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Showing: 881 - 900 of 1019 results
  1. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination...
  2. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...dividing up the essential components of a virus into different plasmids. For a retroviral system this looks like... health and safety department if they are not currently provided. Eye protection or face shields are ...
  3. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ... discrimination is especially important in the current outbreak because of the neurological symptoms associated...applications in health, energy, and sustainability.   References 1. Pardee, Keith, et al. "Rapid, Low-Cost Detection...
  4. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post
    ...will produce the same unique SapI 5’ overhang, different combinations of connector and tag donor plasmids...light on previously unknown protein functions. References Schwartz, Matthew L. and Eric M. Jorgensen. “...
  5. Hot Plasmids and Viral Preps - May 2021

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    Blog Post
    ...immunoprecipitate the entire complex in three different cell lines. The Gräslund lab plans to continue...of indels to measure time. These plasmids with different promoters driving expression of Cas9 were used...
  6. Plasmids 101: Multicistronic Vectors

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    Blog Post
    ... new eBook! Our goal was to create a one-stop reference guide for plasmids. We've combined our Plasmids...solutions necessary for advancing the field. References: 1. Internal initiation of translation of eukaryotic...
  7. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... acidic, or synaptic vesicles). Some FPs have different ex/em spectra (e.g. mKeima) or change fluorescent...Fluorescent Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et. al. (2008) “Fluorescent...
  8. Summer SciComm Series: Modes of Communication

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    Blog Post
    ...enjoy and that plays at least slightly to your strengths. Otherwise, you’re going to drop your project ...scientific training. Cartoons may be read as “for children” or may need text for audience interpretation....
  9. Hot Plasmids Spring 2024

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    Blog Post
    ...editing efficiencies in a number of cell types at different genomic loci — in some cases, over 20-fold higher...prime editor. B, Prime editing efficiencies at different loci in U2OS cells. Image adapted from Yan et ...
  10. Viral Production at Addgene

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    Blog Post
    ... by Ina Ersing in August 2023. Resources and references Resources at the Addgene blog Find and share ...Quantification Viral Vectors 101: An Introduction to AAV References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson...
  11. How to Be an Excellent Trainee

    Type
    Blog Post
    ...protocol you don’t understand the purpose of, or a different way of doing something then you’ve done previously...an active partner Everyone’s learning style is different, so let your mentor know what yours is! For instance...
Showing: 881 - 900 of 1019 results