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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 982 results for: IGH@

Showing: 881 - 900 of 982 results

  6. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...interneurons. Take a look to see if these new preps might enhance your research!   Table 1: New ready-to-use...

  7. Editor's Choice, October 2016

    Type
    Blog Post
    ...for detecting genome edits, to discover why you might want to use Reddit to discuss your science and more...

  9. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...controller Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5mL...sample of possible dilutions. You may want to try higher/lower dilutions depending on your downstream applications...transduced with lower dilutions of the virus will have higher levels of expression. Consider expanding populations...population may drop. This is because cells that express high levels of the transgene may have reduced growth ...

  12. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...counter Reagents Polyclonal stable cell pool DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5...for one 96-well plate. Allow the cells to grow overnight. Replace the medium on the cells and collect the...conditioned medium, it will be more accurate if a higher volume of homogenized cell solution is transferred... Western blotting to screen for lines with the highest or lowest transgene expression ( Figure 2 ). Sample...

  15. AAV Production in HEK293 Cells

    Type
    Protocol
    ...PES. Using a PES filter will maximize titer . DMEM high glucose, Corning 10-013-CV DMEM, low glucose (1 ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5...dissolved. Pro-Tip This step is challenging due to the high viscosity of PEG. Stirring under medium heat will...precipitation. Precipitation of the viruses can proceed overnight at 4 °C if needed. Transfer the entire sample ... prep . The clarified supernatant can be kept overnight at 4 °C before proceeding with the purification...

  17. Reflections and Looking Ahead

    Type
    Blog Post
    ...contributions to key research papers are already inspiring higher standards in antibody use (Ayoubi et al., 2024;...

  18. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...Inoculating an Overnight Liquid Culture . After you have bacterial growth, add 500 μL of the overnight culture...bacteria onto an LB agar plate . Grow your bacteria overnight at the appropriate temperature. Growth conditions...page. The next day you will be able to start an overnight culture for plasmid DNA prep the following day...

  20. Science as Art

    Type
    Blog Post
    ...microscope, you too can become an artist. Scientists moonlighting as artists can compete in the Nikon Small World...
Showing: 881 - 900 of 982 results