We narrowed to 1,018 results for: LEA
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TypeBlog Post...sequence with one or more enzymes. Click here to learn how to run an agarose gel We’ve worked with...
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Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...29300010. Additional Resources on the Addgene Blog Learn more about in vivo imaging tools The luciferase ... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post... to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral service Find optogenetics... -
10 Ways to Share Your Science!
TypeBlog Post...communicating your research by guest blogging here, just leave a comment if you're interested and our team will... -
Cloning
TypeGuide...molecular biology enzymes: 5' exonuclease, polymerase and ligase. 5' exonuclease digests the 5' end of dsDNA...properties of type IIS restriction endonucleases. These endonucleases cut dsDNA at a specified distance...) relies on the 3'-5' exonuclease activity of T4 DNA polymerase. An exonuclease is an enzyme which removes...Restriction Enzyme Cloning Restriction enzyme (endonuclease) based molecular cloning is the "classic" cloning...naturally produced by certain bacteria and archaea, cleave double stranded DNA (dsDNA) at specific sequence...cloning project. Restriction enzyme cloning also leaves behind a short scar in the DNA sequence and can...cloning utilizes the Taq polymerase which naturally leaves a single adenosine (A) overhang on the 3' end of... -
Viral Vectors 101: Transductions
TypeBlog Post...genetic cargo — the viral genome — is swapped, at least partially, for a nucleic acid cargo of your choice... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...reliable optically evoked neuron activity for at least several months after implantation of fibers, indicating... -
Antibodies 101: Epitope Tags
TypeBlog Post...suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out Addgene’s monoclonal... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post...antibodies may be the right solution for you. Read on to learn more. What is an isotype? Antibodies are composed... -
Summer SciComm: Science Storytelling
TypeBlog Post... science communication and can use what you've learned to share your science with others. My favorite... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post... Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog Post...I knew anything about being an editor - that I learned on the job. The day-to-day work of a science editor... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...PMC6610143. Additional resources on the Addgene blog Learn about visualizing protein turnover Download the ... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog Post... research materials as quickly as possible. (To learn more about why MTAs matter, check out another blog... -
Plasmids 101: Origin of Replication
TypeBlog Post... any questions? Need something clarified? If so please, post in the comments. References Chatterjee A... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...for scientists that helps them share reagents — leading to open, reproducible science,” said CZI Head of... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable single ... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog Post... the preprint repository BioRxiv. This example clearly illustrates the power of collaboration, which despite... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...Read more about COVID-19 databases and resources Learn about Addgene's work to share plasmids to all scientists... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog Post...the best way to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps ...