 - 900 of 1149 results