We narrowed to 1,019 results for: REN;
-
TypeBlog Post...same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques? Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination...
-
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...experiment. Good luck with your experiment! References Bikard D, Jiang W, Samai P, Hochschild A, Zhang...dCas9-CBP and the SAM system differ in target preference. Sci Rep 9: . https://doi.org/10.1038/s41598-... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...more precise editing in the lab. References and resources References Dong D, Guo M, Wang S, Zhu Y, Wang... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...2020). The generated ABE8s needed to produce 3 concurrent base edits to withstand antibiotic selection....great addition to the base editing toolbox. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...-Wagner’s lab produce different levels of dCas9 expression and contain different antibiotic resistance... -
Viral Vectors 101: Virus Safety
TypeBlog Post...dividing up the essential components of a virus into different plasmids. For a retroviral system this looks like... health and safety department if they are not currently provided. Eye protection or face shields are ... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post... discrimination is especially important in the current outbreak because of the neurological symptoms associated...applications in health, energy, and sustainability. References 1. Pardee, Keith, et al. "Rapid, Low-Cost Detection... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...will produce the same unique SapI 5’ overhang, different combinations of connector and tag donor plasmids...light on previously unknown protein functions. References Schwartz, Matthew L. and Eric M. Jorgensen. “... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...immunoprecipitate the entire complex in three different cell lines. The Gräslund lab plans to continue...of indels to measure time. These plasmids with different promoters driving expression of Cas9 were used... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...science. It's open-source. You can see all the current code on GitHub, ask us questions about it, or send.... It builds on existing popular web services, currently Crispor and Crispresso, that adhere to the same... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post... new eBook! Our goal was to create a one-stop reference guide for plasmids. We've combined our Plasmids...solutions necessary for advancing the field. References: 1. Internal initiation of translation of eukaryotic... -
Which Fluorescent Protein Should I Use?
TypeBlog Post... acidic, or synaptic vesicles). Some FPs have different ex/em spectra (e.g. mKeima) or change fluorescent...Fluorescent Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et. al. (2008) “Fluorescent... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...functions, which we know and love. Boolean is in reference to George Boole who in 1854 was thinking about...tell us readers, what do you want to make? References: Jerome Bonnet et al. "Amplifying genetic logic... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...about science whenever he's not off the grid. References Bischof J, Duffraisse M, Furger E, Ajuria L, ...plasmids and resources Visit our Molecular Biology Reference ... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...enjoy and that plays at least slightly to your strengths. Otherwise, you’re going to drop your project ...scientific training. Cartoons may be read as “for children” or may need text for audience interpretation.... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...decorative" title="Check icon" %} References and Resources References Hwang, J. M., Piccinini, T. E., Lammel... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...human genome-wide CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do...conditions such as model systems and primary cells. References 1. Doench, John G., et al. "Rational design of... -
Hot Plasmids Spring 2024
TypeBlog Post...editing efficiencies in a number of cell types at different genomic loci — in some cases, over 20-fold higher...prime editor. B, Prime editing efficiencies at different loci in U2OS cells. Image adapted from Yan et ... -
Viral Production at Addgene
TypeBlog Post... by Ina Ersing in August 2023. Resources and references Resources at the Addgene blog Find and share ...Quantification Viral Vectors 101: An Introduction to AAV References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson... -
How to Be an Excellent Trainee
TypeBlog Post...protocol you don’t understand the purpose of, or a different way of doing something then you’ve done previously...an active partner Everyone’s learning style is different, so let your mentor know what yours is! For instance...